Axotrophin (MARCH7) (NM_001282807) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AXO; AXOT; MARCH-VII; MARCH7; RNF177
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239264 representing NM_001282807 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGGCAACTATGATCATCTTATGTCTTTAGTAACATCTACATCAGCATCAGCATCTGCGTCACCAT TTCAATCTGCATGGTATAGTGAATCTGAGATAACTCAGGGAGCACGCTCAAGATCGCAGAACCAGCAACG GGATCATGATTCAAAAAGACCTAAACTTTCCTGTACAAACTGTACTACCTCAGCTGGGAGAAATGTTGGA AATGGTTTAAACACATTATCAGATTCATCTTGGAGGCATAGTCAAGTTCCTAGATCTTCATCAATGGTAC TTGGATCATTTGGAACAGACTTAATGAGAGAGAGGAGAGATTTGGAGAGAAGAACAGATTCCTCTATTAG TAATCTTATGGATTATAGTCACCGAAGTGGTGATTTCACAACTTCATCATATGTTCAAGACAGAGTTCCT TCATATTCACAAGGAGCAAGACCAAAAGAAAACTCAATGAGCACTTTACAGTTGAATACATCATCCACAA ACCACCAATTGCCTTCTGAACATCAGACCATACTAAGTTCTAGGGACTCCAGAAATTCTTTAAGATCAAA TTTTTCTTCAAGAGAATCAGAATCTTCCCGAAGCAATACGCAGCCTGGATTTTCTTACAGTTCAAGTAGA GATGAAGCCCCAATCATAAGCAATTCAGAAAGGGTTGTTTCATCTCAAAGACCATTTCAAGAATCTTCTG ACAATGAAGGTAGGCGGACAACGAGGAGATTGCTGTCACGCATAGCTTCTAGCATGTCATCTACTTTTTT TTCACGAAGATCTAGTCAGGATTCCTTGAATACAAGATCATTGAATTCTGAAAATTCTTACGTTTCTCCA AGAATCTTGACAGCTTCACAGTCCCGTAGTAATGTACCATCAGCTTCTGAAGTTCCCGATAATAGGGCAT CTGAAGCTTCTCAGGGATTTCGATTTCTTAGGCGAAGATGGGGTTTGTCATCTCTTAGCCACAATCATAG CTCTGAGTCAGATTCAGAAAATTTTAACCAAGAATCTGAAGGTAGAAATACAGGACCATGGTTATCTTCC TCACTTAGAAATAGATGCACACCTTTGTTCTCTAGAAGGAGGCGAGAGGGAAGAGATGAATCTTCAAGGA TACCTACCTCTGATACATCATCTAGATCTCATATTTTTAGAAGAGAATCAAATGAAGTGGTTCACCTTGA AGCACAGAATGATCCTCTTGGAGCTGCTGCCAACAGACCACAAGCATCTGCAGCATCAAGCAGTGCCACA ACAGGTGGCTCTACATCAGATTCGGCTCAAGGTGGAAGAAATACAGGAATATCAGGGATTCTTCCTGGTT CCTTATTCCGGTTTGCAGTCCCTCCAGCACTTGGGAGTAATTTGACCGACAATGTCATGATCACAGTAGA TATTATTCCTTCAGGTTGGAATTCAGCTGATGGTAAAAGTGATAAAACTAAAAGTGCGCCTTCAAGAGAT CCAGAAAGATTGCAGAAAATAAAAGAGAGCCTCCTTTTAGAGGACTCAGAAGAAGAAGAAGGTGACTTAT GTAGAATTTGTCAAATGGCAGCTGCATCATCATCTAATTTGCTGATAGAGCCATGCAAGTGCACAGGAAG TTTGCAGTATGTCCACCAAGACTGTATGAAAAAGTGGTTACAGGCCAAAATTAACTCTGGTTCTTCATTA GAAGCTGTAACCACCTGTGAACTATGTAAAGAGAAGTTGGAGCTTAACCTGGAGGATTTTGATATTCATG AACTACATAGAGCTCATGCAAATGAACAAGCTGAGTATGAGTTTATCAGCTCTGGTCTCTACCTAGTGGT GTTATTGCACTTGTGCGAACAAAGCTTTTCTGATATGATGGGAAATACAAATGAACCAAGCACACGTGTC CGATTTATTAACCTTGCAAGAACTCTTCAGGCACATATGGAAGATCTCGAAACTTCAGAGGATGATTCCG AAGAAGACGGAGACCATAACAGGACATTTGATATTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239264 representing NM_001282807 Red=Cloning site Green=Tags(s) MIGNYDHLMSLVTSTSASASASPFQSAWYSESEITQGARSRSQNQQRDHDSKRPKLSCTNCTTSAGRNVG NGLNTLSDSSWRHSQVPRSSSMVLGSFGTDLMRERRDLERRTDSSISNLMDYSHRSGDFTTSSYVQDRVP SYSQGARPKENSMSTLQLNTSSTNHQLPSEHQTILSSRDSRNSLRSNFSSRESESSRSNTQPGFSYSSSR DEAPIISNSERVVSSQRPFQESSDNEGRRTTRRLLSRIASSMSSTFFSRRSSQDSLNTRSLNSENSYVSP RILTASQSRSNVPSASEVPDNRASEASQGFRFLRRRWGLSSLSHNHSSESDSENFNQESEGRNTGPWLSS SLRNRCTPLFSRRRREGRDESSRIPTSDTSSRSHIFRRESNEVVHLEAQNDPLGAAANRPQASAASSSAT TGGSTSDSAQGGRNTGISGILPGSLFRFAVPPALGSNLTDNVMITVDIIPSGWNSADGKSDKTKSAPSRD PERLQKIKESLLLEDSEEEEGDLCRICQMAAASSSNLLIEPCKCTGSLQYVHQDCMKKWLQAKINSGSSL EAVTTCELCKEKLELNLEDFDIHELHRAHANEQAEYEFISSGLYLVVLLHLCEQSFSDMMGNTNEPSTRV RFINLARTLQAHMEDLETSEDDSEEDGDHNRTFDIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282807
ORF Size	1998 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282807.2
RefSeq Size	3411 bp
RefSeq ORF	2001 bp
Locus ID	64844
UniProt ID	Q9H992
MW	74.2 kDa
Gene Summary	MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Mar 2010]

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