XRN1 (NM_001282859) Human Tagged ORF Clone
CAT#: RC238350
- TrueORF®
XRN1 (myc-DDK-tagged) - Human 5'-3' exoribonuclease 1 (XRN1), transcript variant 4
ORF Plasmid: tGFP
"NM_001282859" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SEP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC238350 representing NM_001282859
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGTCCCCAAGTTTTACAGATGGATCTCAGAGCGGTATCCCTGTCTCAGCGAAGTGGTGAAAGAGC ATCAGATTCCTGAATTTGACAACTTGTACCTGGATATGAATGGAATTATACATCAGTGCTCCCATCCTAA TGATGATGATGTTCACTTTAGAATTTCAGATGATAAAATCTTTACTGATATTTTTCACTACCTGGAGGTG TTGTTTCGCATTATTAAACCCAGGAAAGTGTTCTTTATGGCTGTAGATGGTGTGGCTCCTCGAGCAAAAA TGAACCAGCAGCGTGGGAGGCGTTTTAGGTCAGCAAAGGAGGCAGAAGACAAAATTAAAAAGGCAATAGA GAAGGGAGAAACTCTTCCTACAGAGGCCAGATTTGATTCCAACTGTATCACACCAGGAACTGAATTTATG GCCAGGTTACATGAACATCTGAAGTATTTTGTAAATATGAAAATTTCCACAGACAAGTCATGGCAAGGAG TTACCATCTACTTCTCAGGCCATGAGACTCCTGGAGAAGGAGAGCATAAAATCATGGAATTTATCAGATC CGAGAAAGCAAAGCCAGATCATGATCCAAACACCAGACACTGTCTTTATGGTTTAGATGCTGACTTGATT ATGCTTGGATTAACAAGTCATGAGGCACATTTTTCTCTCTTAAGAGAAGAAGTTCGATTTGGTGGCAAAA AAACACAACGGGTATGTGCTCCAGAAGAAACTACATTTCACCTTCTACACTTGTCTTTAATGAGAGAGTA TATTGACTATGAGTTTTCAGTATTAAAAGAAAAGATCACATTTAAATATGATATTGAAAGGATAATAGAT GATTGGATTTTGATGGGGTTTCTTGTTGGTAATGATTTTATCCCTCATCTACCTCATTTACATATTAATC ATGATGCACTGCCTCTTCTTTATGGAACATATGTTACCATCCTGCCAGAACTTGGGGGTTATATTAATGA AAGTGGGCACCTCAACTTACCTCGATTTGAGAAATACCTTGTGAAACTATCAGATTTTGATCGGGAGCAC TTCAGTGAAGTTTTTGTGGACCTAAAATGGTTTGAAAGCAAAGTTGGTAACAAGTACCTCAATGAAGCAG CAGGTGTCGCAGCAGAAGAAGCCAGGAACTACAAGGAAAAGAAAAAGTTAAAGGGCCAGGAAAATTCTCT GTGTTGGACTGCTTTAGACAAAAATGAAGGCGAAATGATAACTTCTAAGGATAATTTAGAAGATGAGACT GAAGATGATGACCTATTTGAAACTGAGTTTAGACAATATAAAAGAACATATTACATGACGAAGATGGGGG TTGACGTAGTATCTGAGTACGTTTTTGCCAATGCTTTTATTTTAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC238350 representing NM_001282859
Red=Cloning site Green=Tags(s) MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLEV LFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFM ARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLI MLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIERIID DWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSDFDREH FSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKDNLEDET EDDDLFETEFRQYKRTYYMTKMGVDVVSEYVFANAFILK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001282859 |
ORF Size | 1377 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282859.1, NP_001269788.1 |
RefSeq Size | 2152 bp |
RefSeq ORF | 1380 bp |
Locus ID | 54464 |
UniProt ID | Q8IZH2 |
Protein Pathways | RNA degradation |
MW | 54.3 kDa |
Gene Summary | This gene encodes a member of the 5'-3' exonuclease family. The encoded protein may be involved in replication-dependent histone mRNA degradation, and interacts directly with the enhancer of mRNA-decapping protein 4. In addition to mRNA metabolism, a similar protein in yeast has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly. Mutations in this gene are associated with osteosarcoma, suggesting that the encoded protein may also play a role in bone formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
Documents
Product Manuals |
FAQs |
SDS |