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CDC42 binding protein kinase alpha (CDC42BPA) (NM_014826) Human Tagged ORF Clone
CAT＃: RG222587
- TrueORF®
CDC42BPA (tGFP-tagged) - Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A
ORF Plasmid: DDK
"NM_014826" in other vectors (5)
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CNY 13,392.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MRCK; MRCKA; PK428 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222587 representing NM_014826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTTTAAAAGACAAATTGGAAAAAACCAGAAGAGAAAGTCAAAGTGAAAGGGAGGAATTTGAAAGTG AGTTCAAACAACAATATGAACGAGAAAAAGTGTTGTTAACTGAAGAAAATAAAAAGCTGACGAGTGAACT TGATAAGCTTACTACTTTGTATGAGAACTTAAGTATACACAACCAGCAGTTAGAAGAAGAGGTTAAAGAT CTAGCAGACAAGAAAGAATCAGTTGCACATTGGGAAGCCCAAATCACAGAAATAATTCAGTGGGTCAGCG ATGAAAAGGATGCACGAGGGTATCTTCAGGCCTTAGCTTCTAAAATGACTGAAGAATTGGAGGCATTAAG AAATTCCAGCTTGGGTACACGAGCAACAGATATGCCCTGGAAAATGCGTCGTTTTGCGAAACTGGATATG TCAGCTAGACTGGAGTTGCAGTCGGCTCTGGATGCAGAAATAAGAGCCAAACAGGCCATCCAAGAAGAGT TGAATAAAGTTAAAGCATCTAATATCATAACAGAATGTAAACTAAAAGATTCAGAGAAGAAGAACTTGGA ACTACTCTCAGAAATCGAACAGCTGATAAAGGACACTGAAGAGCTTAGATCTGAAAAGGGTATAGAGCAC CAAGACTCACAGCATTCTTTCTTGGCATTTTTGAATACGCCTACCGATGCTCTGGATCAATTTGAAACTG ATCCCGTTGAGAACACATATGTATGGAACCCGAGCGTCAAGTTTCACATCCAGTCACGGTCCACATCTCC TTCCACATCTAGTGAAGCTGAGCCAGTTAAGACTGTAGACTCCACTCCACTTTCAGTTCACACACCAACC TTAAGGAAAAAAGGATGTCCTGGTTCAACTGGCTTTCCACCTAAGCGCAAGACTCACCAGTTTTTTGTAA AATCTTTTACTACTCCTACCAAGTGTCATCAGTGTACCTCCTTGATGGTGGGTTTAATAAGACAGGGCTG TTCATGTGAAGTGTGTGGATTCTCATGCCATATAACTTGTGTAAACAAAGCTCCAACCACTTGTCCAGTT CCTCCTGAACAGACAAAAGGTCCCCTGGGTATAGATCCTCAGAAAGGAATAGGAACAGCATATGAAGGTC ATGTCAGGATTCCTAAGCCAGCTGGAGTGAAGAAAGGGTGGCAGAGAGCACTGGCTATAGTGTGTGACTT CAAACTCTTTCTGTACGATATTGCTGAAGGAAAAGCATCTCAGCCCAGTGTTGTCATTAGTCAAGTGATT GACATGAGGGATGAAGAATTTTCTGTGAGTTCAGTCTTGGCTTCTGATGTTATCCATGCAAGTCGGAAAG ATATACCCTGTATATTTAGGGTCACAGCTTCCCAGCTCTCAGCATCTAATAACAAATGTTCAATCCTGAT GCTAGCAGACACTGAGAATGAGAAGAATAAGTGGGTGGGAGTGCTGAGTGAATTGCACAAGATTTTGAAG AAAAACAAATTCAGAGACCGCTCAGTCTATGTTCCCAAAGAGGCTTATGACAGCACTCTACCCCTCATTA AAACAACCCAGGCAGCCGCAATCATAGATCATGAAAGAATTGCTTTGGGAAACGAAGAAGGGTTATTTGT TGTACATGTCACCAAAGATGAAATTATTAGAGTTGGTGACAATAAGAAGATTCATCAGATTGAACTCATT CCAAATGATCAGCTTGTTGCTGTGATCTCAGGACGAAATCGTCATGTACGACTTTTTCCTATGTCAGCAT TGGATGGGCGAGAGACCGATTTTTACAAGCTGTCAGAAACTAAAGGGTGTCAAACCATAACTTCTGGAAA GGTGCGCCATGGAGCTCTCACATGCCTGTGTGTGGCTATGAAAAGGCAGGTCCTCTGTTATGAACTATTT CAGAGCAAGACCCGTCACAGAAAATTTAAGGAAATTCAAGTCCCATATAATGTCCAGTGGATGGCAATCT TCAGTGAACAACTCTGTGTGGGATTCCAGTCAGGATTTCTAAGATACCCCTTGAATGGAGAAGGAAATCC ATACAGTATGCTCCATTCAAATGACCATACACTATCATTTATTGCACATCAACCAATGGATGCTATCTGC GCAGTTGAGATCTCCAGTAAAGAATATCTGCTGTGTTTTAACAGCATTGGGATATACACTGACTGCCAGG GCCGAAGATCTAGACAACAGGAATTGATGTGGCCAGCAAATCCTTCCTCTTGTTGTTACAATGCACCATA TCTCTCGGTGTACAGTGAAAATGCAGTTGATATCTTTGATGTGAACTCCATGGAATGGATTCAGACTCTT CCTCTCAAAAAGGTTCGACCCTTAAACAATGAAGGATCATTAAATCTTTTAGGGTTGGAGACCATTAGAT TAATATATTTCAAAAATAAGATGGCAGAAGGGGACGAACTGGTAGTACCTGAAACATCAGATAATAGTCG GAAACAAATGGTTAGAAACATTAACAATAAGCGGCGTTATTCCTTCAGAGTCCCAGAAGAGGAAAGGATG CAGCAGAGGAGGGAAATGCTACGAGATCCAGAAATGAGAAATAAATTAATTTCTAATCCAACTAATTTTA ATCACATAGCACACATGGGTCCTGGAGATGGAATACAGATCCTGAAAGATCTGCCCATGAACCCTCGGCC TCAGGAAAGTCGGACAGTATTCAGTGGCTCAGTCAGTATTCCATCTATCACCAAATCCCGCCCTGAGCCA GGCCGCTCCATGAGTGCTAGCAGTGGCTTGTCAGCAAGGTCATCCGCACAGAATGGCAGCGCATTAAAGA GGGAATTCTCTGGAGGAAGCTACAGTGCCAAGCGGCAGCCCATGCCCTCCCCGTCAGAGGGCTCTTTGTC CTCCGGAGGCATGGACCAAGGAAGTGATGCCCCAGCGAGGGACTTTGACGGAGAGGACTCTGACTCTCCG AGGCATTCCACAGCTTCCAACAGTTCCAACCTAAGCAGCCCCCCAAGCCCAGTTTCACCCCGAAAAACCA AGAGCCTCTCCCTGGAGAGCACTGACCGCGGGAGCTGGGACCCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222587 representing NM_014826
Red=Cloning site Green=Tags(s) MILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKD LADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDM SARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEH QDSQHSFLAFLNTPTDALDQFETDPVENTYVWNPSVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPT LRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPV PPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVI DMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILK KNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELI PNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELF QSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAIC AVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTL PLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERM QQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEP GRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSP RHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014826 |
| ORF Size | 3054 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014826.3, NP_055641.3 |
| RefSeq Size | 7776 bp |
| RefSeq ORF | 4917 bp |
| Locus ID | 8476 |
| UniProt ID | Q5VT25 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described. [provided by RefSeq, Sep 2018] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222587 | CDC42BPA (Myc-DDK-tagged)-Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A |
CNY 11,792.00 |
|
| RC222587L3 | Lenti-ORF clone of CDC42BPA (Myc-DDK-tagged)-Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A |
CNY 19,380.00 |
|
| RC222587L4 | Lenti-ORF clone of CDC42BPA (mGFP-tagged)-Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A |
CNY 19,380.00 |
|
| SC114791 | CDC42BPA (untagged)-Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A |
CNY 11,216.00 |
|
| SC323610 | CDC42BPA (untagged)-Kinase deficient mutant (K106M) of Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A |
CNY 11,216.00 |
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