BBS2 (NM_031885) Human Tagged ORF Clone
CAT#: RG204337
- TrueORF®
BBS2 (tGFP-tagged) - Human Bardet-Biedl syndrome 2 (BBS2)
ORF Plasmid: DDK
"NM_031885" in other vectors (6)
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CNY 6,880.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BBS; RP74 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204337 representing NM_031885
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGCCTGTGTTCACCCTGAAACTGCGCCACAAAATCAGCCCCCGAATGGTGGCCATAGGGCGCT ACGACGGGACTCACCCGTGCCTGGCGGCCGCCACCCAAACGGGCAAGGTTTTTATTCATAATCCTCATAC ACGGAACCAGCATGTCAGTGCATCCAGGGTCTTCCAGAGCCCCCTGGAATCTGATGTTTCTCTTCTCAAC ATTAACCAGGCAGTCAGCTGTCTGACTGCAGGCGTATTGAACCCTGAGCTTGGCTATGATGCCCTTTTAG TGGGGACACAGACTAATCTTTTGGCTTATGATGTCTACAATAATTCGGATTTGTTCTACAGAGAGGTAGC AGATGGGGCAAATGTAGTTGTGCTGGGGACATTGGGAGACATTTCTTCCCCTCTTGCGATTATTGGTGGC AATTGTGCTCTGCAAGGTTTCAATCATGAAGGAAGTGATCTCTTTTGGACGGTTACTGGAGACAATGTTA ATTCCTTGGCCTTGTGTGACTTTGATGGTGATGGAAAGAAAGAGCTTCTTGTTGGATCTGAGGATTTTGA TATCCGAGTTTTTAAGGAAGATGAGATTGTGGCAGAAATGACAGAAACAGAGATAGTCACCTCTCTTTGT CCCATGTATGGCAGTCGATTTGGTTATGCCCTTTCCAATGGCACAGTTGGAGTTTATGACAAAACATCCC GATACTGGAGAATTAAATCGAAAAATCATGCCATGAGCATTCATGCTTTTGACCTTAATTCTGATGGAGT GAATGAACTGATAACTGGTTGGTCCAATGGGAAGGTTGATGCTCGAAGTGACCGAACTGGGGAGGTCATC TTTAAGGACAATTTTTCTTCTGCAATTGCCGGTGTGGTAGAGGGAGATTACCGGATGGATGGCCACATAC AGTTAATCTGCTGCTCAGTGGATGGGGAAATCCGGGGCTACCTGCCTGGCACGGCTGAGATGAGGGGCAA CCTCATGGACACCAGTGCAGAGCAGGACCTGATCCGAGAGCTGAGTCAGAAGAAGCAGAATCTGTTGCTG GAACTCCGTAACTATGAGGAAAATGCCAAGGCTGAATTGGCCAGTCCACTGAACGAGGCTGATGGGCATC GGGGCATAATCCCAGCCAATACCAGGCTCCACACCACGCTCTCAGTCAGCCTGGGGAATGAGACCCAAAC TGCTCATACAGAATTACGCATTTCCACTTCTAATGACACCATCATCCGAGCAGTATTGATTTTTGCAGAA GGAATTTTTACAGGTGAAAGCCACGTGGTACATCCCAGCATTCACAACCTCTCCAGTTCCATCTGCATCC CTATTGTGCCTCCCAAAGATGTCCCTGTGGATCTGCACTTGAAGGCATTCGTGGGTTACAGAAGCAGCAC CCAGTTTCATGTATTTGAATCGACAAGACAGCTCCCTCGATTCTCCATGTATGCGCTGACCAGCCTGGAC CCTGCCAGTGAGCCAATCAGTTATGTTAACTTTACCATTGCAGAACGGGCACAGAGGGTTGTTGTATGGC TCGGTCAGAACTTTCTGTTACCAGAAGACACTCACATTCAGAATGCTCCATTTCAAGTGTGTTTCACATC TTTACGGAATGGCGGCCACCTGCATATAAAAATAAAACTTAGTGGAGAGATCACTATAAATACTGATGAT ATTGATTTGGCTGGTGATATCATCCAGTCAATGGCATCATTTTTTGCTATTGAAGACCTTCAAGTAGAAG CGGATTTTCCTGTCTATTTTGAGGAATTACGAAAGGTGCTAGTTAAGGTGGATGAATATCATTCAGTGCA TCAGAAGCTCAGTGCTGATATGGCTGATCATTCTAATTTGATCCGAAGTTTGCTGGTCGGAGCTGAGGAT GCTCGTCTGATGAGGGACATGAAAACAATGAAGAGTCGTTATATGGAACTCTATGACCTTAATAGAGACT TGCTAAATGGATATAAAATTCGCTGTAACAATCACACAGAGCTGTTGGGAAACCTCAAAGCAGTAAATCA AGCAATTCAAAGAGCAGGTCGTCTGCGGGTTGGAAAACCAAAGAACCAGGTGATCACTGCTTGTCGGGAT GCAATTCGAAGCAATAACATCAACACACTGTTCAAAATCATGCGAGTGGGGACAGCTTCTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204337 representing NM_031885
Red=Cloning site Green=Tags(s) MLLPVFTLKLRHKISPRMVAIGRYDGTHPCLAAATQTGKVFIHNPHTRNQHVSASRVFQSPLESDVSLLN INQAVSCLTAGVLNPELGYDALLVGTQTNLLAYDVYNNSDLFYREVADGANVVVLGTLGDISSPLAIIGG NCALQGFNHEGSDLFWTVTGDNVNSLALCDFDGDGKKELLVGSEDFDIRVFKEDEIVAEMTETEIVTSLC PMYGSRFGYALSNGTVGVYDKTSRYWRIKSKNHAMSIHAFDLNSDGVNELITGWSNGKVDARSDRTGEVI FKDNFSSAIAGVVEGDYRMDGHIQLICCSVDGEIRGYLPGTAEMRGNLMDTSAEQDLIRELSQKKQNLLL ELRNYEENAKAELASPLNEADGHRGIIPANTRLHTTLSVSLGNETQTAHTELRISTSNDTIIRAVLIFAE GIFTGESHVVHPSIHNLSSSICIPIVPPKDVPVDLHLKAFVGYRSSTQFHVFESTRQLPRFSMYALTSLD PASEPISYVNFTIAERAQRVVVWLGQNFLLPEDTHIQNAPFQVCFTSLRNGGHLHIKIKLSGEITINTDD IDLAGDIIQSMASFFAIEDLQVEADFPVYFEELRKVLVKVDEYHSVHQKLSADMADHSNLIRSLLVGAED ARLMRDMKTMKSRYMELYDLNRDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKPKNQVITACRD AIRSNNINTLFKIMRVGTASS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031885 |
ORF Size | 2163 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031885.2, NP_114091.2 |
RefSeq Size | 2978 bp |
RefSeq ORF | 2166 bp |
Locus ID | 583 |
UniProt ID | Q9BXC9 |
Domains | FG-GAP |
Protein Families | Druggable Genome |
Gene Summary | This gene is a member of the Bardet-Biedl syndrome (BBS) gene family. Bardet-Biedl syndrome is an autosomal recessive disorder characterized by severe pigmentary retinopathy, obesity, polydactyly, renal malformation and cognitive disability. The proteins encoded by BBS gene family members are structurally diverse and the similar phenotypes exhibited by mutations in BBS gene family members is likely due to their shared roles in cilia formation and function. Many BBS proteins localize to the basal bodies, ciliary axonemes, and pericentriolar regions of cells. BBS proteins may also be involved in intracellular trafficking via microtubule-related transport. The protein encoded by this gene forms a multiprotein BBSome complex with seven other BBS proteins.[provided by RefSeq, Oct 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204337 | BBS2 (Myc-DDK-tagged)-Human Bardet-Biedl syndrome 2 (BBS2) |
CNY 5,280.00 |
|
RC204337L1 | Lenti ORF clone of Human Bardet-Biedl syndrome 2 (BBS2), Myc-DDK-tagged |
CNY 7,680.00 |
|
RC204337L2 | Lenti ORF clone of Human Bardet-Biedl syndrome 2 (BBS2), mGFP tagged |
CNY 7,700.00 |
|
RC204337L3 | Lenti ORF clone of Human Bardet-Biedl syndrome 2 (BBS2), Myc-DDK-tagged |
CNY 7,700.00 |
|
RC204337L4 | Lenti ORF clone of Human Bardet-Biedl syndrome 2 (BBS2), mGFP tagged |
CNY 7,700.00 |
|
SC319657 | BBS2 (untagged)-Human Bardet-Biedl syndrome 2 (BBS2) |
CNY 5,288.00 |