CIRBP (AK094781) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CIRP
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC103799 sequence for AK094781 edited (data generated by NextGen Sequencing) ATGAGCAGTCGCTGGAGCAGGTCTTCTCAAAGTACGGACAGATCTCTGAAGTCTGTAGAT GGACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCCCGTGGGTAC CGTGGTGGCTCTGCCGGGGGCCGGGGCTTCTTCCGTGGGGGCCGAGGACGGGGCCGTGGG TTCTCTAGAGGAGGAGGGGACCGAGGCTATGGGGGGAACCGGTTCGAGTCCAGGAGTGGG GGCTACGGAGGCTCCAGAGACTACTATAGCAGCCGGAGTCAGAGTGGTGGCTACAGTGAC CGGAGCTCGGGCGGGTCCTACAGAGACAGTTACGACAGTTACGGTAAGTCACACTCCGAG GGCGCCACGCTGCTGTGGCCTGCGGTGGGAGCTCGGTTCACCTTGGTGCCCTCTCCAAGC ACTTTAGGCTGGACACTCAGACCTTGTCACTGTGCTTGCCCAGAAGAGGCGCATCTGTCC TCTCAGAGCCATTTCTATCGCAGGACGCAAAAGCCAAATGAGACTGACCAAAAAGGCAAG GGAGAGCGAGGGCCCGCTGGGCAGTCAGCTAGGTGCATGTGTGGCCGCAGGCCAGCCTCC CTCGGCTGTGGGGGGTGGTTGCTCCCCGGCCGCAGGCCGCGCCCTGGTCTGGCCTCTGGG GTGAAGCTGCCTCTTGTTGCTTCGGTGCCTTTACACTGTGCCTGCTTCTTGTCCTCAGCT ACACACAACGAGTAA Clone variation with respect to AK094781.1 333 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK094781 unedited GTTACACATTTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGGCTCGGG TCGTTGTGGTGCGCTGTCTTCCCGCTTGCGTCAGGGACCTGCCCGACTCAGTGGCCGCCA TGGCATCAGATGAAGGCAAACTTTTTGTTGGAGGGCTGAGTTTTGACACCAATGAGCAGT CGCTGGAGCAGGTCTTCTCAAAGTACGGACAGATCTCTGAAGTGGTGGTTGTGAAAGACA GGGAGACCCAGAGATCTCGGGGATTTGGGTTTGTCACCTTTGAGAACATTGACGACGCTA AGGATGCCATGATGGCCATGAATGGGAAGTCTGTAGATGGACGGCAGATCCGAGTAGACC AGGCAGGCAAGTCGTCAGACAACCGATCCCGTGGGTACCGTGGTGGCTCTGCCGGGGGCC GGGGCTTCTTCCGTGGGGGCCGAGGACGGGGCCGTGGGTTCTCTAGAGGAGGAGGGGACC GAGGCTATGGGGGGAACCGGTTCGAGTCCAGGAGTGGGGGCTACGGAGGCTCCAGAGACT ACTATAGCAGCCGGAGTCAGAGTGGTGGCTACAGTGACCGGAGCTCGGGCGGGTCCTACA GAGACAGTTACGACAGTTACGGTAAGTCACACTCCGAGGGCGCCACGCTGCTGTGGCCTG CGGTGGGAGCTCGGTTCACCTTGGTGCCCTTCTCAGCACTTTAGCTGGACACTCAGACCT TGTCACTGTGCTTGCCCAGAAGAGCGCATCTGTCCTCTCAGAGCCATTTCTATCTGCAGA CGCAAAAGCCAATGAGACTGACCAAAAAGCAAGGGAAAGCTAGGCCCCCTTGGCATTAAC TAGTGCATGTTTGGCCCAAGCCACCTTCCTTGGTGTGGGGGTGGTGCTCCCCGCCCAAGC CCGCCCTGGTTGGCCTTTGGGGGAGCTG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK094781 unedited GGTCCACTATGNNACCGCGGCCGCAANCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGGGGGCACACGTGGC CCTTTATTCAAATCCCACTTTCAAAGGGCTCCACATTTTTGGGGGCAAGGACTGGCCCCC CCGTAAGCTGCCGGGCCACCGAAACCCGTGCTCCAAAAAAAACTCGAGGCCCTGAACCCC CACCTTACTGGGAAAACCCCTCCTGGAAGGAGGAGGAAGGGAGGGAAAACAGGCCCGGGC TCCAGGCAAGGGACCCGGCCCTCCCGGGAGGGGCTGCCCTGGACCCAAAAAAAACCAAAA GGCCCCCCCCGGGGGGCCATAGGGGCGGGAAAACAGGACTCACTGAAACCCCCAAGTCCC CCCCGGGAAAGGGTTTAACCCCCCATGCCATTTTTCCCAAAAGCACTTACAGGGGAAAAG CCTCCTGGGGAAGGGGGTGCCTCCAGCTTTTGTTCCAGGGGAAATCCCTCCTGGGACAGT GACCCCAAAGTTATTGTTGTTTTTAGGCCACCCTTCCCCGAGTCCTGGCCGGGCCCCTTA AACCTGGCAAAGAAAAAGAGGGTTACTCCCAAAGTCCTCCCAAGGGTTCAAAATTGCCCC TCCCGGCCTCCCCCGAAACAGCCCCAGCCCCTAAGAAAGTTTAAAGCTATTCGGGGGGGT GTGAACCAAGGCTCCGTCAAAAGCCAAAATTCCCAAGGGGGGTTTGTCCCTTGGCCCACC TGTGACCCCCCCCCCCTTTGGGATGGAACTATTTGGCCCCGGGGGGGCTCTAAAAAAAAA AAATGGCCGGCCCCCCCCTCCCAAAAAACCTAATTAAAGGGGGGCCTAAAAAGTTTGGAA AAAACGGCCT
Restriction Sites	NotI-NotI
ACCN	AK094781
Insert Size	3300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK094781.1, BAG52929.1
RefSeq Size	3026 bp
RefSeq ORF	735 bp
Locus ID	1153
Gene Summary	Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3' UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.[UniProtKB/Swiss-Prot Function]

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