UBL4A (AK000405) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DX254E; DXS254E; G6PD; GDX; GET5; MDY2; TMA24; UBL4
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK000405, the custom clone sequence may differ by one or more nucleotides AAGGACGCCGTCGCGACCGCCATGCAGCTGACGGTGAAGGCGCTGCAGGGCCGCGAGTGCAGCCTGCAGG TGCCAGAGGACGAGCTGGTGTCCACGCTGAAGCAGCTGGTCTCCGAGAAGCTGAACGTCCCAGTGCGCCA GCAGCGGCTGCTGTTCAAGGGCAAGGCCCTGGCAGATGGGAAACGACTCTCGGATTATAGCATCGGGCCC AACTCCAAGCTCAACCTAGTGGTCAAACCCCTGGAGAAGGTGCTACTAGAAGAAGGCGAGGCCCAGAGGC TGGCCGACTCCCCACCCCCGCAGGTCTGGCAGCTGATCTCCAAAGTCTTGGCCCGCCACTTCAGTGCGGC AGATGCCAGCAGGGTCCTGGAACAGCTACAGAGGGATTACGAGAGGTCCCTGAGTCGCCTGACGCTGGAC GACATCGAACGGTTGGCCAGCCGCTTCCTGCACCCTGAAGTGACTGAGACAATGGAGAAGGGCTTCTCCA AATAGAATTCTCGGAGCATGGGGAGGTGCCCAACGCCAGGCTACCGCTGCATGTCGCACTAAGTGTGTTC TCCTGTTGCAGTTGGGCTCATCATCGTCATAGCTGGCATGTACCTGGCTCTGGCCAGGTGCTAGGCACTC CTCACAGCTTGACCTGGGTTTGCTTCCACACCCTCAGAAGAGGAGAGCCGGCACAGCAGAGGCACCGGCG ATGAAGTGGCACAGCCCCAGTCGGAATCCAGCGGCTTCTGAAAGTGCCTTGGTGTGAGAAGGAGGAAGGG GCCGCTTGGAGAGCTGGGCTCTCGCATGTCATGTTTAGCCCACTGAGAATATACCCTCAGGTGACTCCCT CCCGATCCTGAAAGGGAAAGCAGCTGTCACCTGACTTCTGGCGGGTCCAACATAGCCCTCAGCTTACCTC TGCAGGAGGCCGAGTGACAGCCAGCCCTGGAACCCCCCGACCCCCGCAGCTGCTGCAGCCAGTGTGCCAG TGTGCGTTCGACAAATGGAAAAGCAGATTGGGGCCAGGGAGTCAGCAAGGCACCCCAGCTCTGTGATGTG ACTTTGGGCGAGTCTCAGTGCCACTTGGTCCCCAGCCATGGACTCTATACAGTGAGGGCCACTCACCGAC CTGAGTCAGTGTCCTCTGTCTCACAGGGCCCCTCTCCCTTCTGTTCAGTAAACAAACTGAAGCCAAAAAT GAAGCCGTGGCCAAGCGTGACCCAGAGATGGGGTGTCCTGGCCCTTAGTGACACAGCTCCTCTCTGGGGC ACCCTATCTGCTTGTCTCGTCCAGGAAGCGTTAGGGCTGATGGGTGACTCAGCAAGGCAGTGTCAGAGCT GGGACTGGGCGTAGGCCTTTGTCCTCATCCCCAGCACTGGCCTCCCTGTGGAAGATGAACATATCCCAGC CACCTGTGTACAGGGGCTCACTTTGTGTGCTCCTTGTTGCCTGAGAAGAAACCTTGGGGTGCCAGGGTGG GGGCAGAAGCATGGGCTGGGTTCCGGTTCATCCTCCTCCACCCTGCCGTGTGTGTGGGCACAAGAGGACA TCTAACCACCTGCTCCTTGGAGGAGGCCCCCAGGGGTGGTAGAGGCTGGAAGGAAGCCACATCAGGAGGA CGCCACTCCGGCCCTTCACCCTTGCCAAGTGAGCTGCTCACAGTGTGGTCAGGGCTGCGCGTGCTGGAGG CCCTCCTGCCTGGGCCTTGTGGGGCAAATATTGGGTCCCCAGGCTGGAAAGATGGACAGAGGCCCAATGG GTGAAGGCTTTGAAGAGCACACAGAAGCCCCTGGCCCCCCACGAGAGCTGGAGAGCCATGTATATGGCTT CAAAGCCACCTACGGCAGGGACACACTCGTGAGCATGTGTGGCCTGCAGTTCAGGTGATACATTTACCAG TGTTCTTGTTTGTGTGGTGCCAGGAAATTGATTTTGGAAAAAGTGAAATAACATTAAAGGTGAATGTGAG GCTTCTACTTTTATCCAAAAGGAGCTATATTAGCTAGGCTGTTTCTGATATCCAATCATTGGTTTAACAA TAAAGGCAATTTGTTTAATCAGTTAACGGAAATTTCTTGGCTTATGAAAGTGAAAAGTCCAGTGGTATTG GCATTGGCAGCAGGTGAGCAATTTCACCCAGTGTCTTCTGCCTCCCTCTGCGTTGGTATCTGCTACATCC CAGGCCACCACCTCCGAGGATGAAAAGATGGCTGCCTGCAGCTTCCACGGAATCCCTCCCTCACTGCCAG AGCAGCATCTTCTCTGTGTACCACCTCTGCTGTCTCAGATGCCCCCAGCAAATAAACACTCTTCTCGTTG GTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK000405 unedited NAAAGGGGAANNAAANAANNAATCACNCNCGCGCCTGTCCAGCATTTGTNATACGACTCA CTATAGGGCGGCCGCNGATTCGGCACCAGGTCGCGACCGCCATGCAGCTGACGGTGAAGG CGCTGCAGGGCCGCGAGTGCAGCCTGCAGGTGCCAGAGGACGAGCTGGTGTCCACGCTGA AGCAGCTGGTCTCCGAGAAGCTGAACGTCCCAGTGCGCCAGCAGCGGCTGCTGTTCAAGG GCAAGGCCCTGGCAGATGGGAAACGACTCTCGGATTATAGCATCGGGCCCAACTCCAAGC TCAACCTAGTGGTCAAACCCCTGGAGAAGGTGCTACTAGAAGAAGGCGAGGCCCAGAGGC TGGCCGACTCCCCACCCCCGCAGGTCTGGCAGCTGATCTCCAAAGTCTTGGCCCGCCACT TCAGTGCGGCAGATGCCAGCAGGGTCCTGGAACAGCTACAGAGGGATTACGAGAGGTCCC TGAGTCGCCTGACGCTGGACGACATCGAACGGTTGGCCAGCCGCTTCCTGCACCCTGAAG TGACTGAGACAATGGAGAAGGGCTTCTCCAAATAGAATTCTCGGAGCATGGGGAGGTGCC CAACGCCAGGCTACCGCTGCATGTCGCACTAAGTGTGTTCTCCTGTTGCAGTTGGGCTCA TCATCGTCATAGCTGGCATGTACCTGGCTCTGGCCAGGTGCTAGGCACTTCTCACAGCTT GACCTGGGTTTGCTTCCACACCCTCAGCAGAGGAGAGCCCGCACAGCAGAAGCACCGGCG ATGAAGTTGCACAGCCCCAGTCGGAATCCAGCGCCTTCTGAAGTGCCTGTTGTTGGAAAC GAAGGAAGGGCCCGCTGAAAACCTGGGCTCTCGCATGTCATGTTTACCCCCTTGAGAATA CCCTCAGGGACTCCCTCCCGATCTGAAAGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK000405 unedited AGTTAAAATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACCAACCAAAAAAATGTTT ATTTGCTGGGGGCATCTGAAACAGCAGAGGTGGTACACAAAAAAAATGCTGCTCTGGCAG TGAGGGAGGGATTCCGTGGAAACTGCAGGCAGCCATCTTTTCATCCTCGGAGGTGGTGGC CTGGGATGTAGCAAATACCAACGCAAAGGGAGGCAGAAAACACTGGGTGAAATTGCTCAC CTGCTGCCAATGCCAATACCACTGGACTTTTCACTTTCATAAGCCAAGAAATTTCCGTTA ACTGATTAAACAAATTGCCTTTATTGTTAAACCAATGATTGGATATCAAAAACAGCCTAA CTAATATAGCTCCTTTTGGATAAAAGTAGAAGCCTCACATTCACCTTTAATGTTATTTCA CTTTTTCCAAAATCAATTTCCTGGCACCACACAAACAAGAACACTGGTAAATGTATCACC TGAACTGCAGGCCACACATGCTCACGAGTGTGTCCCTGCCGTAAGTGGCTTTGAAACCAT ATACATGGCTCTCCAACTCTCGTGGGGGGCCAGGGGCTTCTGTGTGCTCTTCAAAGCCTT CACCCATTGGGCCTCTGTCCATCTTTCCAGCCTGGGGACCCAATATTTGCCCCACAAAGC CCATGCAAGAGGGCCTTCAGCACGCGCAGCCCTTGACACACTGTGAGCAGCTCACTTGCA AAGGTGAAGGGCCCGATTGGCGTCCTCCCTGAGTGGCTTNCTTCCAGCCTCTACACCCCT GGGGGCCTCCTCCAAAAACAGGGGGTTAAATGTCCCCTTGGGCCCACCACCCGCAGGGTG GGA
Restriction Sites	NotI-NotI
ACCN	AK000405
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK000405.1
RefSeq Size	2342 bp
RefSeq ORF	2342 bp
Locus ID	8266
Domains	UBQ
Protein Families	Druggable Genome
Gene Summary	As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892, PubMed:25535373). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303).[UniProtKB/Swiss-Prot Function]

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