Sbf1 (NM_001271173) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217707 representing NM_001271173 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGCTCGCGGACTACTTCGTGCTGGTGGCGTTCGGGCCGCACCCGCGCGGGAGTGGGGAAGGCC AGGGCCAGATCCTGCAGCGCTTCCCGGAGAAGGACTGGGAGGACAACCCTTTCCCCCAGGGCATTGAGCT GTTTTGCCAGCCCAGTGGGTGGCAGCTATGTCCCGAGAGGAATCCACCAACTTTCTTTGTGGCTGTCCTT ACTGACATCAACTCTGAGAGGCACTACTGCGCCTGCTTGACCTTCTGGGAGCCAGTGGAGTCCACACAGG AAGTGATGTGCACTGAAGATGCCACAGAGAAGGAGGAAGAGGCACATGGAGGAGGCCAAGCACAGCTGTC ATCCACAGCCCCAGCCCAACCTGGCCAGCTTTTTGCTCCAAAGACTCTGGTTCTGGTGTCTCGACTGGAC CATGCTGACGTGTTCAGGAATAGCCTTGGTCTCATCTATACCATCCATGTGGAAGGCCTGAACGTGAACC TGGAGAATGTGATTGGGAATCTGCTCACGTGCACCGTCCCCTTGGCAGGCGGGTCTCAGAGAACCATCTC TTTGGGGGCTGGTGACCGGCAGGTCATTCAGACCCCACTGGTAGACTCACTGCCTGTCAGCCGCTGTAGT GTAGCCCTGCTCTTCCGCCAGCTGGGCATCACCAATGTGCTGTCTTTGTTCTGTGCTGCCCTCACTGAGC ACAAAGTCCTCTTCTTGTCCAGGAGCTACCAACGTCTAGCCGATGCTTGCAGGGGCCTCTTGGCACTGCT GTTTCCTCTCAGATACAGCTTCACATATGTGCCCATCCTGCCGGCACAGCTGCTCGAAGTCCTTAGTACA CCTACACCTTTCATCATTGGGGTCAATGCAGCCTTCCAGGCAGAGACTCAGGAGCTGCTGGACGTGATTG TTGCCGATCTTGATGGAGGGACAGTGACTGTCCCTGAGTGTGTGCACATTCCACCCCTGCCAGAGCCACT ACAAAGCCAGACTCACAATGTTCTGAGCATGGTCCTGGATCCAGAGCTGGAGTTGGCTGACCTTGCCTTT CCACCACCTACAACATCTGCTTCCTCACTGAAAATGCAGGACAAGGAGCTCCGCGCTGTCTTTCTGCGGC TCTTTGCTCAGCTCCTGCAGGGTTACCGCTGGTGCCTGCACATCGTGCGCATCCACCCCGAGCCTGTCAT TCGCTTCCATAAGGCAGCGTTCTTAGGCCAGCGTGGACTGGTGGAGGATGATTTCCTGATGAAAGTGTTG GAGGGCATGGCCTTTGCAGGCTTTGTGTCAGAGCGTGGGGTCCCCTACCGTTCTACAGACCTGTTTGATG AGTTGGTGGCTCATGAGGTAGCACGGATGCGAGCAGATGAGAACCATCCCCACCGCGTCCTGCGTCACGT CCAAGAACTAGCAGAGCAACTTTATAAGAATGAAAACCCGTACCCAGCTGTGGCAATGCATAAAGTGCAG AGGCCAGGAGAGGCCAGCCACCTGCGGCGGACCCATCGGCCATTCCCCCGGCTAGATGAGGGCACAGTTC AGTGGATTGTGGACCAGGCTGCAGCCAAGATGCAGGGTGCACCTCCAGCTGTGAAGGCTGAAAGGAGGAC CACTGTCCCTTCAGGGCCCCCCATGACGGCCATCCTAGAGCGGTGCAGTGGGCCTCACATCAACAGTGCC CGTCGCCTGGAGGTGGTGCGCAACTGCATCTCCTACGTGTTTGAAGGGAAAATGCTTGAGGCAAAGAAGT TGCTTCCAGCTGTTCTCAGGGCCCTGAAGGGGCGAGCTGCCCGCCGCTGCCTCGCCCATGAGCTTCACCT GCACGTACAGCAGAACCGTGCAGTTCTGGATCATCAGCAGTTTGACTTTGTCGTCCGCATGATGAATTGC TGCCTGCAGGATTGCACTTCTCTGGATGAGCACGGCATTGCGGCTGCACTGCTGCCTTTGGTCACAGCCT TCTGCAGGAAGCTGAGCCCAGGGGTAACGCAGTTTGCATATAGCTGTGTTCAGGAGCATGTAGTGTGGAG CACACCACAGTTCTGGGAGGCCATGTTCTATGGGGATGTGCAGACCCATATCCGGGCCCTCTACCTGGAG CCTTCTGATGGCGTGAGCCCCACCCAGGAGACTGGGGAGGCACAGTCTCAGGATGATGAACGATCTGCCC TGGATGTGGCTTCAGAGCAGAGGCGCCTATGGCCAACCCTGAGCCGTGAGAAGCAGCAGGAACTGGTACA GAAGGAGGAAAGCACTGTGTTCAGCCAGGCCATCCACTATGCCAACCGCATGAGCTACCTTCTGCTGCCT CTGGACAGCAGCAAGAGCCGGCTGCTGCGGGAGCGGGCAGGGTTGGGAGACCTGGAGAGTGCCAGCAACA GCCTGGTCACCAACAGCATGGCAGGCAGTGTGGCTGAGAGCTATGACACAGAGAGCGGCTTTGAAGACGC AGAGACATGCGATGTGGCTGGGGCTGTGGTCCGCTTCATAAACCGCTTTGTGGACAAGGTCTGCACAGAG AGTGGGGTCACCAGCGACCACCTCAAAGGACTGCATGTCATGGTGCCAGACATTGTCCAGATGCACATTG AGACCCTGGAGGCTGTACACCGTGAGAGCAAGAGGCTGCCCCCAATACAGAAGCCCAAGCTGCTGAGGCC ACGCCTATTGCCTGGTGAAGAGTGTGTCCTCGATGGCCTTCGGGTGTACCTGCTGCCAGATGGGCGTGAG GAGGGTGTAGGAGGCAGTGGAGGGGGCCCTGCTCTACTTCCAGCTGAGGGTGCTGTCTTCCTTACCACAT ACCGCGTCATCTTCACGGGGATGCCTACTGACCCCCTGGTGGGGGAGCAGGTGGTTGTCCGCTCCTTCCC CGTGGCTGCGTTGACCAAGGAGAAGCGCATTAGTGTGCAGACCCCTGTGGACCAGCTTCTGCAGGACGGG CTGCAGCTTCGTTCCTGCACATTCCAGCTGCTGAAAATGGCCTTTGATGAGGAGGTGGGATCTGACAGTG CTGAGCTCTTCCGAAAGCAGCTCCACAAGCTTCGGTACCCACCAGACATCAGGGCCACCTTTGCATTCAC ACTTGGCTCAGCTCTCACACCTGGCAGGCCGCCACGAGTTACCAAGGACAAGGGTCCTTCATTCAGAACC CTGTCCAGGAACCTGGTGAAGAATGCTAAAAAGACCATTGGGCGGCAGTATGTTACTCGTAAGAAATATA ACCCCCCTGGCTGGGAACATCGGGGCCAGCCACCCCCTGAGGACCAGGAGGACGAGATATCAGTGTCAGA GGAGCTGGAGCCCAGCACACTGACCCCGTCCTCAGCCCTGAAGCCCTCTGACCGCATGACCATGAGCAGT CTGGTGGAACGGGCATGTTGCCGTGACTACCAGCGTCTTGGACTAGGTACCCTGAGCAGCAGCCTGAGCC GGGCCAAGTCTGAGCCCTTTCGCATCTCTCCTGTTAATCGCATGTATGCCATATGTCGCAGCTATCCAGG ATTGCTGATTGTTCCCCAGAGCATCCAGGACAATGCCCTGCAACGCGTGTCCCGTTGCTACCGCCAGAAC CGTTTCCCTGTGGTCTGCTGGCGCAGTGGGCGCTCCAAGGCTGTGTTGCTACGCTCTGGGGGCCTGCATG GCAAAGGTGTTGTTGGTCTCTTCAAGGCCCAGAATACACCTTCTCCAGGCCAGGCCCAGGCTGACTCCAG CAGCCTAGAGCAAGAGAAGTACCTGCAGGCTGTGGTTAGCTCCATGCCACGTTATGCTGACTCATCAGGA CGAAACACACTTAGTAGCTTCTCCTCAGCCCACATGGGTGGTCACGTGCCCAGTCCCCGAGCCAGGGTCA CCACGCTGTCCAACCCTTTGGCGGCCTCGGCCTCCAGATGGACTGCGTCCCGAGGTAAGTGGAGCAGTGT CCGAGCCAGTGGTCGAAGCAGTGGGCTTGGTGCTGATGTGGGCTCTCGGCTGGCTGGCAGAGACCTCCTC AGTACTCCCCACACCAACGGAGCGCCACCTGATTCTGGTTTTCTGCGGCCGCAGCGTGCAGCCCTCTACA TCATTGGTGACAAAGCTCAGCTCAAGGGTGTGCGCCCAGACCCTTTGCAACAGTGGGAGCTGGTTCCTAT TGAAGTATTTGAGGCAAGGCAGGTGAAAGCCAGCTTCAAGAAATTGTTGAAGGCCTGTGTGCCTGGTTGC CCTGCCACTGAGCCCAGCCCAGCCTCCTTTCTGCGGTCACTGGAGGACTCAGAGTGGCTGATCCAGATAC ACAAGCTGCTGCAGATATCGGTGCTGGTGGTGGAGCTGCTAGACTCAGGCTCCTCTGTCCTTGTGAGCCT GGAGGATGGCTGGGACATCACCACTCAGGTGGTATCCTTGGTGCAGCTCCTCTCAGACCCCTTCTACCGC ACTCTGGAAGGCTTCCGCCTGCTAGTGGAAAAGGAGTGGCTGTCCTTTGGCCATCGTTTCAGCCACCGTG GGGCCCACACCCTAGCTGGGCAGAGCAGTGGCTTCACACCCGTCTTCCTGCAGTTCCTGGACTGTGTACA CCAGGTCCATTTGCAGTTCCCCATGGAGTTTGAGTTCAGTCAGTTCTACCTCAAGTTCCTTGGTTATCAC CACACATCCCGCCGTTTCCGGACCTTCTTGCTGGACTCTGATTATGAGCGTATTGAGCTGGGACTGCTGT ATGAGGAGAAGGGTGAGCGCAGAGGCCAGTTGGCTTGCAAGTCTGTGTGGGAATATGTAGACCGGCTAAG CAAGAGGACCCCCATGTTCTACAACTACACATATGCACCTGAGGACACAGAGGTGCTGCGGCCCTACAGC AATGTGTCCAACCTGAAGGTGTGGGATTTCTACACTGAGGAGACGCTGGCTGAGGGGCCCCCTTATGACT GGGAGCTGGCCCAGGGGCCTCCTGAGCCTCCAGAAGAGGAGCGTCCTGATGGAGGTGCTCCCCAGAGCAG GCGCCGTGTGGTTTGGCCGTGCTATGACAGCCGCCCTCGAGTCCAGCCTGATGCCATCTCACGTCTGCTG GAGGAGCTGCAGCGATTGGAGACAGAGCTGGGACGGCCTTCTGAACGCTGGAAAGAGACCTGGGACCGAG TGAAAGCTGCTCAGCGCCTTGAAGGCCGGCAAGATGGACGTGGTACCCCTAGCTCATTACTGGTGTCAGC TGTGCCCCACCACCGCCGCTCGCTAGGGGTCTATCTACAGGAGGGGCCTGTGGGCTCTACTCTTAGCCTC AGCCTGGACAGTGACCAGAGCAGTGGCTCAACCACATCCAGCTCTCGTCAGGCTGCCCGACGGAGCACCA GCACGCTGTATAGCCAATTCCAGACAGCGGAGAGTGAGAACAGGTCCTATGAGGGCACCCTGTACAAGAA GGGAGCCTTCATGAAGCCCTGGAAAGCCCGTTGGTTTGTCCTGGACAAGACCAAGCACCAGCTGCGTTAC TATGACCACCGAGTGGACACAGAATGCAAGGGTGTCATTGACCTGGCAGAGGTGGAAGCTGTGGCACCTG GCACACCCACCATAGGTGCCCCTAAGACTGTGGATGAGAAGGCCTTCTTCGATGTGAAGACAACACGTCG TGTTTACAACTTCTGTGCCCAGGATGTGCCCTCAGCCCAGCAGTGGGTGGACCGGATCCAGAGCTGCCTG TCGGATGCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217707 representing NM_001271173 Red=Cloning site Green=Tags(s) MARLADYFVLVAFGPHPRGSGEGQGQILQRFPEKDWEDNPFPQGIELFCQPSGWQLCPERNPPTFFVAVL TDINSERHYCACLTFWEPVESTQEVMCTEDATEKEEEAHGGGQAQLSSTAPAQPGQLFAPKTLVLVSRLD HADVFRNSLGLIYTIHVEGLNVNLENVIGNLLTCTVPLAGGSQRTISLGAGDRQVIQTPLVDSLPVSRCS VALLFRQLGITNVLSLFCAALTEHKVLFLSRSYQRLADACRGLLALLFPLRYSFTYVPILPAQLLEVLST PTPFIIGVNAAFQAETQELLDVIVADLDGGTVTVPECVHIPPLPEPLQSQTHNVLSMVLDPELELADLAF PPPTTSASSLKMQDKELRAVFLRLFAQLLQGYRWCLHIVRIHPEPVIRFHKAAFLGQRGLVEDDFLMKVL EGMAFAGFVSERGVPYRSTDLFDELVAHEVARMRADENHPHRVLRHVQELAEQLYKNENPYPAVAMHKVQ RPGEASHLRRTHRPFPRLDEGTVQWIVDQAAAKMQGAPPAVKAERRTTVPSGPPMTAILERCSGPHINSA RRLEVVRNCISYVFEGKMLEAKKLLPAVLRALKGRAARRCLAHELHLHVQQNRAVLDHQQFDFVVRMMNC CLQDCTSLDEHGIAAALLPLVTAFCRKLSPGVTQFAYSCVQEHVVWSTPQFWEAMFYGDVQTHIRALYLE PSDGVSPTQETGEAQSQDDERSALDVASEQRRLWPTLSREKQQELVQKEESTVFSQAIHYANRMSYLLLP LDSSKSRLLRERAGLGDLESASNSLVTNSMAGSVAESYDTESGFEDAETCDVAGAVVRFINRFVDKVCTE SGVTSDHLKGLHVMVPDIVQMHIETLEAVHRESKRLPPIQKPKLLRPRLLPGEECVLDGLRVYLLPDGRE EGVGGSGGGPALLPAEGAVFLTTYRVIFTGMPTDPLVGEQVVVRSFPVAALTKEKRISVQTPVDQLLQDG LQLRSCTFQLLKMAFDEEVGSDSAELFRKQLHKLRYPPDIRATFAFTLGSALTPGRPPRVTKDKGPSFRT LSRNLVKNAKKTIGRQYVTRKKYNPPGWEHRGQPPPEDQEDEISVSEELEPSTLTPSSALKPSDRMTMSS LVERACCRDYQRLGLGTLSSSLSRAKSEPFRISPVNRMYAICRSYPGLLIVPQSIQDNALQRVSRCYRQN RFPVVCWRSGRSKAVLLRSGGLHGKGVVGLFKAQNTPSPGQAQADSSSLEQEKYLQAVVSSMPRYADSSG RNTLSSFSSAHMGGHVPSPRARVTTLSNPLAASASRWTASRGKWSSVRASGRSSGLGADVGSRLAGRDLL STPHTNGAPPDSGFLRPQRAALYIIGDKAQLKGVRPDPLQQWELVPIEVFEARQVKASFKKLLKACVPGC PATEPSPASFLRSLEDSEWLIQIHKLLQISVLVVELLDSGSSVLVSLEDGWDITTQVVSLVQLLSDPFYR TLEGFRLLVEKEWLSFGHRFSHRGAHTLAGQSSGFTPVFLQFLDCVHQVHLQFPMEFEFSQFYLKFLGYH HTSRRFRTFLLDSDYERIELGLLYEEKGERRGQLACKSVWEYVDRLSKRTPMFYNYTYAPEDTEVLRPYS NVSNLKVWDFYTEETLAEGPPYDWELAQGPPEPPEEERPDGGAPQSRRRVVWPCYDSRPRVQPDAISRLL EELQRLETELGRPSERWKETWDRVKAAQRLEGRQDGRGTPSSLLVSAVPHHRRSLGVYLQEGPVGSTLSL SLDSDQSSGSTTSSSRQAARRSTSTLYSQFQTAESENRSYEGTLYKKGAFMKPWKARWFVLDKTKHQLRY YDHRVDTECKGVIDLAEVEAVAPGTPTIGAPKTVDEKAFFDVKTTRRVYNFCAQDVPSAQQWVDRIQSCL SDA SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271173
ORF Size	5679 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271173.1, NP_001258102.1
RefSeq Size	6155 bp
RefSeq ORF	5682 bp
Locus ID	300147
MW	211.4 kDa

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