Wnt10b (NM_011718) Mouse Tagged ORF Clone
CAT#: MR224739
- TrueORF®
Wnt10b (Myc-DDK-tagged) - Mouse wingless related MMTV integration site 10b (Wnt10b)
ORF Plasmid: tGFP
"NM_011718" in other vectors (4)
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CNY 3,656.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Wnt12 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224739 representing NM_011718
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGAGGAGCCCCGGTCTCGGCCTCCGCCCTTAGGCCTCGCGGGTCTCCTGTTCTTGGCTTTGTTCA GTCGGGCTCTAAGCAATGAGATTCTGGGCCTTAAACTTCCCGGTGAGCCGCCGCTGACGGCCAACACCGT GTGCTTGACCCTGTCCGGACTGAGTAAGCGACAGCTGGGGCTGTGCCTGCGCAGCCCCGACGTGACGGCG TCGGCGCTCCAGGGGCTGCACATCGCCGTTCACGAGTGTCAGCACCAGCTGCGCGACCAGCGCTGGAACT GCTCGGCACTGGAGGGCGGCGGCCGGCTGCCGCACCACAGCGCCATCCTCAAGCGCGGTTTCCGTGAGAG TGCTTTCTCCTTCTCCATGCTGGCTGCTGGGGTCATGCATGCTGTTGCCACAGCCTGCAGCCTGGGCAAG CTGGTGAGCTGCGGCTGCGGATGGAAGGGTAGTGGTGAGCAAGACCGGCTTAGAGCCAAGCTGCTGCAGC TTCAGGCACTGTCTCGGGGCAAGACTTTCCCCATCTCCCAGCCCAGCCCTGTTCCTGGCTCAGTCCCCAG CCCCGGCCCCCAGGACACGTGGGAATGGGGTGGCTGTAACCACGACATGGACTTCGGAGAGAAGTTCTCT CGGGATTTCTTGGATTCCAGGGAGGCTCCCCGGGACATCCAGGCGAGAATGCGGATCCACAACAACAGGG TGGGACGCCAGGTGGTAACGGAAAACCTGAAGCGGAAGTGCAAATGCCATGGAACGTCAGGCAGCTGCCA ATTCAAGACCTGTTGGAGGGCAGCGCCAGAGTTCCGGGCCATCGGGGCAGCACTGAGGGAGCGGCTGAGC AGAGCCATCTTTATCGATACCCACAACCGCAACTCTGGAGCGTTCCAGCCCCGCCTACGTCCGCGGCGCC TCTCTGGAGAGCTGGTTTACTTTGAGAAGTCTCCTGACTTCTGCGAGCGAGACCCTACTCTGGGCTCCCC AGGCACGAGAGGCCGGGCTTGCAACAAGACCAGCCGCCTCTTGGATGGCTGTGGCAGCCTGTGCTGTGGC CGTGGGCACAACGTGCTCCGGCAGACGCGAGTGGAGCGCTGCCACTGTCGTTTCCACTGGTGCTGTTATG TGCTGTGTGATGAGTGTAAAGTCACAGAGTGGGTCAATGTGTGTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224739 representing NM_011718
Red=Cloning site Green=Tags(s) MLEEPRSRPPPLGLAGLLFLALFSRALSNEILGLKLPGEPPLTANTVCLTLSGLSKRQLGLCLRSPDVTA SALQGLHIAVHECQHQLRDQRWNCSALEGGGRLPHHSAILKRGFRESAFSFSMLAAGVMHAVATACSLGK LVSCGCGWKGSGEQDRLRAKLLQLQALSRGKTFPISQPSPVPGSVPSPGPQDTWEWGGCNHDMDFGEKFS RDFLDSREAPRDIQARMRIHNNRVGRQVVTENLKRKCKCHGTSGSCQFKTCWRAAPEFRAIGAALRERLS RAIFIDTHNRNSGAFQPRLRPRRLSGELVYFEKSPDFCERDPTLGSPGTRGRACNKTSRLLDGCGSLCCG RGHNVLRQTRVERCHCRFHWCCYVLCDECKVTEWVNVCK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011718 |
ORF Size | 1167 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011718.2, NP_035848.1 |
RefSeq Size | 2272 bp |
RefSeq ORF | 1170 bp |
Locus ID | 22410 |
UniProt ID | P48614 |
MW | 43.6 kDa |
Gene Summary | Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Progenitors oppositely polarize WNT activators and inhibitors to orchestrate tissue development
,null,
eLife
,PubMed ID 32310087
[Wnt10b]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209647 | Wnt10b (untagged) - Mouse wingless related MMTV integration site 10b (Wnt10b), (10ug) |
CNY 3,656.00 |
|
MG224739 | Wnt10b (tGFP-tagged) - Mouse wingless related MMTV integration site 10b (Wnt10b), (10ug) |
CNY 3,710.00 |
|
MR224739L3 | Lenti ORF clone of Wnt10b (Myc-DDK-tagged) - Mouse wingless related MMTV integration site 10b (Wnt10b) |
CNY 5,230.00 |
|
MR224739L4 | Lenti ORF clone of Wnt10b (mGFP-tagged) - Mouse wingless related MMTV integration site 10b (Wnt10b) |
CNY 6,056.00 |