PPPDE1 (DESI2) (AK092419) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092419, the custom clone sequence may differ by one or more nucleotides TGTATTTTTAGTAGAGATGAGGTTTCACTATGTTGGCCAGGCTGGTCTCGAACTGCTGACCTCAAGTGAT CCACCTACCTCTGCCTCCCAAAGTGCTGGGATTACAGGCATAAGATACCACCTCACCTGCCCGGCCTGTT TGGCAGTGTTTTTAAGAGCAGATGTAGTTCATATACATTCTTTTTTGTTTGTTTTGTTTTTGAGATGGAA TCTCACTTTGTCATCCAGGCTGTAGTGCAGTGGCACAGTCTTGGCTCTCTGCAGCTTCAACCTCCTGGGC CCAGGCAATCCCCCTGCCCTAGCCTCCCAAGTAGCTGAGGCTACAGGCACACACCACCATACCCAGCAAA TTTGTGTGTGTGTAAACAAGGTCTTACTATGTTGCCCAGGGTGGTCTTGAACTCCTGGTCTCAAGTAATC CTCCTGCCTTGGACTTCCAAAGTACAGAGATTACAGGCATGAGCCACCATGCCTGGCCTCATGTACACTT TTTTTTTTTTTTGAGACAGAGTCTTGCTCTGTTGCCCCGGCTGGAGTGCAGTGGTGCGATCTCGGCTCGC TGCAAGCTCCGCCTCCCGGATTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGTG CCCGCCACCACGCCTGGCTAATTTTTTGTATGTTTGGTAGAGATGGGGTTTCACTGTGTTGGCCAGGATG GTCTCGATCTCCTGACCTCATGATCTGCCCGCCTTGGCCTCCCAAAGTGCTGGGAATTACAGGTGTGAGC CACCGCGCTGGGCCGAAATATTTTTAAGTGTCTACCTCTCTGTACTCACAGGCAGTGTGATAGGTGCTTG GTAATCAAAAGTGGTTAGCACATGCTACCTTCCCTCAAGTGAGACCATCTGACTTTCCCTATAGTTAAGA TAGAGACATACTATTTAAAATAAATGTAATAAGCATTAAGAGGTAGGGCTAAACTTTTAGGGAGAAAAGG TACACGTTGTATAATTATAGGTGCCAACTTATTAAATTTTAGCTAATTTTTAGACATAAGGTACTGTGAA ACCCCTTTCCAAAGTTATTACCTAAGATATGCAAATATCTAAGATGGTACTTGGAGATCACATGATCATA ATCTTTGGTATAATTGGAGGCAAAAACAACAAAGTGGCAGTGGGGCATGAACGGCAGCCCAGTCCATTGT CTGCACAAGAGAAAAAGGATAATAAAGAATAAAGTTAGGGGCACAGAATAGACCCAGTTCTTACTATTTG AAATAGGAGTGGGGGGAAGGGTTCAAGAGTCCTAAGGACAGTATAAGGACAGGAAGTGGGCAACTGTAGA AGCAGCAGATCATCAGCGGGCAGGGATTCCTCCTGGCACTTGTTTCTATCCCGCTAGTGTCTTCAGACTG AAGCATGGCCAGGCTTCCATGCCTTGTCTGACTCTAGGCCTACTTCTGCAGAGGAGTCACAGAGCCAGGT CGTCATCTGGGAAAACACTTTGTGTGTGTGTGTGAGACGGAGTCTCACTCTGTTGCCTAGGCTGGAGTGC AGTGGCGTGATGTCAGCTCACTGCAACCTCCGCCTCCCGGGTTCAGGTGATTCTCCTGCCCCAGCCTCCC GAGTATCTGGGAATACAGGTGTGCACCACCATGCCCGGCTAATTTTTTGTATTTTTGGTAGAGACAGGGT TTCACCATGCTGGCCAGGCTGGTCTCGAACTCCTGACCTCGTAATTCACCGGCCTCAGCCTCCCAAAGTG CTGGTATTACAGGTGTGAGCCACCGCGCCTGGCCCACACTTTTTTAATTTGAGACACTCTTATCACCCAG GCTGTTTTTTCTGGACCAGGATGGGCCCCTTGAAACAAGTGGGTCAGGGAGAGAAGTAAAGGGGTAAAAT GTAGACCAGGAGAAATGGAGTGATAGACACTGGAGGAAAGGGGAAAGTCAAAAAGAATCTGTGAAAAGGA AGAATTCACAAATAGGAAAATATCCAAGATTGACTCTTCCAATTGCCTGGGGCTTCTATTACTCTAATAA TATATTGTATTAACAAGCGTCTTCTGTGTGTTCAAAGTACTTTTCAGTGTGTCTCACAGCAGTTGCAGAG ACCAAAGAAAGGAGCCAAGTGAAATTAGCTTGATTTCACAGATATGAAAAGTAAGCAACTTGACAAGGTT CTTTAGAAAGGAAGGAATGGAGCCAACAGTAGGGAGCCCAGGTTCTGAGTAAAGAGCTGCTTGTTATCAA AAGCCTGGAGTGACACCACTGAAGGCTAGTCTGCCGTGGCTTCTCTGTACGCCGCCCCCCTCCTCCCACC CTCCACTCCCTGGCAGTGGACCAGGTAATCTTAACCTCTCTTACCTAATACAGAACTCTTTCTCTTCTGT AGATTCTTTGTGGGAAAGAGATTCCTCGCTGGATCAATCGACTTGCCTACTTCAGCTCCTGTATACCCTT TCTACAGAGTTGCCTCCCGAAGGAGTGGCTCACGCCCGCAGCCCTGCAGTCTAGTGTCAGCCAAGAACTC CAGGATGAACTGGAGGAAGCAGAGGATGCTGCCGCATCCGCTTCCGTGGCAAGCACTGCAGCAGGCTCCA GACCCGGGCGCCACACTAAACTATAAATGTCTCCAAAGTCACACATTCAGAACTGTCTCTGGCAGTCGAA TATCACTAGAGAAAAGTAAACAGAGAAGCATCCTTTAGATATTTTGTATGCAAAGATGGCTCTCCCCCAA ATCCCAGTTTTTCAGCTCAGGATTATATTTGTAATC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092419 unedited GGTTCACATTAGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTTTGGCAG TGTTTTTAAGAGCAGATGTAGTTCATATACATTCTTTTTTGTTTGTTTTGTTTTTGAGAT GGAATCTCACTTTGTCATCCAGGCTGTAGTGCAGTGGCGCGGTCTTGGCTCTCTGCAGCT TCAACCTCCTGGGCCCAGGCAATCCCCCTGCCCTAGCCTCCCAAGTAGCTGAGGCTACAG GCACACACCACCATACCCAGCAAATTTGTGTGTGTGTAAACAAGGTCTTACTATGTTGCC CAGGGTGGTCTTGAACTCCTGGTCTCAAGTAATCCTCCTGCCTTGGACTTCCAAAGTACA GAGATTACAGGCATGAGCCACCATGCCTGGCCTCATGTACACTTTTTTTTTTTTTTGAGA CAGAGTCTTGCTCTGTTGCCCCGGCTGGAGTGCAGTGGTGCGATCTCGGCTCACTGCAAG CTCCGCCTCCCAGATTCACGCCATTCTCCTGCCTCGGCCTCCCGAGTGGCTGGGACTACA GGTGCCCGCCACCACGCCTAGCTAATTTTTTGTATGTTTAGTAGAGATGGGGTTTCACTG TGTTAGCCAGGATGGTCTCGATCTCCTGACCTCATGATCTGCCCGCCTCGGCCTCCCAAG TGCTGGGAATTACAGGTGTGAGCCACCGCGCTGGGCCGAAATATTTTTAAGTGTCTACCT CTCTGTACTCACAGGCAGTGTGATAGGTGCCTGGTAATCAAAAGTGGTTAGCACATGCTA CCCTCTCCTCAGTGAGACCATCAGACGTTNTCTCTTTTGGACTTTTTCCTATATGTTAAC AATGATACCTACTTTTTAAAAATACATGTAATAAGCCACTAAAGAGGTANGGGCTTAAAC TTTTAGGGAGAAAAAGACCACCCTTGATTATATTAAGGGGCCCACTTATTACTATTTACC TAATNTTTAAACATAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK092419 unedited TTTACTGTGNACCGCGGCCCGCTTNCTAGGATCGAGTTTTTTTTTTTTTTTTTTGATTAC AAATATAATCCTGAGCTGAAAAACTGGGATTTGGGGGAGAGCCATCTTTGCATACAAAAT ATCTAAAGGATGCTTCTCTGTTTACTTTTCTCTAGTGATATTCGACTGCCAGAGACAGTT CTGAATGTGTGACTTTGGAGACATTTATAGTTTAGTGTGGCGCCCGGGTCTGGAGCCTGC TGCAGTGCTTGCCACGGAAGCGGATGCGGCAGCATCCTCTGCTTCCTCCAGTTCATCCTG GAGTTCTTGGCTGACACTAGACTGCAGGGCTGCGGGCGTGAGCCACTCCTTCGGGAGGCA ACTCTGTAGAAAGGGTATACAGGAGCTGAAGTAGGCAAGTCGATTGATCCAGCGAGGAAT CTCTTTCCCACAAAGAATCTACAGAAGAGAAAGAGTTCTGTATTAGGTAAGAGAGGTTAA GATTACCTGGTCCACTGCCAGGGAGTGGAGGGTGGGAGGAGGGGGGCGGCGTACANAGAA GCCACGGCAGACTAGCCTTCAGTGGTGTCACTCCAGGCTTTTGATAACAAGCAGCTCTTT ACTCAGGACCTGGGCTCCCTACTGTTGGCTCCATTCCTTCCTTTCTAAAGAACCTTGTCA AGTTGCTTACTTTTCATATCTGTGAAATCAAGCTAATTTCACTTGGCTCCTTTCTTTGAT CTCTGCAACTGCTGTGAGACACACTGAANAGTACTTTGAACACCAGAAGACGCTTGTTAA TACATATATTATTAGAGTAATAGAAGCCCCCAGCAATTGGAAGATCAATCTTGGAATTTT CCTATTTGTGGATTTCTCTTTTTCACAGATTCTTTTTGACTTTCCCCTTTTCTCAGTGTC TATCACTCCATTTCTCCCTGTCTAC
Restriction Sites	NotI-NotI
ACCN	AK092419
Insert Size	2700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK092419.1
RefSeq Size	2766 bp
RefSeq ORF	2766 bp
Locus ID	51029
Gene Summary	Has deubiquitinating activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Deubiquitinates 'Lys-48'-linked polyubiquitination of RPS7 leading to its stabilization (PubMed:28483520).[UniProtKB/Swiss-Prot Function]

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