Rai1 (NM_001271207) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217706 representing NM_001271207 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCTTTTCGAGAAAGGTGTGGTTTCCATGGCAAACAGCAAAACTACCCACAGACCTCACAGGAGA CATCGCGTCTGGAGAACTACAGGCAGCCGGGTCAGGCTGGGCTAAGCTGTGATCGACAGCGGCTGCTGGC CAAGGACTATTACAACCCTCAGCCCTATACAGGCTATGAGGGCGGCACTGGTACACCTGCAGGCACAGTG GCCACAGCAGCTGCAGACAAGTACCACCGAGGCAACAAGTCCCTGCAGGGGAGGCCAGCTTTCCCCAGCT ATGTTCAAGACAGCAGCCCCTACCCAGGGCGCTACTCTGGTGAGGAGGGGCTTCAGACCTGGGGCAGCCC ACAGCCACCACCTCCGCAGCCACAGCCTCTGCCAGGGGCAGTGAGCAAGTACGAGGAGAACTTGATGAAG AAGACAGTGGTGCCTCCAAACAGGCAGTACCCTGAGCAGGGCCCCCAACTTCCCTTCCGGACTCACGGCC TGCATGTCCCACCACCGCAGCCTCAGCAGCCCCTGGCTTACCCCAAACTCCAAAGGCAGAAACCACAGAA TGACCTTGCCTCCCCTCTGCCCTTCCCCCAGGGCAGCCACTTTCCCCAGCATTCCCAGTCCTTCCCTACC TCCTCCACTTATGCCCCAACAGTGCAGGGTGGTGGGCAGGGGGCCCACTCATACAAGAGCTGCACAGCAC CATCTGCCCAGCCTCATGACAGGCCGATGAGTGCCAATGCGAGCCTGGCTCCAGGGCAACGGGTCCAGAA TCTTCACGCTTACCAGCCGGGCCGCCTTGGCTATGAGCAGCAGCAGCAAGCACTTCAAGGCCGGCACCAC ACCCAGGAAACACTCCACTACCAGAACCTCGCCAAGTACCAACACTATGGACAGCAAGGCCAGGGCTACT GTCCAGCGGACACAGCTGTCAGGACTCCGGAACAGTATTACCAGACCTTCAGCCCTAGCTCCAGCCACTC CCCTGCACGCTCCGTGGGTCGCTCCCCTTCCTACAGCTCCACCCCGTCACCACTGATGCCCAATCTGGAG AACTTCCCTTATAGCCAGCAGCCGCTTAGTACTGGGGCCTTCCCCACAGGCATCACAGACCACAGCCACT TTATGCCCCTGCTCAACCCGTCCCCAACAGATGCTGCCAGCTCTGTGGACCCCCAGGTCAGCAACTGCAA GCCCCTGCAAAAGGAGAAGCTTCCTGACAACTTGCTGTCAGATCTCAGCCTGCAGAGTCTCACAGCGCTC ACCTCACAGGTAGAAAACATCTCCAATACTGTGCAACAGCTCTTGCTGTCCAAAGCTACCATACCACAGA AGAAAGGGGTCAAGAACCTTGTGTCTAGGACTCCAGAGCAGCACAAAAGCCAGCATTGTAGCCCAGAGGG CAGTGGCTACTCAGCTGAGCCAGCAGGCACACCACTGTCTGAGCCACCAAGTAGCACACCACAGTCCACT CATGCTGAACCACAAGACACTGACTACCTGAGTGGCTCTGAGGACCCACTAGAGCGCAGCTTCCTCTACT GCAGCCAGGCCCGGGGCAGTCCTGCCAGAGTCAACAGCAACTCTAAGGCCAAGCCTGAGTCTGTGTCCAC CTGTTCTGTGACCTCACCTGATGACATGTCCACCAAGTCTGACGACTCTTTCCAGAGCCTGCACAGTACT CTGCCACTGGATAGCTTCTCCAAATTTGTGGCAGGCGAGCGGGACTGTCCACGGCTGCTGCTCAGTGCCC TGGCACAGGAAGATCTGGCCTCTGAGATCCTGGGACTGCAGGAAGCCATTGTTGAGAAGGCTGACAAGGC CTGGGCTGAGGCTTCCAGCCTGCCCAAGGACAATGGCAAGCCACCCTTCTCACTGGAGAACCACAGCACT TGCCTGGACACTGTGGCTAAGACATCATGGTCACAGCCGGGGGAGCCAGAAGCCCTACCTGAGCCCCTGC AGCTGGACAAGGGGGGCAGCACCAAGGACTTCAGCCCGGGGCTATTTGAAGACCCCTCTGTGGCCTTTGC CACCACTGACCCGAAGAAAACAACCAGTCCCCTGTCCTTTGGCACCAAACCTTTGCTTGGGACTGCCACT CCTGACCCCACCACGGCAGCATTTGACTGCTTTCCAGATACAACCACTGCCAGCTCAGTGGATGGTGCCA ACCCCTTTGCCTGGCCAGAGGAGAACCTGGGCGATGCTTGTCCTCGCTGGGGCCTCCACCCTGGTGAGCT TACCAAGGGCTTGGAGCAGGGTGCAAAAGCCTCAGACAGTGTGGGCAAGGCAGATGCACACGAGACCTCT GCCTGCTTGGGCTTCCAGGAAGAGCATGCCATCGGGAAGCCAGCAGCTGCACTGTCTGGGGACTTCAAGC AGCAGGAGGCAGATGGGGTAAAAGAGGAAGTGGGTGGGTTGCTGCAGTGCCCCGAGGTGGGCAAGGCCGA CCAGTGGCTGGAGGATAGCCGGCATTGCTGTTCCTCCGCTGACTTTGGGGACCTGCCACTGTTGCCACCC CCTGGCAGGAAGGAGGATCTGGAAGCTGAAGAGGAATACTCTTCGCTATGTGAACTGCTGGGGAGCCCTG AGCAGAGGCCCAGCCTGCAGGACCCACTGTCCCCCAAGGCCCCTCTGATTTGTACCAAGGAGGAAGCAGA GGAGGTACTAGATACCAAGGCTGGCTGGGCCTCTCCGTGCCACCTCTCTGGGGAGCCCGCAGTCCTTCTG GGCCCCTCCGTGGGTGCCGAATCAAAGGTCCAAAGCTGGTTTGAATCTTCCCTGTCACATATGAAGCCAG GAGAAGGGCCAGAGCTGGAGAGAGCTCCAGGTAGCGCCAGCACTTCACAGGGCTCCTTGGCCCCAAAGCC TAACACGCCCGCCGTACCCGAGGGACCCATTGCGAAGAAAGAGCCTGTGCCACGGGGTAAAAGTTTACGG AGCCGGCGAGTACACCGGGGGCTGCCTGAGGCGGAAGACTCTCCATGCAGGGCGCCAGCACTTCCCAAAG ACCTCTTGCTCCCAGAGTCCTGCACGGGGCCCCCACAGGGTCAGGCAGAGGGGGCCGGGGCCCCAGGCCG GGGACTGTCAGAAGGGCTCCCCAGAATGTGTACCCGTTCTCTCACAGCTCTGAGTGAGCCCCAAACTCCT GGACCTCCAGGCCTGACTACCACCCCCACACCTCCTGACAAACTGGGAGGAAAACAGCGAGCTGCCTTCA AGTCTGGTAAGCGGGTAGGAAAACCGTCACCCAAGGCTGCATCCAGCCCCAGCAACCCTGCTGCCCTGCC TGTTGCCTCAGACAGCAGCCCCATGGGCTCCAAAACCAAGGAGCCAGACTCTCCCGGCATGCCTGGCAAG GACCAGCGGTCCATGGTCCTCCGGTCTCGCACCAAACCCCAGCAGGTCTTCCATGCCAAACGGCGGCGGC CCTCAGAGAGCAGGATCCCAGACTGCCGTCCCACCAAGAAGCCCCCTGCTAACAACCATTTACCCACTGC ATTCAAGGTCTCCAGTGGGCCCCAGAAGGAAGGTCGGATGAGCCAGCGGGGCAAAGTACCCAAGCCCGGT ACAGGGAGCAAGCTTTCAGATAGGCCTCTCCACACACTCAAAAGGAAGTCAGCTTTCATGGCACCTGTCC CTGCCAAAAAGCGGAGCCTCATCCTGCGAAGCAACAATGGAAGTGGGGTAGATGGGAGGGAGGAAAGGGC TGAGAGCTCCCCTGGCCTCTTAAGGAGGATGGCCTCACCCCAGAGGGCCAGACCCAGGGGCAGTGGGGAG CCACCCCCACCCCCACCCCTAGAGCCACCTGCTGCATGCCTGGGGTTGGCTACACAATCATCCCTGCCCA GTGCTGTGAGGACCAAGGTGCTGCCACCCCGAAAGGGCCGTGGCCTCAAGCTGGAGGCCATAGTACAGAA GATCACCTCACCTGGTCTCAAGAAACTTGCATGCAGAGTGGCAGGGGCCCCTCCTGGGACACCCCGGAGC CCTGCCCTACCTGAGAAACGTTCAGGGGGCAGTCCAGCTGGGGCAGAAGAGGGTGTAGGAGGAATGGGTG CAGGGCAAATGCTACCAGCAGCTTCGGGAGCTGACCCATTGTGCAGAAACCCAGCCAGCAGCAGGTCCCT AAAAGGTAAACTCTTGAATAGTAAGAAGCTGTCCTCTGCTGCTGACTGCCCCAAAGCTGAGGCCTTCATG TCCCCGGAGACCCTCCCATCCCTAGGGACTGCCCGGGCACCGAAGAAAAGGAGCCGGAAAGGCAGGACTG GGGCCTTGGGACCCTCCAAAGGTCCATTGGAGAAGCGGCCCTGTCCTGGCCAAGCTCTGATCCTCGCTCC CCACGACAGGGCCAGCAGCACTCAGGGCGGAGGTGAGGACAACTCCAGTGGAGGAGGCAAGAAGCCAAAG ACAGAGGAGCTGGGACTGGCCTCCCAGCCCCCAGAAGGCCGGCCCTGCCAGCCCCAGACAAGGGCACAGA AGCAGCCAGGCCAAGCCAGCTATAGCAGCTATTCAAAGCGGAAGCGCCTCAGCCGTGGCCGAGGAAAGGC CACCCATGCTTCACCCTGTAAAGGCCGTGCCACTCGGAGAAGGCAGCAGCAGGTACTGCCCCTGGATCCT GCAGAGCCTGAAATCCGACTCAAATACATTTCTTCTTGCAAGAGGCTGAGGGCAGACAGCCGCACCCCAG CCTTCTCGCCCTTTGTGCGGGTGGAGAAGCGAGATGCATACACCACCATATGCACTGTTGTCAACTCCCC AGGGGATGAGCCGAAGCCTCACTGGAAGCCATCCTCTGTTGCCTCCTCTTCCACCTCCTCCTCAGAACCA GCTGGGGCTTCTCTGACCACATTCCCTGGAGGCTCTGTGCTACAGCTGAGGCCCTCCCTGCCCCTCTCCT CCACCATGCATCTGGGGCCGGTGGTGTCCAAGGCCCTAAGTACCTCTTGCCTTGTCTGCTGCCTCTGCCA AAACCCGGCCAATTTCAAGGACCTTGGGGACCTCTGTGGTCCCTACTACCCTGAACACTGCCTCCCAAAA AAGAAGCCAAAACTCAAGGAGAAGGTACGGCTGGAGGGCACCTTGGAGGAGGCCTCTCTGCCTCTGGAGA GAACACTCAAAGGCCTGGAATGTGCAGCCAGCACCACGGCTGCCACCCCTACCACAACCACCATCACTAC CACCACAACCCTGGGGAGGCTGTCCAGGCCTGATGGCCCAGCCGACCCTGCCAAGCAGGGTTCCCTGCGC ACCAGTGCCCGGGGCCTGTCAAGGCGGCTGCAGAGTTGCTATTGCTGTGATGGTCAGGGGGACGGGGGTG AGGAGGTGGCCCCAGCTGACAAGAGCCGCAAACATGAATGCAGCAAAGAGGCCCCTGCAGAACCTGGTGG GGACACCCAAGAGCACTGGGTACATGAGGCCTGTGCTGTGTGGACCAGCGGGGTGTACCTGGTGGCCGGG AAGCTCTTTGGGCTGCAGGAGGCCATGAAAGTAGCTGTGGACATGCCATGTACCAGCTGTCATGAGTCCG GAGCAACCATCTCGTGCTCCTATGAAGGCTGCACCCACACCTACCACTACCCATGTGCCAATGACACAGG TTGCACGTTCATTGAGGAGAATTTTACTTTGAAGTGCCCCAAACATAAGAGGCTGCCGTTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271207
Insert Size	5664 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271207.1, NP_001258136.1
RefSeq Size	7600 bp
RefSeq ORF	5664 bp
Locus ID	303188

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