XRN1 (NM_001282859) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SEP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238350 representing NM_001282859 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGTCCCCAAGTTTTACAGATGGATCTCAGAGCGGTATCCCTGTCTCAGCGAAGTGGTGAAAGAGC ATCAGATTCCTGAATTTGACAACTTGTACCTGGATATGAATGGAATTATACATCAGTGCTCCCATCCTAA TGATGATGATGTTCACTTTAGAATTTCAGATGATAAAATCTTTACTGATATTTTTCACTACCTGGAGGTG TTGTTTCGCATTATTAAACCCAGGAAAGTGTTCTTTATGGCTGTAGATGGTGTGGCTCCTCGAGCAAAAA TGAACCAGCAGCGTGGGAGGCGTTTTAGGTCAGCAAAGGAGGCAGAAGACAAAATTAAAAAGGCAATAGA GAAGGGAGAAACTCTTCCTACAGAGGCCAGATTTGATTCCAACTGTATCACACCAGGAACTGAATTTATG GCCAGGTTACATGAACATCTGAAGTATTTTGTAAATATGAAAATTTCCACAGACAAGTCATGGCAAGGAG TTACCATCTACTTCTCAGGCCATGAGACTCCTGGAGAAGGAGAGCATAAAATCATGGAATTTATCAGATC CGAGAAAGCAAAGCCAGATCATGATCCAAACACCAGACACTGTCTTTATGGTTTAGATGCTGACTTGATT ATGCTTGGATTAACAAGTCATGAGGCACATTTTTCTCTCTTAAGAGAAGAAGTTCGATTTGGTGGCAAAA AAACACAACGGGTATGTGCTCCAGAAGAAACTACATTTCACCTTCTACACTTGTCTTTAATGAGAGAGTA TATTGACTATGAGTTTTCAGTATTAAAAGAAAAGATCACATTTAAATATGATATTGAAAGGATAATAGAT GATTGGATTTTGATGGGGTTTCTTGTTGGTAATGATTTTATCCCTCATCTACCTCATTTACATATTAATC ATGATGCACTGCCTCTTCTTTATGGAACATATGTTACCATCCTGCCAGAACTTGGGGGTTATATTAATGA AAGTGGGCACCTCAACTTACCTCGATTTGAGAAATACCTTGTGAAACTATCAGATTTTGATCGGGAGCAC TTCAGTGAAGTTTTTGTGGACCTAAAATGGTTTGAAAGCAAAGTTGGTAACAAGTACCTCAATGAAGCAG CAGGTGTCGCAGCAGAAGAAGCCAGGAACTACAAGGAAAAGAAAAAGTTAAAGGGCCAGGAAAATTCTCT GTGTTGGACTGCTTTAGACAAAAATGAAGGCGAAATGATAACTTCTAAGGATAATTTAGAAGATGAGACT GAAGATGATGACCTATTTGAAACTGAGTTTAGACAATATAAAAGAACATATTACATGACGAAGATGGGGG TTGACGTAGTATCTGAGTACGTTTTTGCCAATGCTTTTATTTTAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238350 representing NM_001282859 Red=Cloning site Green=Tags(s) MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLEV LFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFM ARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLI MLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIERIID DWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSDFDREH FSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKDNLEDET EDDDLFETEFRQYKRTYYMTKMGVDVVSEYVFANAFILK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282859
ORF Size	1377 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282859.1, NP_001269788.1
RefSeq Size	2152 bp
RefSeq ORF	1380 bp
Locus ID	54464
UniProt ID	Q8IZH2
Protein Pathways	RNA degradation
MW	54.3 kDa
Gene Summary	This gene encodes a member of the 5'-3' exonuclease family. The encoded protein may be involved in replication-dependent histone mRNA degradation, and interacts directly with the enhancer of mRNA-decapping protein 4. In addition to mRNA metabolism, a similar protein in yeast has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly. Mutations in this gene are associated with osteosarcoma, suggesting that the encoded protein may also play a role in bone formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

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