NBL1 (NM_001204084) Human Tagged ORF Clone
CAT#: RG233379
- TrueORF®
NBL1 (tGFP-tagged) - Homo sapiens neuroblastoma 1, DAN family BMP antagonist (NBL1), transcript variant 3
ORF Plasmid: DDK
"NM_001204084" in other vectors (2)
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CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | D1S1733E; DAN; DAND1; NB; NO3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG233379 representing NM_001204084
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTTCGGGTCCTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACA AGCTGGCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGCCACAG CGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGCTACAGCGTCCCCAAC ACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGCATGCCAGCCCAGTCCATGTGGGAGA TTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTGCCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCA CTGTAGCTGCCAGGCCTGCGGCAAGGAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGAC GGGCCGGGATCCCAGCCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTG AGCCCGAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233379 representing NM_001204084
Red=Cloning site Green=Tags(s) MMLRVLVGAVLPAMLLAAPPPINKLALFPDKSAWCEAKNITQIVGHSGCEAKSIQNRACLGQCFSYSVPN TFPQSTESLVHCDSCMPAQSMWEIVTLECPGHEEVPRVDKLVEKILHCSCQACGKEPSHEGLSVYVQGED GPGSQPGTHPHPHPHPHPGGQTPEPEDPPGAPHTEEEGAED TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001204084 |
ORF Size | 543 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001204084.2, NP_001191013.1 |
RefSeq Size | 2151 bp |
RefSeq ORF | 546 bp |
Locus ID | 4681 |
UniProt ID | P41271 |
Protein Families | Secreted Protein |
Gene Summary | This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013] |
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