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Tmem30a (NM_001004248) Rat Untagged Clone
CAT＃: RN211350
Tmem30a (untagged ORF) - Rat transmembrane protein 30A (Tmem30a), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC94262 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN211350 representing NM_001004248
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGAACTATAGCGCGAAGGATGAGGTGGACGGCGGGCCCACGGGTCCTCCGGGGGGCGCCGCCA AGACCCGGAGGCCGGATAACACGGCCTTCAAACAGCAACGGTTGCCTGCCTGGCAGCCCATCCTCACCGC CGGCACGGTGCTGCCCACCTTCTTCATCATCGGCCTCATCTTCATTCCCATCGGCATCGGCATCTTCGTC ACCTCCAACAACATCCGTGAGATCGAGGGCAATGTGTTTATGTATTATGGACTGTCTAATTTCTATCAAA ACCATCGTCGTTATGTGAAATCCCGAGATGATAGCCAGTTAAACGGAGACCCTAGTGCTTTGCTTAATCC AAGTAAGGAATGTGAACCCTATCGAAGAAATGAAGACAAACCGATTGCTCCATGTGGGGCCATTGCCAAC AGCATGTTTAACGATACGTTAGAGTTGTTTCTGGTTGCCAATGAATCTGATCCCAAGCCTGTGCCAATTC TTTTGAAGAAAAAAGGTATTGCTTGGTGGACAGATAAAAATGTGAAATTCAGAAATCCACCTGGAAAAGA CAGCCTTCAAGAAAAGTTTAAAGATACAACAAAGCCAGTAAACTGGCATAAACCAGTATATGAGCTAGAC CCTGATGATGAAAGTAATAATGGATTCATAAATGAAGACTTTATTGTTTGGATGCGTACTGCAGCTTTAC CTACTTTTCGTAAGTTATATCGTCTTATAGAGCGGACAGATGATTTACACCCAACACTACCAGCTGGACA GTACTATTTGAACATCACATACAATTACCCTGTACATTTTTTTGATGGACGAAAACGGATGATCTTGAGC ACTATTTCATGGATGGGAGGAAAGAATCCATTTTTGGGAATTGCTTACATCACTATTGGATCCATCTCCT TCCTTCTGGGAGTTGTACTGCTAGTAATTAATCATAAATATAGAAACAGTAGTAATACTGCTGACATCAC CATTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001004248 |
| Insert Size | 987 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001004248.1, NP_001004248.1 |
| RefSeq Size | 1778 bp |
| RefSeq ORF | 987 bp |
| Locus ID | 300857 |
| UniProt ID | Q6AY41 |
| Gene Summary | Accessory component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. The beta subunit may assist in binding of the phospholipid substrate. Required for the proper folding, assembly and ER to Golgi exit of the ATP8A2:TMEM30A flippase complex. ATP8A2:TMEM30A may be involved in regulation of neurite outgrowth, and, reconstituted to liposomes, predomiminantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE). The ATP8A1:TMEM30A flippase complex seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Required for the formation of the ATP8A2, ATP8B1 and ATP8B2 P-type ATPAse intermediate phosphoenzymes. Involved in uptake of platelet-activating factor (PAF). Can also mediate the export of alpha subunits ATP8A1, ATP8B1, ATP8B2, ATP8B4, ATP10A, ATP10B, ATP10D, ATP11A, ATP11B and ATP11C from ER to other membrane localizations (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR211350 | Tmem30a (Myc-DDK-tagged ORF) - Rat transmembrane protein 30A (Tmem30a), (10 ug) |
CNY 3,600.00 |
|
| RR211350L3 | Lenti ORF clone of Tmem30a (Myc-DDK-tagged ORF) - Rat transmembrane protein 30A (Tmem30a), (10 ug) |
CNY 6,080.00 |
|
| RR211350L4 | Lenti ORF clone of Tmem30a (mGFP-tagged ORF) - Rat transmembrane protein 30A (Tmem30a), (10 ug) |
CNY 6,650.00 |
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