Btla (NM_213630) Rat Tagged ORF Clone
CAT#: RR205257
- TrueORF®
Btla (Myc-DDK-tagged ORF) - Rat B and T lymphocyte associated (Btla), (10 ug)
"NM_213630" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR205257 representing NM_213630
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACAGTGCCTGCCATGCTTGTCACTCCCCGGTCCTTTAGGGAATTCTTCATCCTTCTTCTGGGCC TCTGGAGCATCCTTTGTAAGGAACCTACTAAGAGGATCGGTGAAGAATGCAGAGTGCAACTTAAAATTAA GAGGAATTCTAGCCGCTCTGCCTGGACAGGAGAGTTATTTAAAATTGAATGTCCTGTGACATACTGTGTT CACAGACCTAATGTGACTTGGTGTAAGCACAATGGAACAAGATGTGTACCCCTTGAGGTTGGACCCCAGC TGCACACTAGTTGGGTTGAAAATGACCAGGCTTCAGCTTTTGTTCTCTATTTTGAACCAATACATCTCAG TGATGACGGGGTATATACCTGTTCTGCGAACTTGAATTCTGAGGTTATTAATAGCCACTCAGTAGTCATC CATGTGACAGAAAGGACTCAAAACTGTTCAGAACACCCTCTAATAACAGCATCTGACATCCCAGATGCTA CCAATGCCTCACGACCATCTACCATGGAAGAGAGGCCAGGCAGGACCTGGCTGCTTTACGCCTTGCTTCC CTTGGGGACATCGCTTCTGCTCCTTGCCTGTGTCTGCCTGCTCTGCTTTCTGAGAAGGATCCAAGGGAAA GAAAAGAAGCCTTCTGACTTAGCAGGAAGAGAAAGGGAAACTAACTTGGTTGATATTCCAGTGAGTTCCA GGACAAACTCCCAAATACTGACATCAGAAACTGGAATTTATGACAATGACCCCTGGTCCAGTAGGCTGGG AGAATCTGAATCAACCATTAGCTCACAATTGGAGGGAAACAAACAGGGCATTGTTTATGCCTCTCTGAAC CATTGTGTTATTGGAAGGACCCCAAGACAGGCAAGCAAGATTCAGGAGGCACCCACAGAATATGCATCCA TTTGTGTGAGAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205257 representing NM_213630
Red=Cloning site Green=Tags(s) MKTVPAMLVTPRSFREFFILLLGLWSILCKEPTKRIGEECRVQLKIKRNSSRSAWTGELFKIECPVTYCV HRPNVTWCKHNGTRCVPLEVGPQLHTSWVENDQASAFVLYFEPIHLSDDGVYTCSANLNSEVINSHSVVI HVTERTQNCSEHPLITASDIPDATNASRPSTMEERPGRTWLLYALLPLGTSLLLLACVCLLCFLRRIQGK EKKPSDLAGRERETNLVDIPVSSRTNSQILTSETGIYDNDPWSSRLGESESTISSQLEGNKQGIVYASLN HCVIGRTPRQASKIQEAPTEYASICVRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_213630 |
ORF Size | 924 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_213630.1, NP_998795.1 |
RefSeq Size | 927 bp |
RefSeq ORF | 927 bp |
Locus ID | 407756 |
UniProt ID | Q6PNM1 |
MW | 34.5 kDa |
Gene Summary | Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN205257 | Btla (untagged ORF) - Rat B and T lymphocyte associated (Btla), (10 ug) |
CNY 3,600.00 |
|
RR205257L3 | Lenti ORF clone of Btla (Myc-DDK-tagged ORF) - Rat B and T lymphocyte associated (Btla), (10 ug) |
CNY 6,080.00 |
|
RR205257L4 | Lenti ORF clone of Btla (mGFP-tagged ORF) - Rat B and T lymphocyte associated (Btla), (10 ug) |
CNY 6,650.00 |