INS-IGF2 (NM_001042376) Human Tagged ORF Clone
CAT#: RC220218
INS (Myc-DDK-tagged)-Human INS-IGF2 readthrough (INS-IGF2), transcript variant 2
ORF Plasmid: tGFP
"NM_001042376" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | INSIGF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220218 representing NM_001042376
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCCAGCCGCAG CCTTTGTGAACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGG CTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGCCTCAGCTTTGTCCCTCTCCTCC TCCACGTCAACCTGGCCAGAGGGTCTGGACGCCACAGCCAGGGCACCCCCTGCTTTGGTGGTGACTGCTA ATATTGGCCAGGCCGGCGGATCATCGTCCAGGCAGTTTCGGCAGAGAGCCTTGGGCACCAGTGACTCCCC GGTCCTCTTTATCCACTGTCCAGGAGCTGCGGGGACTGCGCAGGGACTAGAGTACAGGGGCCGAAGAGTC ACCACCGAGCTTGTGTGGGAGGAGGTGGATTCCAGCCCCCAGCCCCAGGGCTCTGAATCGCTGCCAGCTC AGCCCCCTGCCCAGCCTGCCCCACAGCCTGAGCCCCAGCAGGCCAGAGAGCCCAGTCCTGAGGTGAGCTG CTGTGGCCTGTGGCCCAGGCGACCCCAGCGCTCCCAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220218 representing NM_001042376
Red=Cloning site Green=Tags(s) MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQASALSLSS STSTWPEGLDATARAPPALVVTANIGQAGGSSSRQFRQRALGTSDSPVLFIHCPGAAGTAQGLEYRGRRV TTELVWEEVDSSPQPQGSESLPAQPPAQPAPQPEPQQAREPSPEVSCCGLWPRRPQRSQN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001042376 |
ORF Size | 600 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001042376.3 |
RefSeq Size | 828 bp |
RefSeq ORF | 603 bp |
Locus ID | 723961 |
UniProt ID | F8WCM5 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
MW | 21.4 kDa |
Gene Summary | This locus includes two alternatively spliced read-through transcript variants which align to the INS gene in the 5' region and to the IGF2 gene in the 3' region. One transcript is predicted to encode a protein which shares the N-terminus with the INS protein but has a distinct and longer C-terminus, whereas the other transcript is a candidate for nonsense-mediated decay (NMD). The transcripts are imprinted and are paternally expressed in the limb and eye. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220218L3 | Lenti ORF clone of Human INS-IGF2 readthrough (INS-IGF2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC220218L4 | Lenti ORF clone of Human INS-IGF2 readthrough (INS-IGF2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG220218 | INS (tGFP-tagged) - Human INS-IGF2 readthrough (INS-IGF2), transcript variant 2 |
CNY 4,370.00 |
|
SC311232 | INS (untagged)-Human INS-IGF2 readthrough (INS-IGF2), transcript variant 2 |
CNY 3,990.00 |
其它INS-IGF2产品