Gucy1b3 (NM_012769) Rat Tagged ORF Clone
CAT#: RR202667
- TrueORF®
Gucy1b3 (Myc-DDK-tagged ORF) - Rat guanylate cyclase 1, soluble, beta 3 (Gucy1b3), (10 ug)
"NM_012769" in other vectors (3)
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CNY 7,980.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Gucy1b1; SGC |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202667 representing NM_012769
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGGTTTTGTGAACCATGCCCTGGAGCTGCTGGTGATCCGCAATTACGGTCCCGAGGTGTGGGAAG ACATCAAAAAAGAGGCGCAGCTGGATGAAGAAGGCCAGTTTCTTGTGAGAATAATCTACGATGATTCCAA AACCTATGACTTGGTGGCTGCTGCGAGCAAAGTCCTCAACCTCAATGCTGGTGAAATCCTGCAGATGTTT GGGAAGATGTTTTTCGTCTTCTGTCAAGAGTCTGGCTATGATACCATCTTGCGTGTCCTGGGATCTAATG TCAGGGAGTTTTTGCAGAACCTCGACGCCCTGCACGACCACCTCGCCACCATCTACCCAGGGATGCGCGC ACCTTCCTTCCGGTGCACCGATGCAGAAAAAGGCAAAGGGCTCATTCTGCACTACTACTCGGAAAGAGAG GGGCTTCAGGACATTGTGATCGGGATTATCAAGACTGTAGCTCAACAGATCCATGGCACTGAGATAGACA TGAAGGTTATTCAGCAAAGAAGTGAAGAATGTGATCATACCCAATTTTTAATTGAAGAAAAAGAATCAAA AGAAGAGGATTTTTATGAAGATCTGGACAGGTTTGAAGAGAACGGTACCCAGGACTCCCGTATCAGCCCG TACACCTTCTGCAAAGCGTTTCCTTTTCACATCATATTTGACCGGGACCTAGTAGTCACGCAGTGTGGAA ATGCTATCTACAGAGTGCTCCCCCAGCTCCAGCCTGGGAAGTGCAGCCTTCTGTCTGTCTTCTCTCTGGT CCGCCCTCATATTGACATCAGTTTCCACGGGATTCTTTCACACATCAATACCGTCTTTGTACTGAGAAGC AAGGAAGGGTTGCTGGATGTTGAGAAACTTGAATGTGAGGATGAACTGACTGGGGCAGAGATTAGCTGCC TCCGTCTCAAAGGCCAAATGATCTATTTACCGGAAGCAGATAGCATCCTCTTCCTCTGTTCACCAAGTGT GATGAACTTGGATGACCTAACAAGAAGAGGCCTGTACCTGAGTGACATCCCTCTCCATGATGCTACACGA GACCTGGTCCTTTTGGGAGAACAGTTCCGGGAGGAGTACAAACTGACACAAGAGCTGGAAATCCTCACAG ACAGGCTGCAGCTCACACTGAGGGCTTTGGAGGATGAGAAGAAAAAGACAGACACATTGCTATATTCTGT TCTCCCTCCATCTGTTGCCAATGAGCTGAGACACAAGCGTCCAGTGCCGGCCAAAAGATACGACAATGTG ACCATCCTCTTCAGTGGCATTGTGGGCTTCAACGCTTTCTGTAGCAAGCATGCATCTGGAGAAGGGGCCA TGAAGATTGTCAATCTACTCAACGACCTCTACACCAGATTCGACACACTGACTGATTCACGGAAAAACCC ATTTGTTTACAAGGTGGAAACAGTTGGTGACAAGTATATGACAGTGAGTGGTTTGCCAGAACCTTGTATC CACCATGCACGGTCCATTTGCCACCTGGCTTTAGACATGATGGAAATTGCTGGTCAAGTTCAAGTAGATG GCGAATCTGTTCAGATAACCATCGGGATCCACACTGGGGAGGTGGTGACAGGTGTGATTGGACAGCGGAT GCCACGGTATTGTCTCTTCGGAAATACTGTCAACCTCACAAGCAGGACAGAAACCACAGGAGAAAAGGGG AAGATAAACGTTTCCGAATATACATACAGGTGTCTCATGTCCCCAGAAAACTCGGATCCACAGTTCCACT TGGAGCACAGAGGTCCTGTGTCCATGAAGGGCAAGAAGGAGCCAATGCAAGTCTGGTTTCTATCCAGGAA AAACACAGGCACAGAGGAAACAAACCAGGATGAAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202667 representing NM_012769
Red=Cloning site Green=Tags(s) MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMF GKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSERE GLQDIVIGIIKTVAQQIHGTEIDMKVIQQRSEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQDSRISP YTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGKCSLLSVFSLVRPHIDISFHGILSHINTVFVLRS KEGLLDVEKLECEDELTGAEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATR DLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNV TILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCI HHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKG KINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETNQDEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012769 |
ORF Size | 1857 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012769.2, NP_036901.2 |
RefSeq Size | 3217 bp |
RefSeq ORF | 1860 bp |
Locus ID | 25202 |
UniProt ID | P20595 |
MW | 70.5 kDa |
Gene Summary | subunit of soluble guanylate cyclase, which catalyzes the conversion of GTP to cyclic GMP [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202667 | Gucy1b3 (untagged ORF) - Rat guanylate cyclase 1, soluble, beta 3 (Gucy1b3), (10 ug) |
CNY 7,980.00 |
|
RR202667L3 | Lenti ORF clone of Gucy1b3 (Myc-DDK-tagged ORF) - Rat guanylate cyclase 1, soluble, beta 3 (Gucy1b3), (10 ug) |
CNY 9,790.00 |
|
RR202667L4 | Lenti ORF clone of Gucy1b3 (mGFP-tagged ORF) - Rat guanylate cyclase 1, soluble, beta 3 (Gucy1b3), (10 ug) |
CNY 10,740.00 |
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