EID2 (NM_153232) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CRI2; EID-2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_153232, the custom clone sequence may differ by one or more nucleotides ATGTCCAAGCTGCCCGCAGACAGCAGTGTCCCGCAGACAGGCGCGGCGAATGGTGACAGAGACGTCCCGC AGGCGGAGGTAGGCCGCGGGAGGCGGGAGCCGGCCCCGGCACAGCCTGAGGAGGCCGGGGAAGGCGCGAT GGCGGCGGCCAGGGGAGGCCCGGTGCCGGCGGCCAGGGAAGGTCGGATGGCGGCGGCCAGGGCAGCCCCG GCGGCGGCAGCCAGGGGAGCCCCGGTGGCAGCGGCGGCGTTGGCCAGGGCAGCCGCGGCGGGCAGGGAAA GCCCGGCGGCGGCGGCGGCCAGGGAAGCCCGGATGGCGGAGGTCGCCCGCTTGCTGGGGGAGCCAGTGGA CGAAGAGGGTCCCGAGGGCAGGCCCAGGTCCAGACACGGGAACGGAGGCCTGGCTGCGTTGCCCTATCTC CGTCTCCGCCACCCACTTAGCGTTTTAGGCATCAATTACCAGCAGTTTCTCCGCCACTATCTGGAAAATT ACCCGATTGCTCCCGGCAGAATACAAGAGCTTGAAGAACGCCGCAGGCGATTCGTGGAAGCCTGCAGAGC AAGGGAAGCAGCGTTTGATGCCGAATATCAGCGAAATCCTCACAGGGTGGACCTCGATATTTTAACCTTT ACGATAGCTCTGACTGCCTCTGAAGTTATCAACCCTCTGATAGAAGAACTTGGTTGCGATAAGTTTATCA ATAGAGAATAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_153232 unedited GATTTTGTATCCGACTCCTATGGGCGGCCGCGAATTCGGCACCAGGCCGGGCATCGCTCT GTCCAGTTATTCCAGTTTCTCTGGGAGCAGCCGAGTTGACCCCACGGTCTGAGATGTCCA AGCTGCCCGCAGACAGCAGTGTCCCGCAGACAGGCGCGGCGAATGGTGACAGAGACGTGC CGCAGGCGGAGGTAGGCCGCGGGAGGCGGGAGCCGGCCCCGGCACAGCCTGAGGAGGCCG GGGAAGGCGCGATGGCGGCGGCCAGGGGAGGCCCGGTGCCGGCGGCCAGGGCAGGTCGGA TGGCGGCGGCCAGGGCAGCCCCGGCGGCGGCAGCCAGGGGAGCCCCGGTGGCAGCGGCGG CGTTGGCCAGGGCAGCCGCGGCGGGCAGGGAAAGCCCGGCGGCGGCGGCGGCCAGGGAAG CCCGGATGGCGGAGGTCGCCCGCTTGCTGGGGGAGCCAGTGGACGAAGAGGGTCCCGAGG GCAGGCCCAGGTCCAGACACGGGAACGGAGGCCTGGCTGCGTTGCCCTATCTCCGTCTCC GCCACCCACTTAGCGTTTTAGGCATCAATTACCAGCAGTTTCTCCGCCACTATCTGGAAA ATTACCCGATTGCTCCCGGCAGAATACAAGAGCTTGAAGAACGCCGCAGGCGATTCGTGG AAGCCTGCAGAGCAAGGGAAGCAGCGTTTGATGCCGAATATCAGCGAAATCCTCACAGGG TGGACCTCGATATTTTAACCTTTACGATAGCTCTGACTGCCTCTGGAGTTATCAACCCTC TGATAGAAGAACTTGCTTGCGATAAGTTTATCAATAGAGGATAATTATGTGGTGACACTA CTTCAGAGAAACTCTGCATTCATTCATACCAATCCTGCAACTTGTATTTGAGAAGTCAAG AGTATATCCGCGATAGAACGGGCCAGGTGGGAGGGAAAAAAGGGGAAAGCGGACGTTATC TTGGAAAGCTCCTCAATTGGGGAAAGTGGTAAGCTTCACGCGGAAGCTCTTGGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_153232 unedited NNAAAGATACTTTATGNNACCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTT TGACGTTCTTGAAAACTGATTTATCACATAATATATACATTTACTTTGCTTGTCACTTGA AATGCCTAAGAAAACACAAAATCAATCTTAAACTAGAAACTTCTATTGAATATGACATGA AGAGAGCACATATTCCGACAATTTCAAGTTCTTTTCATAGCCTTCCAGTCCTAATAACTC AGCGTTAACATCTTATTCTGTTCAGCACTATTTGATAATTTCTTTCATCCTCATGGGCTC TAGTATTGATGTTTCATCAGTCTCAATTTTATAAAACCATCCTTTACTTTGTAATAAAGC AATGCTGAGAGACATTTTCCATAATGCAGGGGGAAAAATGTGGATCAATCACAAACTCAA AGAACGTTACAGTTATAATGCTTTCGACATTTTTTTCTACTTCTGTGATGCTTTTTCAAG ATAAGCTTCCCCCTCCCCCTTTTTTTCCCCTCCACCTGTGCACTGTCTTATCGCGATATA CTCTTGACTTCTGAAAATCAAGTTGCAGGATTGGTATGACTGGAATGCAGAGGTTCTCTT GAAGTAGTGTCACCACATAACTATTCTCTATTGATAAACTTATCGCAACCAAGTTCTTCT ATCAGAGGGTTGATAACTTCAGAGGCAGTCAGAGCTATCGTAAAGGTTAAAATATCGAGG TCCACCCTGTGAGGATTTCGCTGATATTCGGCATCAAACGCTGCTTTCCTTGCTCTGCAA GCTTCCACGAATCGCCTGCNGCGTTCTTCAAGCTCTTGTATTCTGCCGGGAGCAATCGGG TAATTTTCCAGT
Restriction Sites	NotI-NotI
ACCN	NM_153232
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_153232.3, NP_694964.3
RefSeq Size	1409 bp
RefSeq ORF	711 bp
Locus ID	163126
UniProt ID	Q8N6I1
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	Interacts with EP300 and acts as a repressor of MYOD-dependent transcription and muscle differentiation. Inhibits EP300 histone acetyltransferase activity. Acts as a repressor of TGFB/SMAD transcriptional responses. May act as a repressor of the TGFB/SMAD3-dependent signaling by selectively blocking formation of TGFB-induced SMAD3-SMAD4 complex.[UniProtKB/Swiss-Prot Function]

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