TMLHE (NM_018196) Human Tagged ORF Clone
CAT#: RC214667
- TrueORF®
TMLHE (Myc-DDK-tagged)-Human trimethyllysine hydroxylase, epsilon (TMLHE), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid: tGFP
"NM_018196" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AUTSX6; BBOX2; TMLD; TMLH; TMLHED; XAP130 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214667 representing NM_018196
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTACCACAGATTGTCCCACCTACACAGCAGGCTTCAGGACTTGCTGAAGGGAGGAGTCATATATC CGGCCCTTCCACAGCCCAACTTCAAAAGCTTACTTCCTTTAGCTGTCCATTGGCACCATACAGCCTCCAA GTCTCTGACTTGTGCTTGGCAGCAACATGAAGATCATTTTGAGCTGAAATATGCTAATACCGTGATGCGC TTTGATTACGTCTGGCTTCGAGACCACTGCCGCTCAGCATCGTGCTACAACTCTAAGACTCACCAGCGCA GCCTGGATACTGCCAGTGTGGATTTATGTATCAAGCCAAAGACCATTCGTCTGGATGAGACCACACTCTT TTTCACTTGGCCAGATGGTCATGTGACTAAATATGATTTGAATTGGCTGGTGAAAAACAGCTATGAAGGG CAGAAACAAAAAGTCATCCAGCCTAGAATACTATGGAATGCTGAAATCTACCAGCAAGCCCAAGTTCCAT CGGTAGATTGCCAGAGCTTCTTAGAAACCAACGAGGGACTGAAGAAGTTTCTGCAAAACTTTCTGCTCTA TGGAATTGCATTCGTAGAAAATGTCCCTCCCACTCAAGAGCACACAGAGAAGTTGGCAGAAAGGATCAGC TTAATCAGAGAAACCATTTATGGGAGGATGTGGTATTTCACTTCAGACTTCTCCAGAGGTGACACTGCGT ACACCAAGCTAGCTCTGGATCGGCACACTGACACTACCTATTTTCAAGAGCCCTGTGGCATTCAAGTGTT TCATTGTCTTAAACATGAAGGAACTGGTGGCAGGACACTGCTAGTAGATGGATTCTATGCAGCAGAACAG GTACTTCAAAAGGCACCTGAGGAATTTGAACTCCTCAGTAAAGTGCCATTGAAGCATGAATATATTGAAG ATGTTGGAGAATGTCACAACCACATGATTGGGATTGGGCCAGTCTTAAATATCTACCCATGGAATAAAGA GCTGTATTTGATCAGGTACAACAACTATGACCGGGCTGTCATCAATACCGTTCCTTATGATGTCGTCCAT CGCTGGTATACAGCACACCGGACTCTAACGATAGAGTTGAGGAGACCTGAGAATGAGTTTTGGGTCAAAC TAAAGCCTGGCAGGGTCCTATTTATAGACAACTGGCGTGTCCTACATGGCAGGGAATGCTTCACTGGCTA CCGCCAACTGTGTGGCTGCTATTTAACAAGAGATGATGTATTAAACACTGCTCGCCTCTTGGGGCTTCAG GCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214667 representing NM_018196
Red=Cloning site Green=Tags(s) MWYHRLSHLHSRLQDLLKGGVIYPALPQPNFKSLLPLAVHWHHTASKSLTCAWQQHEDHFELKYANTVMR FDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTIRLDETTLFFTWPDGHVTKYDLNWLVKNSYEG QKQKVIQPRILWNAEIYQQAQVPSVDCQSFLETNEGLKKFLQNFLLYGIAFVENVPPTQEHTEKLAERIS LIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLKHEGTGGRTLLVDGFYAAEQ VLQKAPEEFELLSKVPLKHEYIEDVGECHNHMIGIGPVLNIYPWNKELYLIRYNNYDRAVINTVPYDVVH RWYTAHRTLTIELRRPENEFWVKLKPGRVLFIDNWRVLHGRECFTGYRQLCGCYLTRDDVLNTARLLGLQ A TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018196 |
ORF Size | 1263 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018196.4 |
RefSeq Size | 3961 bp |
RefSeq ORF | 1266 bp |
Locus ID | 55217 |
UniProt ID | Q9NVH6 |
Domains | Gamma-BBH |
Protein Pathways | Lysine degradation |
MW | 50 kDa |
Gene Summary | This gene encodes the protein trimethyllysine dioxygenase which is the first enzyme in the carnitine biosynthesis pathway. Carnitine play an essential role in the transport of activated fatty acids across the inner mitochondrial membrane. The encoded protein converts trimethyllysine into hydroxytrimethyllysine. A pseudogene of this gene is found on chromosome X. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214667L3 | Lenti-ORF clone of TMLHE (Myc-DDK-tagged)-Human trimethyllysine hydroxylase, epsilon (TMLHE), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
RC214667L4 | Lenti-ORF clone of TMLHE (mGFP-tagged)-Human trimethyllysine hydroxylase, epsilon (TMLHE), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
RG214667 | TMLHE (tGFP-tagged) - Human trimethyllysine hydroxylase, epsilon (TMLHE), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,256.00 |
|
SC111322 | TMLHE (untagged)-Human trimethyllysine hydroxylase, epsilon (TMLHE), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 3,656.00 |