GABRE (NM_004961) Human Tagged ORF Clone
CAT#: RG214063
- TrueORF®
GABRE (tGFP-tagged) - Human gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE)
ORF Plasmid: DDK
"NM_004961" in other vectors (6)
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CNY 5,368.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG214063 representing NM_004961
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTCCAAAGTTCTTCCAGTCCTCCTAGGCATCTTATTGATCCTCCAGTCGAGGGTCGAGGGACCTC AGACTGAATCAAAGAATGAAGCCTCTTCCCGTGATGTTGTCTATGGCCCCCAGCCCCAGCCTCTGGAAAA TCAGCTCCTCTCTGAGGAAACAAAGTCAACTGAGACTGAGACTGGGAGCAGAGTTGGCAAACTGCCAGAA GCCTCTCGCATCCTGAACACTATCCTGAGTAATTATGACCACAAACTGCGCCCTGGCATTGGAGAGAAGC CCACTGTGGTCACTGTTGAGATCTCCGTCAACAGCCTTGGTCCTCTCTCTATCCTAGACATGGAATACAC CATTGACATCATCTTCTCCCAGACCTGGTACGACGAACGCCTCTGTTACAACGACACCTTTGAGTCTCTT GTTCTGAATGGCAATGTGGTGAGCCAGCTATGGATCCCGGACACCTTTTTTAGGAATTCTAAGAGGACCC ACGAGCATGAGATCACCATGCCCAACCAGATGGTCCGCATCTACAAGGATGGCAAGGTGTTGTACACAAT TAGGATGACCATTGATGCCGGATGCTCACTCCACATGCTCAGATTTCCAATGGATTCTCACTCTTGCCCT CTATCTTTCTCTAGCTTTTCCTATCCTGAGAATGAGATGATCTACAAGTGGGAAAATTTCAAGCTTGAAA TCAATGAGAAGAACTCCTGGAAGCTCTTCCAGTTTGATTTTACAGGAGTGAGCAACAAAACTGAAATAAT CACAACCCCAGTTGGTGACTTCATGGTCATGACGATTTTCTTCAATGTGAGCAGGCGGTTTGGCTATGTT GCCTTTCAAAACTATGTCCCTTCTTCCGTGACCACGATGCTCTCCTGGGTTTCCTTTTGGATCAAGACAG AGTCTGCTCCAGCCCGGACCTCTCTAGGGATCACCTCTGTTCTGACCATGACCACGTTGGGCACCTTTTC TCGTAAGAATTTCCCGCGTGTCTCCTATATCACAGCCTTGGATTTCTATATCGCCATCTGCTTCGTCTTC TGCTTCTGCGCTCTGTTGGAGTTTGCTGTGCTCAACTTCCTGATCTACAACCAGACAAAAGCCCATGCTT CTCCTAAACTCCGCCATCCTCGTATCAATAGCCGTGCCCATGCCCGTACCCGTGCACGTTCCCGAGCCTG TGCCCGCCAACATCAGGAAGCTTTTGTGTGCCAGATTGTCACCACTGAGGGAAGTGATGGAGAGGAGCGC CCGTCTTGCTCAGCCCAGCAGCCCCCTAGCCCAGGTAGCCCTGAGGGTCCCCGCAGCCTCTGCTCCAAGC TGGCCTGCTGTGAGTGGTGCAAGCGTTTTAAGAAGTACTTCTGCATGGTCCCCGATTGTGAGGGCAGTAC CTGGCAGCAGGGCCGCCTCTGCATCCATGTCTACCGCCTGGATAACTACTCGAGAGTTGTTTTCCCAGTG ACTTTCTTCTTCTTCAATGTGCTCTACTGGCTTGTTTGCCTTAACTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214063 representing NM_004961
Red=Cloning site Green=Tags(s) MLSKVLPVLLGILLILQSRVEGPQTESKNEASSRDVVYGPQPQPLENQLLSEETKSTETETGSRVGKLPE ASRILNTILSNYDHKLRPGIGEKPTVVTVEISVNSLGPLSILDMEYTIDIIFSQTWYDERLCYNDTFESL VLNGNVVSQLWIPDTFFRNSKRTHEHEITMPNQMVRIYKDGKVLYTIRMTIDAGCSLHMLRFPMDSHSCP LSFSSFSYPENEMIYKWENFKLEINEKNSWKLFQFDFTGVSNKTEIITTPVGDFMVMTIFFNVSRRFGYV AFQNYVPSSVTTMLSWVSFWIKTESAPARTSLGITSVLTMTTLGTFSRKNFPRVSYITALDFYIAICFVF CFCALLEFAVLNFLIYNQTKAHASPKLRHPRINSRAHARTRARSRACARQHQEAFVCQIVTTEGSDGEER PSCSAQQPPSPGSPEGPRSLCSKLACCEWCKRFKKYFCMVPDCEGSTWQQGRLCIHVYRLDNYSRVVFPV TFFFFNVLYWLVCLNL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004961 |
ORF Size | 1518 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004961.4 |
RefSeq Size | 3167 bp |
RefSeq ORF | 1521 bp |
Locus ID | 2564 |
UniProt ID | P78334 |
Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
Gene Summary | The product of this gene belongs to the ligand-gated ionic channel (TC 1.A.9) family. It encodes the gamma-aminobutyric acid (GABA) A receptor which is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes an epsilon subunit. It is mapped to chromosome Xq28 in a cluster comprised of genes encoding alpha 3, beta 4 and theta subunits of the same receptor. Alternatively spliced transcript variants have been identified, but only one is thought to encode a protein. [provided by RefSeq, Oct 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214063 | GABRE (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE) |
CNY 3,768.00 |
|
RC214063L1 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE), Myc-DDK-tagged |
CNY 6,168.00 |
|
RC214063L2 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE), mGFP tagged |
CNY 6,370.00 |
|
RC214063L3 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE), Myc-DDK-tagged |
CNY 6,370.00 |
|
RC214063L4 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE), mGFP tagged |
CNY 6,370.00 |
|
SC310309 | GABRE (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE) |
CNY 3,776.00 |