DAP Kinase 1 (DAPK1) (NM_004938) Human Tagged ORF Clone
CAT#: RG215423
- TrueORF®
DAPK1 (tGFP-tagged) - Human death-associated protein kinase 1 (DAPK1)
ORF Plasmid: DDK
"NM_004938" in other vectors (7)
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CNY 11,384.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DAPK; ROCO3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG215423 representing NM_004938
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGTGTTCAGGCAGGAAAACGTGGATGATTACTACGACACCGGCGAGGAACTTGGCAGTGGACAGT TTGCGGTTGTGAAGAAATGCCGTGAGAAAAGCACCGGCCTCCAGTATGCCGCCAAATTCATCAAGAAAAG GAGGACTAAGTCCAGCCGGCGGGGTGTGAGCCGCGAGGACATCGAGCGGGAGGTCAGCATCCTGAAGGAG ATCCAGCACCCCAATGTCATCACCCTGCACGAGGTCTATGAGAACAAGACGGACGTCATCCTGATCTTGG AACTCGTTGCAGGTGGCGAGCTGTTTGACTTCTTAGCTGAAAAGGAATCTTTAACTGAAGAGGAAGCAAC TGAATTTCTCAAACAAATTCTTAATGGTGTTTACTACCTGCACTCCCTTCAAATCGCCCACTTTGATCTT AAGCCTGAGAACATAATGCTTTTGGATAGAAATGTCCCCAAACCTCGGATCAAGATCATTGACTTTGGGT TGGCCCATAAAATTGACTTTGGAAATGAATTTAAAAACATATTTGGGACTCCAGAGTTTGTCGCTCCTGA GATAGTCAACTATGAACCTCTTGGTCTTGAGGCAGATATGTGGAGTATCGGGGTAATAACCTATATCCTC CTAAGTGGGGCCTCCCCATTTCTTGGAGACACTAAGCAAGAAACGTTAGCAAATGTATCCGCTGTCAACT ACGAATTTGAGGATGAATACTTCAGTAATACCAGTGCCCTAGCCAAAGATTTCATAAGAAGACTTCTGGT CAAGGATCCAAAGAAGAGAATGACAATTCAAGATAGTTTGCAGCATCCCTGGATCAAGCCTAAAGATACA CAACAGGCACTTAGTAGAAAAGCATCAGCAGTAAACATGGAGAAATTCAAGAAGTTTGCAGCCCGGAAAA AATGGAAACAATCCGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGGTCATTCCTGTCCAGAAGTAA CATGAGTGTTGCCAGAAGCGATGATACTCTGGATGAGGAAGACTCCTTTGTGATGAAAGCCATCATCCAT GCCATCAACGATGACAATGTCCCAGGCCTGCAGCACCTTCTGGGCTCATTATCCAACTATGATGTTAACC AACCCAACAAGCACGGGACACCTCCATTACTCATTGCTGCTGGCTGTGGGAATATTCAAATACTACAGTT GCTCATTAAAAGAGGCTCGAGAATCGATGTCCAGGATAAGGGCGGGTCCAATGCCGTCTACTGGGCTGCT CGGCATGGCCACGTCGATACCTTGAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGT CTGGAGAGATGGCCCTCCACGTGGCAGCTCGCTATGGCCATGCTGACGTGGCTCAGTTACTGTGCAGCTT CGGCTCAAATCCCAATATCCAGGACAAGGAAGAAGAAACCCCCCTGCACTGTGCTGCTTGGCACGGCTAT TACTCTGTGGCCAAAGCCCTTTGTGAAGCCGGCTGTAACGTGAACATCAAGAACCGAGAAGGAGAGACGC CCCTCCTGACAGCCTCTGCCAGGGGCTACCACGACATCGTGGAGTGTCTGGCCGAACATGGAGCCGATCT TAATGCTTGCGACAAGGACGGACACATTGCCCTTCATCTGGCTGTAAGACGGTGTCAGATGGAGGTAATC AAGACTCTCCTCAGCCAAGGGTGTTTCGTCGATTATCAAGACAGGCACGGCAATACTCCCCTCCATGTGG CATGTAAAGATGGCAACATGCCTATCGTGGTGGCCCTCTGTGAAGCAAACTGCAATTTGGACATCTCCAA CAAGTATGGGCGAACGCCTCTGCACCTTGCGGCCAACAACGGAATCCTAGACGTGGTCCGGTATCTCTGT CTGATGGGAGCCAGCGTTGAGGCGCTGACCACGGACGGAAAGACGGCAGAAGATCTTGCTAGATCGGAAC AGCACGAGCACGTAGCAGGTCTCCTTGCAAGACTTCGAAAGGATACGCACCGAGGACTCTTCATCCAGCA GCTCCGACCCACACAGAACCTGCAGCCAAGAATTAAGCTCAAGCTGTTTGGCCACTCGGGATCCGGGAAA ACCACCCTTGTAGAATCTCTCAAGTGTGGGCTGCTGAGGAGCTTTTTCAGAAGGCGTCGGCCCAGACTGT CTTCCACCAACTCCAGCAGGTTCCCACCTTCACCCCTGGCTTCTAAGCCCACAGTCTCAGTGAGCATCAA CAACCTGTACCCAGGCTGCGAGAACGTGAGTGTGAGGAGCCGCAGCATGATGTTCGAGCCGGGTCTTACC AAAGGGATGCTGGAGGTGTTTGTGGCCCCGACCCACCACCCGCACTGCTCGGCCGATGACCAGTCCACCA AGGCCATCGACATCCAGAACGCTTATTTGAATGGAGTTGGCGATTTCAGCGTGTGGGAGTTCTCTGGAAA TCCTGTGTATTTCTGCTGTTATGACTATTTTGCTGCAAATGATCCCACGTCAATCCATGTTGTTGTCTTT AGTCTAGAAGAGCCCTATGAGATCCAGCTGAACCAAGTGATTTTCTGGCTCAGTTTCCTGAAGTCCCTTG TCCCAGTTGAAGAACCCATAGCCTTCGGTGGCAAGCTGAAGAACCCACTCCAAGTTGTCCTGGTGGCCAC CCACGCTGACATCATGAATGTTCCTCGACCGGCTGGAGGCGAGTTTGGATATGACAAAGACACATCGTTG CTGAAAGAGATTAGGAACAGGTTTGGAAATGATCTTCACATTTCAAATAAGCTGTTTGTTCTGGATGCTG GGGCTTCTGGGTCAAAGGACATGAAGGTACTTCGAAATCATCTGCAAGAAATACGAAGCCAGATTGTTTC GGTCTGTCCTCCCATGACTCACCTGTGTGAGAAAATCATCTCCACGCTGCCTTCCTGGAGGAAGCTCAAT GGACCCAACCAGCTGATGTCGCTGCAGCAGTTTGTGTACGACGTGCAGGACCAGCTGAACCCCCTGGCCA GCGAGGAGGACCTCAGGCGCATTGCTCAGCAGCTCCACAGCACAGGCGAGATCAACATCATGCAAAGTGA AACAGTTCAGGACGTGCTGCTCCTGGACCCCCGCTGGCTCTGCACAAACGTCCTGGGGAAGTTGCTGTCC GTGGAGACCCCACGGGCGCTGCACCACTACCGGGGCCGCTACACCGTGGAGGACATCCAGCGCCTGGTGC CCGACAGCGACGTGGAGGAGCTGCTGCAGATCCTCGATGCCATGGACATCTGCGCCCGGGACCTGAGCAG CGGGACCATGGTGGACGTCCCAGCCCTGATCAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAG GACGAGGTGATGGTGTATGGTGGCGTGCGCATCGTGCCCGTGGAACACCTCACCCCCTTCCCATGTGGCA TCTTTCACAAGGTCCAGGTGAACCTGTGCCGGTGGATCCACCAGCAAAGCACAGAGGGCGACGCGGACAT CCGCCTGTGGGTGAATGGCTGCAAGCTGGCCAACCGTGGGGCCGAGCTGCTGGTGCTGCTGGTCAACCAC GGCCAGGGCATTGAGGTCCAGGTCCGTGGCCTGGAGACGGAGAAGATCAAGTGCTGCCTGCTGCTGGACT CGGTGTGCAGCACCATTGAGAACGTCATGGCCACCACGCTGCCAGGGCTCCTGACCGTGAAGCATTACCT GAGCCCCCAGCAGCTGCGGGAGCACCATGAGCCCGTCATGATCTACCAGCCACGGGACTTCTTCCGGGCA CAGACTCTGAAGGAAACCTCACTGACCAACACCATGGGGGGGTACAAGGAAAGCTTCAGCAGCATCATGT GCTTCGGGTGTCACGACGTCTACTCACAGGCCAGCCTCGGCATGGACATCCATGCATCAGACCTGAACCT CCTCACTCGGAGGAAACTGAGTCGCCTGCTGGACCCGCCCGACCCCCTGGGGAAGGACTGGTGCCTTCTC GCCATGAACTTAGGCCTCCCTGACCTCGTGGCAAAGTACAACACCAATAACGGGGCTCCCAAGGATTTCC TCCCCAGCCCCCTCCACGCCCTGCTGCGGGAATGGACCACCTACCCTGAGAGCACAGTGGGCACCCTCAT GTCCAAACTGAGGGAGCTGGGTCGCCGGGATGCCGCAGACTTTTTGCTGAAGGCATCCTCTGTGTTCAAA ATCAACCTGGATGGCAATGGCCAGGAGGCCTATGCCTCGAGCTGCAACAGCGGCACCTCTTACAATTCCA TTAGCTCTGTTGTATCCCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215423 representing NM_004938
Red=Cloning site Green=Tags(s) MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKE IQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDL KPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYIL LSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDT QQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIH AINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDVQDKGGSNAVYWAA RHGHVDTLKFLSENKCPLDVKDKSGEMALHVAARYGHADVAQLLCSFGSNPNIQDKEEETPLHCAAWHGY YSVAKALCEAGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNACDKDGHIALHLAVRRCQMEVI KTLLSQGCFVDYQDRHGNTPLHVACKDGNMPIVVALCEANCNLDISNKYGRTPLHLAANNGILDVVRYLC LMGASVEALTTDGKTAEDLARSEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGK TTLVESLKCGLLRSFFRRRRPRLSSTNSSRFPPSPLASKPTVSVSINNLYPGCENVSVRSRSMMFEPGLT KGMLEVFVAPTHHPHCSADDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVVVF SLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLQVVLVATHADIMNVPRPAGGEFGYDKDTSL LKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLCEKIISTLPSWRKLN GPNQLMSLQQFVYDVQDQLNPLASEEDLRRIAQQLHSTGEINIMQSETVQDVLLLDPRWLCTNVLGKLLS VETPRALHHYRGRYTVEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDVPALIKTDNLHRSWADEE DEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVNGCKLANRGAELLVLLVNH GQGIEVQVRGLETEKIKCCLLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRA QTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPPDPLGKDWCLL AMNLGLPDLVAKYNTNNGAPKDFLPSPLHALLREWTTYPESTVGTLMSKLRELGRRDAADFLLKASSVFK INLDGNGQEAYASSCNSGTSYNSISSVVSR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004938 |
ORF Size | 4290 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004938.1, NP_004929.1 |
RefSeq Size | 5910 bp |
RefSeq ORF | 4293 bp |
Locus ID | 1612 |
UniProt ID | P53355 |
Domains | DEATH, pkinase, TyrKc, ANK, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Bladder cancer, Pathways in cancer |
Gene Summary | Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215423 | DAPK1 (Myc-DDK-tagged)-Human death-associated protein kinase 1 (DAPK1) |
CNY 9,784.00 |
|
RC215423L1 | Lenti ORF clone of Human death-associated protein kinase 1 (DAPK1), Myc-DDK-tagged |
CNY 12,184.00 |
|
RC215423L2 | Lenti ORF clone of Human death-associated protein kinase 1 (DAPK1), mGFP tagged |
CNY 12,184.00 |
|
RC215423L3 | Lenti ORF clone of Human death-associated protein kinase 1 (DAPK1), Myc-DDK-tagged |
CNY 12,184.00 |
|
RC215423L4 | Lenti ORF clone of Human death-associated protein kinase 1 (DAPK1), mGFP tagged |
CNY 12,184.00 |
|
SC125504 | DAPK1 (untagged)-Human death-associated protein kinase 1 (DAPK1) |
CNY 9,800.00 |
|
SC323458 | DAPK1 (untagged)-Kinase deficient mutant (K42M) of Human death-associated protein kinase 1 (DAPK1) |
CNY 9,800.00 |
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