MAP4 (NM_002375) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_002375, the custom clone sequence may differ by one or more nucleotides ATGGCTGACCTCAGTCTTGCAGATGCATTAACAGAACCATCTCCAGACATTGAGGGAGAGATAAAGCGGG ACTTCATTGCCACACTAGAGGCAGAGGCCTTTGATGATGTTGTGGGAGAAACTGTTGGAAAAACAGACTA TATTCCTCTCCTGGATGTTGATGAGAAAACCGGGAACTCAGAGTCAAAGAAGAAACCGTGCTCAGAAACT AGCCAGATTGAAGATACTCCATCTTCTAAACCAACACTCCTAGCCAATGGTGGTCATGGAGTAGAAGGGA GCGATACTACAGGGTCTCCAACTGAATTCCTTGAAGAGAAAATGGCCTACCAGGAATACCCAAATAGCCA GAACTGGCCAGAAGATACCAACTTTTGTTTCCAACCTGAGCAAGTGGTCGATCCTATCCAGACTGATCCC TTTAAGATGTACCATGATGATGACCTGGCAGATTTGGTCTTTCCCTCCAGTGCGACAGCTGATACTTCAA TATTTGCAGGACAAAATGATCCCTTGAAAGACAGTTACGGTATGTCTCCCTGCAACACAGCTGTTGTACC TCAGGGGTGGTCTGTGGAAGCCTTAAACTCTCCACACTCAGAGTCCTTTGTTTCCCCAGAGGCTGTTGCA GAACCTCCTCAGCCAACGGCAGTTCCCTTAGAGCTAGCCAAGGAGATAGAAATGGCATCAGAAGAGAGGC CACCAGCACAAGCATTGGAAATAATGATGGGACTGAAGACTACTGACATGGCACCATCTAAAGAAACAGA GATGGCCCTCGCCAAGGACATGGCACTAGCTACAAAAACCGAGGTGGCATTGGCTAAAGATATGGAATCA CCCACCAAATTAGATGTGACACTGGCCAAGGACATGCAGCCATCCATGGAATCAGATATGGCCCTAGTCA AGGACATGGAACTACCCACAGAAAAAGAAGTGGCCCTGGTTAAGGATGTCAGATGGCCCACAGAAACAGA TGTATCTTCAGCCAAGAATGTGGTACTGCCCACAGAAACAGAGGTAGCCCCAGCCAAGGATGTGACACTG TTGAAAGAAACAGAGAGGGCATCTCCTATAAAAATGGACTTAGCCCCTTCCAAGGACATGGGACCACCCA AAGAAAACAAGAAAGAAACAGAGAGGGCATCTCCTATAAAAATGGACTTGGCTCCTTCCAAGGACATGGG ACCACCCAAAGAAAACAAGATAGTCCCAGCCAAGGATTTGGTATTACTCTCAGAAATAGAGGTGGCACAG GCTAATGACATTATATCATCCACAGAAATATCCTCTGCTGAGAAGGTGGCTTTGTCCTCAGAAACAGAGG TAGCCCTGGCCAGGGACATGACACTGCCCCCGGAAACCAACGTGATCTTGACCAAGGATAAAGCACTACC TTTAGAAGCAGAGGTGGCCCCAGTCAAGGACATGGCTCAACTCCCAGAAACAGAAATAGCCCCGGCCAAG GATGTGGCTCCGTCCACAGTAAAAGAAGTGGGCTTGTTGAAGGACATGTCTCCACTATCAGAAACAGAAA TGGCTCTGGGCAAGGATGTGACTCCACCTCCAGAAACAGAAGTAGTTCTCATCAAGAACGTATGTCTGCC TCCAGAAATGGAGGTGGCCCTGACTGAGGATCAGGTCCCAGCCCTCAAAACAGAAGCACCCCTGGCTAAG GATGGGGTTCTGACCCTGGCCAACAATGTGACTCCAGCCAAAGATGTTCCACCACTCTCAGAAACAGAGG CAACACCAGTTCCAATTAAAGACATGGAAATTGCACAAACACAAAAAGGAATAAGTGAGGATTCCCATTT AGAATCTCTGCAGGATGTGGGGCAGTCAGCTGCACCTACTTTCATGATTTCACCAGAAACCGTCACAGGA ACGGGGAAAAAGTGCAGCTTGCCGGCCGAGGAGGATTCTGTGTTAGAAAAACTAGGGGAAAGGAAACCAT GCAACAGTCAACCTTCTGAGCTTTCTTCAGAGACCTCAGGAATAGCCAGGCCAGAAGAAGGAAGGCCTGT GGTGAGTGGGACAGGAAATGACATCACCACCCCACCGAACAAGGAGCTCCCACCAAGCCCAGAGAAGAAA ACAAAGCCTTTGGCCACCACTCAACCTGCAAAGACTTCAACATCGAAAGCCAAAACACAGCCCACTTCTC TCCCTAAGCAGCCAGCTCCCACCACCATTGGTGGGTTGAATAAAAAACCCATGAGCCTTGCTTCAGGCTT AGTGCCAGCTGCCCCACCCAAACGCCCTGCCGTCGCCTCTGCCAGGCCTTCCATCTTACCTTCAAAAGAC GTGAAGCCAAAGCCCATTGCAGATGCAAAGGCTCCTGAGAAGCGGGCCTCACCATCCAAGCCAGCTTCTG CCCCAGCCTCCAGATCTGGGTCCAAGAGCACTCAGACTGTTGCAAAAACCACAACAGCTGCTGCTGTTGC CTCAACTGGCCCAAGCAGTAGGAGCCCCTCCACGCTCCTGCCCAAGAAGCCCACTGCCATTAAGACTGAG GGAAAACCTGCAGAAGTCAAGAAGATGACTGCAAAGTCTGTACCAGCTGACTTGAGTCGCCCAAAGAGCA CCTCCACCAGTTCCATGAAGAAAACCACCACTCTCAGTGGGACAGCCCCCGCTGCAGGGGTGGTTCCCAG CCGAGTCAAGGCCACACCCATGCCCTCCCGGCCCTCCACAACTCCTTTCATAGACAAGAAGCCCACCTCG GCCAAACCCAGCTCCACCACCCCCCGGCTCAGCCGCCTGGCCACCAATACTTCTGCTCCTGATCTGAAGA ATGTCCGCTCCAAGGTTGGCTCCACGGAAAACATCAAGCATCAGCCTGGAGGAGGCCGGGCCAAAGTAGA GAAAAAAACAGAGGCAGCTGCTACAACCCGAAAGCCTGAATCTAATGCAGTCACTAAAACAGCCGGCCCA ATTGCAAGTGCACAGAAACAACCTGCGGGGAAAGTCCAGATAGTCTCCAAAAAAGTGAGCTACAGCCATA TTCAGTCCAAGTGTGGTTCCAAGGACAATATTAAGCATGTCCCTGGAGGTGGTAATGTTCAGATTCAGAA CAAGAAAGTGGACATCTCTAAGGTCTCCTCCAAGTGTGGGTCTAAGGCTAACATCAAGCACAAGCCTGGT GGAGGAGATGTCAAGATTGAAAGTCAGAAGTTGAACTTCAAGGAGAAGGCCCAGGCCAAGGTGGGATCCC TCGATAATGTGGGCCACCTACCTGCAGGAGGTGCTGTGAAGACTGAGGGCGGTGGCAGCGAGGCTCCTCT GTGTCCGGGTCCCCCTGCTGGGGAGGAGCCGGCCATCTCTGAGGCAGCGCCTGAAGCTGGCGCCCCCACT TCAGCCAGTGGCCTCAATGGCCACCCCACCCTGTCAGGGGGTGGTGACCAAAGGGAGGCCCAGACCTTGG ACAGCCAGATCCAGGAGACAAGCATCTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_002375 unedited AGGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCAGCTCC GTCTCGGCGGCGGCGGGCAGTTGCAGTGGTGCAGAATGGCTGACCTCAGTCTTGCAGATG CATTAACAGAACCATCTCCAGACATTGAGGGAGAGATAAAGCGGGACTTCATTGCCACAC TAGAGGCAGAGGCCTTTGATGATGTTGTGGGAGAAACTGTTGGAAAAACAGACTATATTC CTCTCCTGGATGTTGATGAGAAAACCGGGAACTCAGAGTCAAAGAAGAAACCGTGCTCAG AAACTAGCCAGATTGAAGATACTCCATCTTCTAAACCAACACTCCTAGCCAATGGTGGTC ATGGAGTAGAAGGGAGCGATACTACAGGGTCTCCAACTGAATTCCTTGAAGAGAAAATGG CCTACCAGGAATACCCAAATAGCCAGAACTGGCCAGAAGATACCAACTTTTGTTTCCAAC CTGAGCAAGTGGTCGATCCTATCCAGACTGATCCCTTTAAGATGTACCATGATGATGACC TGGCAGATTTGGTCTTTCCCTCCAGTGCGACAGCTGATACTTCAATATTTGCAGGACANA ATGATCCCTTGAAAGACAGTTACGGTATGTCTCCCTGCAACACAGCTGTTGTACCTCAGG NGTGGCCTGTGGAAGCCTTAACTCTNCACACTCAGAGTCCTTTGTTTCCCCAGAGGGCTG TGCAGAACCTNCTCAGCCAACGGCAGTTCCCTTAGAGCTANGCCAGGAGATAGAAATGCC ATCAGAAGAAGAGGCACCAGCANCAGCATTGGNNAATATGATGGGACTGAAGACTACTGA CATGGCACCATCTAAAGAAACAGAGATGCCCTCCGCCAGGACATGCACTACCTACAAAAA CCGAGGTGGCATTGGCTCAAGATATGGATCCACCCACCAATTAGATGTGACCTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_002375 unedited CTATGGACCGCGGCACGCTTCTATGATCGAGTTTTTTTTTTTTTTTTTTGGGGACATCAG GCAGCTTTATTTATTTACTCTTAAAACTGTTACAACAGAATCATGGACTGACACAGGTAA TGGCTGAGCCATAAGCAAATCGAGAAGTACAGAAATGTCCCACCCCAAACAGCTGCGGAG TACACATCACACAGGGCCTCTGGTCCCGGCCTTCTCAAGTGCTCTGGAGTGGAGGATCCT TTGAGGGAACTCTGACCACTCCTGTTGTCTACCTAAAGAGCACGCCACTTGGGCCACCTA CCCCCAACCTTTGGCCAAAGGAGTGAAAGGACCTGGAACCTGTCGTCAACCTCAGCATCC TCATGACCCCAGGACAGACGCAGTGGAGGCAGCAGGTACTCTGGGCCTGTGCTGTTGCCC CTGAGGTGTCTCCTGACTACGCAACCCTGTTTCCTTCCCACTTTCATCCCAGCAGGCCCT TTCTTCCTGCTGACAAAACACTGGCTCCCTCATGTTCTTGGCACAGCAGAAGTGTTCTGC ACATGGTCTCTACGGAGGAAAGCTGGTCCCCCTGGCCCAGGCAGCACTTCCTCCGTGAGC CCCTGCAGTTTCCTGGTGTGTGCTTGGGGAGCTAGGGACATGGTGTCAAACCTTCACAAA GCACTGCTACCTCAGATAGGGGGAGGACCTGTCAAGCCCAGGACAGCCTCCCTGCACATG GAGATCAAATCTAAACTTAAGCAAGACCCCTACCTTCTCCAGGGATGCCAGGACCANAGA ATGGCANAGGTCATGAAGGGGCATGGATGAGCCCAGTCTGGGGCACCCCTCTAAAAGGCA ATCTACACTGCAGCCAATCCCCCTCACGAAAGCCCTTGGATCTCCAAGCCTTNCACTAAA ACTTAGTTGAATCCTTTTTAAAAACCTGCAACTTAATACAAAAAACAAGGCCAAAGAACC T
Restriction Sites	NotI-NotI
ACCN	NM_002375
Insert Size	5850 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_002375.3, NP_002366.2
RefSeq Size	6337 bp
RefSeq ORF	3459 bp
Locus ID	4134
UniProt ID	P27816
Domains	tubulin-binding
Gene Summary	The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] Transcript Variant: This variant (1) represents the longest transcript. It encodes the longest isoform (1).

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