Svil (NM_001108416) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217749 representing NM_001108416 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAGAAAAGAAAGAATTGCTAGGCGCTTAGAAGGAATAGAAAATGACCCCCAGCCTATCCTGCTGC AGAGCTGCACAGGGCTAGTGACTCACCGGCTGCTGGAAGAGGACACGCCCAGATACATGCGCGCCACAGA TCCTGCAAGCCCTCATACTGGGCGATCCAATGAAGAGGAAGACACTCCTGGGTCCTCCCTTGAAAAGCAA ACTCCTAAATACGGCACAGAAACCTCGGGCATCCCTAGTTCGGGCTCCATGGACACCCACAGTCTGGAGT CCAAAGCGGAAAGGATCGCAAGATACAAAGCAGAGAGAAGGCGGCAGCTGGCGGAGAAGTACGGGCTGAC CCTGGACCCTGAAACCGACTCCGAATACTTGTCTCGATATGCCAAATCTCGGAAGGACCCGGATGTAGCT GAGAGAAGAGGGAAAAGTGACAAGCAAGAAGAACAGAGCAAAGATGCCAGTTCCCGGCACTCCAGGACGG AGTCAGGGCCCAGGACCAGCTTGGTGGTGTCCCAGGACGGTGCCCCGCTAGGAAGCAACTTGTCTGACCA GGAGCTGCTGCTCAATGTGGAGAACCAGCGAAGAGTCCAAGAGCCCACACTAGGAGAAGATGGTTCCTCT GCCTTCTTTTCTGAGCGCAGCACCTCCTTTTCCGAAGTGCCCAGGTCTCCAAAGCACATACCCAGCTCAC CGCTGCAACAGCCAGCCTCCCCGAGCCTCCCCGGTGACTCCCCACTGTCTACAGAGGCGCGAGCCAGCAC AGGGAGGCCCACACACGAGTGGTTTCTGCAGAGAGACTCTGAAGGCGACACGCCCTCACTTATCAACTGG CCTTCCAGAGTTAAAGTTAGAGAAAAATTGGTGAAAGAGGAGAGTGCTCGAAGCAGCCCTGAACTTACCT CAGAGTCCCTAACTCAGAGGAGACAGCAGCCAGCACCAGCTCATTTCCTGTCTATCCAGTCAGAAAGTTC CGCCTTTGATAGGGTCACCAGCAAAGCAGTGAGCTCATTGCGGCCAAGCCGGAGCGGTGTCCTGCCAGCT GACCCTGTTCATGCCATCAAGGTGGTGACCATGGACACCCCAGAAAGCTCATCTGAATGCAGCTGGGTGG GGTCAGCCACCCCAAAAGTTATAAAATCTACCACCCTGAAGATTCTAGAAGGTGGGTCAAGGGACGCACC AGTACTCCATATTCATGAGTCAAAAGCAGAAGATGAGCTCTCCCCTGAACCTCTGGAGAGGAACCGCAAA TCACTTTTGACCTCAGAAGATGACGGGCTTGTGAGAGGCCACAAAGACCCCTCTGATGATAAAGATTTAG AGAAGGCCACCATTCGCTCCATCAACGTAGAACCTGAGAGGGAGCAGCAAGTTCAGAGAACTGGCCGGAG TGAGATGGTTCTGTATGTTCAGAGTGCTCCTGTGTCCCAAGATGCCACATCGACCAGTCCCTCAAAGGAG GCATCACCAAAGAAGCGCAAGGTTGTGGCCCGTTCTCTCTCGGATTACACGGGTCCCCCGCAGCTCCAGG CCCCCAGACACAAGGATGCAACCCCAAAGCAGGCACTGGAGCTGCAGAGTCCCAGGGTAGAAGGGCCTGG TGCAGAAGCCAGTATTCTGGACACCAGAGTTTCTGTTGCCCAGCTCCGGAATGTATTTATGGAGTCGACC AGAGCCAGCAAGAAACCCGAACTCCAATCCCGGGTTGAGAGGTCAGCTGAAGGAATTGGCTTGCCCATGG AACGGGAGAGAGGGTCTCGGAAACCAAGACGCTATCTTTCTCCTGGTGAAAGTAGAAAAACTTCTGAGCG ATTTAGAACTCAACCTATAACTTCAGCAGAACGAAAGGAGTCGGATAGGTACCCTTCTGGTTCAGAATTG CCAGTGGTCGAGGATGAAGAAAAGGTGGATGAACGGGCCAAGCTGAGCGTTGCTGCCAAGAGGCTGCTTT TCAGGGAAATGGAGAAATGCTTTGATGAGCACAGCATCCCAAAAAGGCGTTCAAGAAATGCAGCCGTGGA GCAGAGGCTACGTCGCCTGCAGGACCGTTCACACACACAGCCCATCACCACGGAGGAAGTGGTCATTGCA GCCACTGAACCTATCCCTGCTTCGTGTTCTGGGGTCACCCACCCCGTAATGGCGAGACTTCCTAGCCCCA CTGTAGCTAGAAGCACTGTGCAGCCTTCCAGGTTACAGGCATCTGCTCACCAGAAGGCTTTAGCCAGGGA CCTGGCAAATGAGGGCAGAGAGTCTGCTGAACCAGGCGAACCTGATTCCTCCACTCTGAGCTTAGCAGAG AAATTGGCCTTGTTTAACAAATTGTCCCAGCCAGTCTCCAAAGCCATTTCCACCCGAAACAGAATAGACG TGCGGCAGAGGAGGATGAACGCAAGGTATCAGACCCAGCCGGTCACACTTGGAGAGGTGGAGCAGGTGCA GAGTGGGAAGCTCATCTCCTTCTCCCCTACTGTGAACACCTCTGTTTCTACCATGGCTTCTGCAGTTGCT CCCACATATGCAGGGGACCTTCGAAAGCCGTCTGTTGACAACAGTGCAAGTGCCCCTGACTATAAGTCTC CTTCTCCTGTGGAAAACTCAGACTCTCCAGTCAGAAGCATTCTGAAACCACAAGCCTGGCGGCCTTTGGC AGAGCACAGTGGGAGCAAAGGAATGCCGGAGGAGTCCGGAGAGACGGAAAGCAAGAATTCTCTGACTGCA GCTGCTGAAGACAGTCACATTCAAACACGAGGAGCCGTGGAGGAGGAAGTGGAGCCCTCCTACCCCATCC TTGGCAGAGTCGGGGAGGGTGATGGTCAGAAGGAGCCTAAGTATGCCAACCTCAGGAGGGGAAGCTTAGA GCTAGGGAATTCTTCTGCCACACACCTTGGTGATGAGCTGAAGGAATTTTCCACTGCTAAAAGCAGTTTG CAAGAGAGTCTGGACCTGAAGGATAAGCAGGCCTCGGAGGAGAATGCAGATGTGGAGAATGTCATGAGGA AGTTCTCACTCAAAGAGTTTGGAGAAACTGCTTCTGAGAAGACGGAGACAGCTGCCAGGAAAGCCTCTGT TCCGATGGCAACCCCAGGGGCCTGGAAGCAGCAGGAGTCCTCTGAGCAGCTAGCAGAGAAGCTCTTCAAG AACCCCTGTGCCATGTTTGCTTCTGGAGAGGTCAAAGTGCCAGCGGGCGACAGTAGCCTGGATTCACCCA GCAAAACCATGTCAATCAAAGAAAGGCTAGCTCTGTTGAAGAAGAGTGGCGAGGAAGACTGGAAGAACAG GCTCATTCGGAAGCAGGAATACAGCAAGGCCACTGGTGGCCTGCACTCACCGGAGGTGGAGCAGTCCCTG AAGAAAAAGAGAGTCACAGAAAGTCGAGAGAGCCAGATGACGATTGAAGAGAGGAAGCACCTCATCACTG TGCGCGAGGAAGCTTGGAAGACAAAGGGCAGAGGCGCTGCGAATGACTCCACACAGTTCACCGTGGCAGG AAGAATGGTGAAGAAAGGTCTGGCATCACCCACGGCCATAACTCCCATCTCATCCCCTCTCTGCAGTAAA TCAAGGGGCACGACCCCAGTTTCCAAACCCTTGGAAGATATTGAAGCCAGACCAGATATGCAGCTGGAAT CGGACCTGAAGTTGGACAGGCTGGAAACCTTCCTAAGGAGGCTGAATAACAAAGTTGCTGGGATGCAGGA AACAGTTCTCACAGTCACTGGCAAATCTGTCAAGGAGGTGATGAAGTTGGATGATGATGAAACCTTTGCC AAATTCTACCGCAGTGTGGATCACAGTTTACCCAGAAGTCCTGTGGAGCTGGAGGAGGACTTCGATGTCA TTTTCGACCCTTATGCTCCCAAGTTGACATCTTTGGTGGCAGAGCATAAGCGCTCAGTCAGGCCCAAGCG CCGGGTCCAGGCTTCCAAGAACCCTCTCAAACTGTTGGCAGCAAGAGACGACCTCCTTCAGGAATACACG GAGCAAAGGCTAAATGTTGCCTTCATGGAGTCCAAGCGGATGAAAGTGGAGAAGATGTCCTCCAACTCCA ACTTCTCAGAAGTGACGCTGGCGGGCCTAGCCAGTAGGGAGAACTTCAGCAGCATCAGCCTGCGGAGTGT CAACCTGATGGAGCAAAACTCCAACAACAGTGCCCTGCCCTACAAGAAGCTCATGCTGTTGCAGATTAAA GGAAGAAGACACGTGCAGACAAGGCTGGTGGAGCCTCGAGCCTCATCACTCAACAGTGGGGACTGCTTCC TTCTACTCTCTCCTCAGTACTGCTTCCTGTGGGTGGGAGAGTTCTCCAATGTCATTGAGAAAGCAAAGGC ATCTGAACTTGCGACATTAATTCAGACAAAGAGGGAGCTTGGTTGTAGAGCTACATACGTCCAGACCATT GAAGAAGGAATTAACACACATACTCACGCAGCCAAAGACTTCTGGAAGCTTCTGGGTGGTCAGACCAGTT ACCAGTCTGCTGGAGACCCAAAGGAAGATGAGCTGTACGAGACGGCCATCATAGAGACAAACTGTGTCTA CCGCCTGACAGACGACAAACTCGTTCCTGATGATGACTACTGGGGGAAGATTCCCAAGTGTTCCCTTCTG CAGTCCAAAGAGGTGCTGGTGTTTGACTTTGGTAGTGAAGTTTACGTGTGGCATGGGAAGGAAGTCACGT TAGCACAACGGAAAATAGCATTCCAGCTGGCCAAGCACTTATGGAATGGAACTTTTGACTATGAGAACTG TGACATCAACCCCCTGGACCCTGGAGAGTGCAACCCGCTCATTCCCAGGAAAGGACAAGGGCGGCCTGAC TGGGCAATATTTGGAAGAGTTACGGAACACAATGAGACTATTCTGTTCAAAGAGAAATTCCTCGACTGGA CAGAACTGAAGAGACCCACAGAGAAGAATTCTGGGGAAGTTGTCCACCAGAAGGATTACCCGAGGGCTGA CGTCAAGCCCTATGATGTGACAAGGATGGTGGCAACGCCCCAGATGACAGCCAGCACCATCTTGGATGGG GTGAATGTTGGCCGTGGCTATGGCCTGGTAGAAGGAGATGACAGAAGACAGGTTGAGATTACCACCGTCT CTGTGGATGTGTGGCATATTCTGGAATTTGACTACAGCAGGCTCCCCAGGCAGAGCATAGGACAGTTCCA CGAGGGAGACGCCTACGTGGTCAAGTGGAAGTACATGGCGAGCACAGCAGTGGGAAGCCGACAAAAGGGG GAACATCCGGTGAGAGTGGCTGGCAAAGAGAAGTGTGTCTATTTCTTCTGGCAAGGCCGGCACTCGACCG TGAGTGAGAAGGGAACGTCAGCTCTCATGACAGTGGAACTGGATGAAGAGAGGGGGGCCCAGGTCCAGGT CCTGCAGGGAAAGGAGCCACCCTGCTTCCTTCAGTGTTTCCAGGGGGGTATGGTGGTACACTCCGGACGG AGGGAAGAGGAAGAAGAAAATGTGCAAAGTGAATGGAGACTGTATTGTGTTCGAGGAGAGGTGCCCATGG AAGGGAACTTGCTAGAAGTGGCCTGTCACTGCAGCAGCCTGAGGTCCAGGACATCCATGGTTGTTCTGAA CATAAACAAGGCCCTCATTTACCTGTGGCACGGATGCAAAGCCCAAGGCCATACGAAGGAGGTTGGAAGG ACTGCGGCAAATAAAATCAAGGAAGAATGTCCCCTGGAAGCAGGCCTGCACAGCAGCAGCAACGTGACAA TCCATGAGTGTGACGAAGGATCCGAGCCCCTGGGATTCTGGGACGCTCTGGGAAGGAGGGACAGGAAGGC CTATGACTGCATGCTTCAAGATCCTGGAAGTTTTAACTTCGCGCCCCGCCTGTTCATCCTCAGCAGCTCC TCAGGAGACTTCTCAGCGACAGAGTTCGTGTACCCCGCGCGAGCGCCCTCTGCCGTCAGCTCCATGCCTT TCCTGCAGGAGGATCTGTACAGCGCGCCGCAGCCAGCTCTCTTCCTTGTTGACAACCATCACGAGGTGTA CCTCTGGCAAGGCTGGTGGCCCACCGAAAACAAGATAACCGGCTCTGCTCGCATTCGCTGGGCCTCAGAC CGGAAGAGCGCCATGGAGACAGTCCTGCAGTACTGCCGAGGAAAGAATCTCAAAAGGCCACCCCCCAAGT CTTACCTTATCCATGCTGGGCTAGAGCCCCTGACGTTCACTAACATGTTTCCCAGCTGGGAACACAGAGA AGACATTGCCGAAATCACAGAAATGGACACTGAAGTTTCCAACCAGATCACCCTGGTGGAAGACGTCTTA GCCAAGCTGTGTAAAACCATCTATCCTCTGGCAGATCTACTTGCCAGGCCACTCCCAGAGGGGGTAGACC CTCTAAAGCTTGAGATTTATCTTACAGATGAAGACTTCGAGTTTGCACTCGACATGACCAGAGATGAATT CAACGCACTGCCCACCTGGAAGCAAATGAACCTGAAGAAAGCGAAAGGCCTGTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108416
Insert Size	6498 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108416.2, NP_001101886.2
RefSeq Size	8155 bp
RefSeq ORF	6498 bp
Locus ID	361256

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