Bbs2 (NM_053618) Rat Tagged Lenti ORF Clone
CAT#: RR205555L4
- LentiORF®
Lenti ORF clone of Bbs2 (mGFP-tagged ORF) - Rat Bardet-Biedl syndrome 2 homolog (human) (Bbs2), (10 ug)
"NM_053618" in other vectors (3)
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CNY 12,160.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | mGFP |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR205555).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053618 |
ORF Size | 2163 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053618.1, NP_446070.1 |
RefSeq Size | 2573 bp |
RefSeq ORF | 2166 bp |
Locus ID | 113948 |
UniProt ID | Q99MH9 |
Gene Summary | The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN205555 | Bbs2 (untagged ORF) - Rat Bardet-Biedl syndrome 2 homolog (human) (Bbs2), (10 ug) |
CNY 9,220.00 |
|
RR205555 | Bbs2 (Myc-DDK-tagged ORF) - Rat Bardet-Biedl syndrome 2 homolog (human) (Bbs2), (10 ug) |
CNY 9,220.00 |
|
RR205555L3 | Lenti ORF clone of Bbs2 (Myc-DDK-tagged ORF) - Rat Bardet-Biedl syndrome 2 homolog (human) (Bbs2), (10 ug) |
CNY 11,020.00 |