SPATA6 (NM_001286238) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HASH; SRF-1; SRF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238428 representing NM_001286238 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAAGGTGAAGGCGCTGCAGTGCGCCCTGGCGCTGGAGATCAGCTCAGTAACTTGCCCAGGAGTCG TGCTTAAAGACAAAGAGGACATCTATCTTAGCATCTGTGTGTTTGGCCAATACAAAAAGACACAATGTGT CCCAGCCACTTTTCCCCTGGTCTTCAATGCCAGAATGGTGTTTGAAAAGGTGTTCCCGGACGCAGTAGAT CCTGGAGATGTGGTTACACAGCTTGAATATGATACAGCAGTGTTCGAGTTGATACAGCTAGTTCCACCAG TGGGTGAAACACTGTCTACGTATGACGAAAATACACGAGATTTCATGTTTCCGGGTCCAAACCAAATGTC TGGACACCATGATTCAAACCGCCAGGTTACCATGAGGAGGATTTCTGGCCTTCGAGGAAATGCTCCAAGG CTGGAATTTTCTACGACTTCAGTGATTACTGAATGTCTGATAAGTTCAAGGAAATGCCACACTCAGGATA AATTTATTTACCATTTGGCTCCAGTTGAAAAATCACATGGCAGACTGCAAAACAGAACATCAAGATCACA AAAGAAAAAATCCAAGTCACCTGAGAGAAGTAAATACTGTATAAATGCAAAAAACTACGAACAGCCTACA ATTTCTTCAAAATCACACTCTCCATCTCCCTACACAAAAAGACGCATGTGTGAGCTATCTGAAGACACCA GGCGGCGGCTGGCCCATTTAAATCTGGGACCCTATGAGTTCAAAAAAGAAACAGATAAACCTCCATTTGT GATTAGACATGTTGATCCCCCAAGTCCCAGGGCTGATACTTTATTGGGATCTTCTGGAAGAGACTGTGAA AGAGATGGATGGTCAAGGGTGCACAATGATCATTCTCATCTTGGCTGCTGCCGACCCAAGGATTATAAGG TTATCAGGACACCCCATGGGAGAGACTTCGATGACTCTTTAGAAAAATGTGAAGAGTATTTGAGCCCAAG GTCGTGTAGTAAGCCCCGGCATTCAGCGAGGACCTTGCTAGTCCATTCAGCACCCTCAACAATGCCAAAG CATTCTCCAAGCCCTGTGTTAAATAGAGCTTCTCTCAGGGAAAGATTTCATTCTGATTGGTGTTCACCTT CAAACTGCGATGAGATCCATGACCGGGATGAGAGAGACCTAGAGAAAGATGATGAACTGGAACTGAAAAG AAGTCTTTTATGTAGAGACTCTGCCTATGACAGTGACCCCGAGTATAGCTCATGTCAGCAGCCACGTGGC ACTTTCCATTTGGATGACGGTGAATACTGGTCCAACAGGGCAGCCTCTTATAAGGGAAAATCCCACCGAC CCATCTTTGAGAACAGCATGGACAAGATGTACAGGAACTTATACAAAAAGGCCTGTAGTTCTGCTTCACA TACACAGGAAAGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238428 representing NM_001286238 Red=Cloning site Green=Tags(s) MPKVKALQCALALEISSVTCPGVVLKDKEDIYLSICVFGQYKKTQCVPATFPLVFNARMVFEKVFPDAVD PGDVVTQLEYDTAVFELIQLVPPVGETLSTYDENTRDFMFPGPNQMSGHHDSNRQVTMRRISGLRGNAPR LEFSTTSVITECLISSRKCHTQDKFIYHLAPVEKSHGRLQNRTSRSQKKKSKSPERSKYCINAKNYEQPT ISSKSHSPSPYTKRRMCELSEDTRRRLAHLNLGPYEFKKETDKPPFVIRHVDPPSPRADTLLGSSGRDCE RDGWSRVHNDHSHLGCCRPKDYKVIRTPHGRDFDDSLEKCEEYLSPRSCSKPRHSARTLLVHSAPSTMPK HSPSPVLNRASLRERFHSDWCSPSNCDEIHDRDERDLEKDDELELKRSLLCRDSAYDSDPEYSSCQQPRG TFHLDDGEYWSNRAASYKGKSHRPIFENSMDKMYRNLYKKACSSASHTQESF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286238
ORF Size	1416 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286238.2
RefSeq Size	4976 bp
RefSeq ORF	1419 bp
Locus ID	54558
UniProt ID	Q9NWH7
Protein Families	Secreted Protein
MW	54.5 kDa
Gene Summary	Required for formation of the sperm connecting piece during spermiogenesis. Sperm connecting piece is essential for linking the developing flagellum to the head during late spermiogenesis. May be involved in myosin-based microfilament transport through interaction with myosin subunits.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.