Uba1 (NM_001014080) Rat Tagged Lenti ORF Clone
CAT#: RR211933L4
- LentiORF®
Lenti ORF clone of Uba1 (mGFP-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)
"NM_001014080" in other vectors (3)
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CNY 20,330.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | mGFP |
Synonyms | Ube1x |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR211933).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001014080 |
ORF Size | 3174 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001014080.1, NP_001014102.1 |
RefSeq Size | 3618 bp |
RefSeq ORF | 3177 bp |
Locus ID | 314432 |
UniProt ID | Q5U300 |
Gene Summary | Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN211933 | Uba1 (untagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug) |
CNY 17,010.00 |
|
RR211933 | Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug) |
CNY 17,010.00 |
|
RR211933L3 | Lenti ORF clone of Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug) |
CNY 18,620.00 |