NT5C1B (NM_033253) Human Tagged ORF Clone
CAT#: RG224105
- TrueORF®
NT5C1B (tGFP-tagged) - Human 5'-nucleotidase, cytosolic IB (NT5C1B), transcript variant 2
ORF Plasmid: DDK
"NM_033253" in other vectors (4)
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CNY 5,696.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AIRP; CN-IB; CN1B |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG224105 representing NM_033253
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGTCCTCAAAAGAGAGTCTAGAAG CAGAAAAAAGAAAGGAATCTGACAAAACAGGAGTTCGTCTGAGCAATCAGGGATCACAAGAATCATCACT GCGGAAGACAGACTCTCGAGGGTACCTTGTGCGCAGTCAATGGTCTAGAATATCCCGGAGCCCATCCACC AAGGCTCCATCCATAGATGAGCCTAGAAGCAGGAACACCAGTGCTAAGCTCCCCAGCAGCTCCACGAGCT CCCGGACTCCATCCACCTCCCCAAGCCTGCATGACTCCTCACCGCCGCCGCTGTCCGGGCAGCCCTCGCT CCAGCCACCCGCGTCGCCCCAGCTGCCCCGGTCGCTGGACTCGCGGCCTCCCACGCCCCCAGAGCCCGAT CCTGGCTCCCGGCGCAGCACCAAAATGCAAGAGAATCCGGAGGCCTGGGCCCAAGGCATCGTGCGGGAAA TCCGCCAGACCCGGGACTCGCAGCCGCTGGAATATTCGCGCACGTCCCCCACCGAGTGGAAGTCCTCCAG CCAGCGCAGGGGGATCTACCCCGCCTCCACCCAGCTGGACCGCAACTCTCTGTCCGAGCAGCAGCAGCAG CAGCGGGAGGACGAGGACGACTACGAGGCTGCCTACTGGGCATCCATGAGGTCGTTCTACGAGAAGAACC CGAGCTGCTCGCGCCCCTGGCCGCCCAAACCCAAGAACGCCATCACCATTGCTCTCTCATCCTGCGCGCT CTTCAACATGGTGGACGGCAGGAAAATCTACGAGCAAGAGGGTCTGGAAAAGTACATGGAGTATCAGCTC ACCAATGAGAACGTCATCCTGACCCCGGGCCCGGCGTTCCGCTTCGTCAAGGCACTACAGTATGTCAATG CTAGACTCCGTGATCTATATCCTGATGAACAGGACTTATTTGATATTGTACTGATGACTAATAACCATGC CCAAGTGGGAGTGCGGCTTATAAACAGCGTCAATCACTACGGCTTACTGATTGACCGCTTCTGTCTGACC GGGGGAAAAGACCCCATTGGCTATTTGAAGGCATATCTTACCAACTTGTATATTGCTGCAGATTCTGAAA AAGTGCAAGAGGCAATACAAGAAGGTATTGCCTCTGCGACAATGTTTGATGGAGCCAAAGACATGGCTTA CTGTGACACTCAGCTCCGTGTAGCCTTTGATGGGGATGCTGTCCTCTTCTCTGATGAGTCTGAACATTTT ACCAAGGAGCATGGGCTCGACAAATTCTTCCAGTATGATACATTATGTGAAAGTAAGCCTCTTGCTCAGG GTCCCCTAAAAGGCTTTCTGGAAGATTTAGGCAGACTGCAAAAGAAGTTCTATGCCAAAAATGAACGGTT ACTTTGTCCTATCAGGACCTACCTGGTTACAGCTAGGAGTGCAGCCAGTTCAGGCGCCCGTGTGCTGAAG ACCCTTCGACGCTGGGGTCTAGAGATAGACGAAGCTCTTTTCCTTGCTGGAGCCCCCAAAAGTCCCATCT TGGTGAAGATCCGGCCCCACATCTTCTTTGATGACCACATGTTCCACATTGAAGGGGCACAGAGGTTAGG TTCCATCGCAGCTTATGGCTTTAATAAAAAATTCAGTAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224105 representing NM_033253
Red=Cloning site Green=Tags(s) MSQTSLKQKKNEPGMRSSKESLEAEKRKESDKTGVRLSNQGSQESSLRKTDSRGYLVRSQWSRISRSPST KAPSIDEPRSRNTSAKLPSSSTSSRTPSTSPSLHDSSPPPLSGQPSLQPPASPQLPRSLDSRPPTPPEPD PGSRRSTKMQENPEAWAQGIVREIRQTRDSQPLEYSRTSPTEWKSSSQRRGIYPASTQLDRNSLSEQQQQ QREDEDDYEAAYWASMRSFYEKNPSCSRPWPPKPKNAITIALSSCALFNMVDGRKIYEQEGLEKYMEYQL TNENVILTPGPAFRFVKALQYVNARLRDLYPDEQDLFDIVLMTNNHAQVGVRLINSVNHYGLLIDRFCLT GGKDPIGYLKAYLTNLYIAADSEKVQEAIQEGIASATMFDGAKDMAYCDTQLRVAFDGDAVLFSDESEHF TKEHGLDKFFQYDTLCESKPLAQGPLKGFLEDLGRLQKKFYAKNERLLCPIRTYLVTARSAASSGARVLK TLRRWGLEIDEALFLAGAPKSPILVKIRPHIFFDDHMFHIEGAQRLGSIAAYGFNKKFSS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_033253 |
ORF Size | 1650 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033253.4 |
RefSeq Size | 2684 bp |
RefSeq ORF | 1653 bp |
Locus ID | 93034 |
UniProt ID | Q96P26 |
Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
Gene Summary | Cytosolic 5-prime nucleotidases, such as NT5C1B, catalyze production of adenosine, which regulates diverse physiologic processes (Sala-Newby and Newby, 2001 [PubMed 11690631]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224105 | NT5C1B (Myc-DDK-tagged)-Human 5'-nucleotidase, cytosolic IB (NT5C1B), transcript variant 2 |
CNY 4,096.00 |
|
RC224105L3 | Lenti ORF clone of Human 5'-nucleotidase, cytosolic IB (NT5C1B), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC224105L4 | Lenti ORF clone of Human 5'-nucleotidase, cytosolic IB (NT5C1B), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC305600 | NT5C1B (untagged)-Human 5'-nucleotidase, cytosolic IB (NT5C1B), transcript variant 2 |
CNY 4,104.00 |