B3gnt5 (NM_053932) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216518 representing NM_053932 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGTATTCGTCAGCAGCAGAAGAGTCAAAAGATGGCAGTTTTTTCACTTATTTGCCATTTGTTTTA TATTAAGCTTTATGGTTTTTTGGGGCCCGATCAATAATTACATCATGAGCCACATGAAGTCCTACTCCTA CAGATACCTTATAAATAGCTATGACTTTGTGAACGATTCCCTGTCTCTCAAGCACAGCTCTGTGCAGCCT CACCACCCATACTTGATCAACCACAGAGAGAAGTGTCAGGCCCAAGATGTCCTCCTCTTACTCTTTATAA AGACTGCCCCTGAAAACTACGAGCGACGTTCTGCCATCAGAAAGACGTGGGGCAACGAGAATTACGTCCA GTCTCAGCTCAACGCCAACATCAAAATTCTGTTCGCGTTAGGAACTCCTCATCCACTGAAGGGAAAAGAG CTGCAAAAAAGACTGATTTGGGAAGATCAAGTGTACCACGACATAATTCAGCAAGATTTCACTGATTCTT TCCACAATCTTACTTTTAAATTTCTTCTTCAGTTCGGCTGGGCAAACACCTTTTGCCCACATGCCAGATT CCTGATGACTGCTGATGATGACATATTTATCCACATGCCAAATCTCATTGAATACCTTCAAGGGCTGGAG CAGGTTGGAGTTCGAGACTTTTGGATTGGTCACGTTCACCGAGGGGGCCCTCCTGTTAGAGACAAAAGTA GCAAGTACTATGTTCCCTATGAAATGTACAAGTGGCCAGCCTACCCTGACTATACCGCCGGTGCTGCCTA TGTCGTCTCCAACGATGTAGCTGCCAAAATCTATGAGGCATCACAGACGCTGAATTCCAGCATGTACATA GACGATGTGTTCATGGGCCTCTGCGCCAATAAAGTGGGGGTCGTGCCACAGGACCATGTATTTTTCTCTG GGGAAGGGAAGATTCCTTACCATCCCTGCATCTATGAAAAGATGATAACGTCTCATGGACACTCACAAGA CCTACAGGACCTCTGGGTGGAGGCCACAGATCCTAAAGTGAAGGACATTTCGAAAGGGTTTTTTGGTCAG ATCTACTGCAGGTTAATTAAGATAGTTCTTCTCTGCAGACTGACTTACAGGAACTCATACCCTTGTAGGG CTGCATTTGCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053932
Insert Size	1134 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053932.1, NP_446384.1
RefSeq Size	4209 bp
RefSeq ORF	1134 bp
Locus ID	116740
UniProt ID	Q99NB2
Gene Summary	Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development (By similarity).[UniProtKB/Swiss-Prot Function]

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