NUP50 (NM_007172) Human Tagged ORF Clone
CAT#: RG209975
- TrueORF®
NUP50 (tGFP-tagged) - Human nucleoporin 50kDa (NUP50), transcript variant 2
ORF Plasmid: DDK
"NM_007172" in other vectors (6)
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CNY 5,256.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | NPAP60; NPAP60L |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209975 representing NM_007172
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAAAGAAATGCCGAGAAGGAACTGACAGATAGGAATTGGGATCAAGAAGATGAAGCTGAAGAGG TGGGAACATTCTCCATGGCCAGTGAGGAAGTCTTGAAGAATAGAGCCATAAAGAAAGCAAAGCGCAGAAA TGTTGGATTTGAATCTGACACTGGAGGAGCCTTTAAAGGTTTTAAAGGTTTGGTGGTACCTTCTGGAGGA GGACGCTTTTCTGGATTTGGTAGTGGCGCTGGAGGGAAGCCTTTGGAAGGACTGTCGAATGGAAACAACA TAACCAGTGCCCCTCCCTTCGCCAGTGCAAAGGCAGCGGCAGATCCCAAGGTAGCCTTTGGTTCTCTTGC TGCAAATGGCCCTACCACCTTGGTTGATAAAGTTTCAAATCCCAAAACTAATGGGGACAGTCAGCAGCCC TCCTCCTCTGGCCTTGCTTCCAGTAAAGCTTGTGTCGGAAATGCCTATCACAAGCAGTTGGCCGCCTTGA ACTGCTCCGTGCGGGATTGGATAGTGAAGCACGTGAATACAAACCCCCTCTGTGATCTGACACCTATCTT TAAAGACTATGAGAAATATTTAGCAAACATTGAACAGCAACACGGGAACAGTGGCAGGAATTCTGAAAGT GAATCTAACAAAGTGGCAGCTGAAACACAGTCTCCTTCCCTTTTTGGCTCAACAAAATTACAGCAAGAGT CAACGTTTTTGTTTCATGGCAACAAAACTGAAGATACACCTGACAAGAAGATGGAGGTGGCATCTGAAAA GAAAACGGACCCATCATCACTAGGAGCGACAAGTGCCTCATTTAATTTCGGCAAGAAAGTTGATAGCTCT GTTTTGGGCTCATTAAGCTCTGTCCCCCTGACTGGATTTTCTTTCTCCCCTGGAAACTCCAGTTTATTTG GCAAAGATACTACCCAGAGTAAACCAGTCTCTTCACCATTTCCCACTAAACCATTGGAGGGCCAAGCAGA AGGTGACAGTGGTGAATGCAAAGGTGGAGATGAAGAAGAGAATGATGAGCCACCCAAAGTAGTAGTTACC GAAGTAAAAGAAGAAGATGCTTTTTACTCCAAAAAGTGTAAACTGTTTTACAAGAAAGACAATGAGTTTA AAGAGAAAGGCATAGGTACTCTGCATTTAAAACCTACAGCAAATCAGAAGACACAGCTTTTGGTGCGGGC AGACACCAATTTAGGCAACATATTGCTGAACGTTCTGATTCCACCCAATATGCCATGTACGCGAACAGGG AAGAATAACGTTCTTATCGTCTGTGTTCCAAATCCACCAATTGACGAGAAGAATGCCACCATGCCAGTCA CCATGTTGATTCGGGTAAAAACCAGCGAGGATGCAGACGAGTTGCACAAAATTTTACTGGAGAAAAAGGA TGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209975 representing NM_007172
Red=Cloning site Green=Tags(s) MAKRNAEKELTDRNWDQEDEAEEVGTFSMASEEVLKNRAIKKAKRRNVGFESDTGGAFKGFKGLVVPSGG GRFSGFGSGAGGKPLEGLSNGNNITSAPPFASAKAAADPKVAFGSLAANGPTTLVDKVSNPKTNGDSQQP SSSGLASSKACVGNAYHKQLAALNCSVRDWIVKHVNTNPLCDLTPIFKDYEKYLANIEQQHGNSGRNSES ESNKVAAETQSPSLFGSTKLQQESTFLFHGNKTEDTPDKKMEVASEKKTDPSSLGATSASFNFGKKVDSS VLGSLSSVPLTGFSFSPGNSSLFGKDTTQSKPVSSPFPTKPLEGQAEGDSGECKGGDEEENDEPPKVVVT EVKEEDAFYSKKCKLFYKKDNEFKEKGIGTLHLKPTANQKTQLLVRADTNLGNILLNVLIPPNMPCTRTG KNNVLIVCVPNPPIDEKNATMPVTMLIRVKTSEDADELHKILLEKKDA TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_007172 |
ORF Size | 1404 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007172.4 |
RefSeq Size | 5225 bp |
RefSeq ORF | 1407 bp |
Locus ID | 10762 |
UniProt ID | Q9UKX7 |
Domains | Ran_BP1 |
Protein Families | Stem cell - Pluripotency |
Gene Summary | The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209975 | NUP50 (Myc-DDK-tagged)-Human nucleoporin 50kDa (NUP50), transcript variant 2 |
CNY 3,656.00 |
|
RC209975L1 | Lenti ORF clone of Human nucleoporin 50kDa (NUP50), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC209975L2 | Lenti ORF clone of Human nucleoporin 50kDa (NUP50), transcript variant 2, mGFP tagged |
CNY 6,056.00 |
|
RC209975L3 | Lenti ORF clone of Human nucleoporin 50kDa (NUP50), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209975L4 | Lenti ORF clone of Human nucleoporin 50kDa (NUP50), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC110197 | NUP50 (untagged)-Human nucleoporin 50kDa (NUP50), transcript variant 2 |
CNY 3,656.00 |