CD299 (CLEC4M) (NM_001144909) Human Tagged ORF Clone
CAT#: RC227458
- TrueORF®
CLEC4M (Myc-DDK-tagged)-Human C-type lectin domain family 4, member M (CLEC4M), transcript variant 9. Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_001144909" in other vectors (2)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CD209L; CD299; DC-SIGN2; DC-SIGNR; DCSIGNR; HP10347; L-SIGN; LSIGN |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC227458 ORF sequence, codon optimized.
Due to the complexity of NM_001144909, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGACTCTAAAGAGCCCAGAGTGCAACAGTTGGGCCTTCTTGAGGAGGACCCAACCACCAGCGGGA TTAGACTTTTCCCAAGGGATTTTCAGTTTCAGCAGATCCATGGACATAAGAGCTCAACGGGATGCCTTGG TCACGGCGCTCTTGTCCTCCAACTGCTGTCTTTTATGCTGCTGGCGGGCGTGCTCGTGGCCATTCTTGTT CAAGTTTCTAAAGTGCCTTCATCACTGTCTCAGGAGCAGTCAGAGCAGGACGCTATCTATCAAAACCTTA CCCAGTTGAAAGCCGCTGTTGGAGAACTGTCCGAAAAGTCCAAGTTGCAGGAGATCTATCAGGAGCTCAC ACAGCTGAAAGCTGCTGTTGGCGAGCTCCCCGAGAAAAGCAAGCTCCAAGAGATTTATCAGGAGCTGACA AGGCTGAAGGCTGCAGTGGGAGAGCTGCCAGAGAAAAGTAAGTTGCAGGAGATTTATCAAGAACTGACTA GGCTTAAAGCCGCTGTCGGCGAGCTGCCTGAGAAGAGCAAATTGCAGGAAATTTACCAAGAACTTACACA GCTTAAGGCTGCAGTCGGCGAACTCCCGGACCAGTCCAAACAGCAACAGATTTACCAGGAATTGACTGAC CTGAAAACCGCCTTCGAAAGGCTGTGCAGACATTGCCCAAAGGATTGGACTTTCTTCCAGGGAAATTGTT ATTTCATGAGTAATAGTCAGAGAAATTGGCACGATTCAGTGACGGCCTGTCAGGAAGTTAGGGCGCAGCT TGTGGTTATTAAAACCGCCGAGGAGCAGAACTTCCTGCAGCTGCAGACTAGTCGGTCCAACAGATTCAGT TGGATGGGCTTGAGCGATCTCAACCAGGAGGGCACTTGGCAGTGGGTGGACGGGAGCCCCTTGAGTCCGT CATTTCAGAGATATTGGAATTCTGGGGAGCCGAATAACAGCGGCAACGAGGATTGCGCCGAGTTCTCTGG CAGCGGGTGGAACGACAATCGCTGCGACGTGGACAACTATTGGATTTGTAAGAAGCCCGCCGCCTGTTTT AGAGATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227458 representing NM_001144909
Red=Cloning site Green=Tags(s) MSDSKEPRVQQLGLLEEDPTTSGIRLFPRDFQFQQIHGHKSSTGCLGHGALVLQLLSFMLLAGVLVAILV QVSKVPSSLSQEQSEQDAIYQNLTQLKAAVGELSEKSKLQEIYQELTQLKAAVGELPEKSKLQEIYQELT RLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTQLKAAVGELPDQSKQQQIYQELTD LKTAFERLCRHCPKDWTFFQGNCYFMSNSQRNWHDSVTACQEVRAQLVVIKTAEEQNFLQLQTSRSNRFS WMGLSDLNQEGTWQWVDGSPLSPSFQRYWNSGEPNNSGNEDCAEFSGSGWNDNRCDVDNYWICKKPAACF RDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001144909 |
ORF Size | 1059 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001144909.1, NP_001138381.1 |
RefSeq Size | 1838 bp |
RefSeq ORF | 1062 bp |
Locus ID | 10332 |
UniProt ID | Q9H2X3 |
Protein Families | Druggable Genome, Transmembrane |
MW | 40.1 kDa |
Gene Summary | This gene encodes a C-type lectin that functions in cell adhesion and pathogen recognition. This receptor recognizes a wide range of evolutionarily divergent pathogens with a large impact on public health, including tuberculosis mycobacteria, and viruses including Ebola, hepatitis C, HIV-1, influenza A, West Nile virus and the SARS-CoV acute respiratory syndrome coronavirus. The protein is organized into four distinct domains: a C-terminal carbohydrate recognition domain, a flexible tandem-repeat neck domain of variable length, a transmembrane region and an N-terminal cytoplasmic domain involved in internalization. This gene is closely related in terms of both sequence and function to a neighboring gene, CD209 (Gene ID: 30835), also known as DC-SIGN. The two genes differ in viral recognition and expression patterns, with this gene showing high expression in endothelial cells of the liver, lymph node and placenta. Polymorphisms in the tandem repeat neck domain are associated with resistance to SARS infection. [provided by RefSeq, May 2020] |
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