CDC45L (CDC45) (NM_003504) Human Untagged Clone
CAT#: SC122659
CDC45 (untagged)-Human cell division cycle 45 homolog (S. cerevisiae) (CDC45), transcript variant 2
CNY 4,224.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CDC45L; CDC45L2; MGORS7; PORC-PI-1 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_003504 edited
CCTCGTGCCGCCGGGCTCTTGGTACCTCAGCGCGAGCGCCAGGCGTCCGGCCGCCGTGGC TATGTTCGTGTCCGATTTCCGCAAAGAGTTCTACGAGGTGGTCCAGAGCCAGAGGGTCCT TCTCTTCGTGGCCTCGGACGTGGATGCTCTGTGTGCGTGCAAGATCCTTCAGGCCTTGTT CCAGTGTGACCACGTGCAATATACGCTGGTTCCAGTTTCTGGGTGGCAAGAACTTGAAAC TGCATTTCTTGAGCATAAAGAACAGTTTCATTATTTTATTCTCATAAACTGTGGAGCTAA TGTAGACCTATTGGATATTCTTCAACCTGATGAAGACACTATATTCTTTGTGTGTGACAC CCATAGGCCAGTCAATGTCGTCAATGTATACAACGATACCCAGATCAAATTACTCATTAA ACAAGATGATGACCTTGAAGTTCCCGCCTATGAAGACATCTTCAGGGATGAAGAGGAGGA TGAAGAGCATTCAGGAAATGACAGTGATGGGTCAGAGCCTTCTGAGAAGCGCACACGGTT AGAAGAGGAGATAGTGGAGCAAACCATGCGGAGGAGGCAGCGGCGAGAGTGGGAGGCCCG GAGAAGAGACATCCTCTTTGACTACGAGCAGTATGAATATCATGGGACATCGTCAGCCAT GGTGATGTTTGAGCTGGCTTGGATGCTGTCCAAGGACCTGAATGACATGCTGTGGTGGGC CATCGTTGGACTAACAGACCAGTGGGTGCAAGACAAGATCACTCAAATGAAATACGTGAC TGATGTTGGTGTCCTGCAGCGCCACGTTTCCCGCCACAACCACCGGAACGAGGATGAGGA GAACACACTCTCCGTGGACTGCACACGGATCTCCTTTGAGTATGACCTCCGCCTGGTGCT CTACCAGCACTGGTCCCTCCATGACAGCCTGTGCAACACCAGCTATACCGCAGCCAGGTT CAAGCTGTGGTCTGTGCATGGACAGAAGCGGCTCCAGGAGTTCCTTGCAGACATGGGTCT TCCCCTGAAGCAGGTGAAGCAGAAGTTCCAGGCCATGGACATCTCCTTGAAGGAGAATTT GCGGGAAATGATTGAAGAGTCTGCAAATAAATTTGGGATGAAGGACATGCGCGTGCAGAC TTTCAGCATTCATTTTGGGTTCAAGCACAAGTTTCTGGCCAGCGACGTGGTCTTTGCCAC CATGTCTTTGATGGAGAGCCCCGAGAAGGATGGCTCAGGGACAGATCACTTCATCCAGGC TCTGGACAGCCTCTCCAGGAGTAACCTGGACAAGCTGTACCATGGCCTGGAACTCGCCAA GAAGCAGCTGCGAGCCACCCAGCAGACCATTGCCAGCTGCCTTTGCACCAACCTCGTCAT CTCCCAGGGGCCTTTCCTGTACTGCTCTCTCATGGAGGGCACTCCAGATGTCATGCTGTT CTCTAGGCCGGCATCCCTAAGCCTGCTCAGCAAACACCTGCTCAAGTCCTTTGTGTGTTC GACAAAGAACCGGCGCTGCAAACTGCTGCCCCTGGTGATGGCTGCCCCCCTGAGCATGGA GCATGGCACAGTGACCGTGGTGGGCATCCCCCCAGAGACCGACAGCTCGGACAGGAAGAA CTTTTTTGGGAGGGCGTTTGAGAAGGCAGCGGAAAGCACCAGCTCCCGGATGCTGCACAA CCATTTTGACCTCTCAGTAATTGAGCTGAAAGCTGAGGATCGGAGCAAGTTTCTGGACGC ACTTATTTCCCTCCTGTCCTAGGAATTTGATTCTTCCAGAATGACCTTCTTATTTATGTA ACTGGCTTTCATTTAGATTGTAAGTTATGGACATGATTTGAGATGTAGAAGCCATTTTTT ATTAAATAAAATGCTTATTTTAGGCTCAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_003504 unedited
NAGATGTTCAGGTTTTGTNATACGACTCATATAGGCGGCACGCGAATTCGCACGAGGCCT CGTGCCGCCGGGCTCTTGGTACCTCAGCGCGAGCGCCAGGCGTCCGGCCGCCGTGGCTAT GTTCGTGTCCGATTTCCGCAAAGAGTTCTACGAGGTGGTCCAGAGCCAGAGGGTCCTTCT CTTCGTGGCCTCGGACGTGGATGCTCTGTGTGCGTGCAAGATCCTTCAGGCCTTGTTCCA GTGTGACCACGTGCAATATACGCTGGTTCCAGTTTCTGGGTGGCAAGAACTTGAAACTGC ATTTCTTGAGCATAAAGAACAGTTTCATTATTTTATTCTCATAAACTGTGGAGCTAATGT AGACCTATTGGATATTCTTCAACCTGATGAAGACACTATATTCTTTGTGTGTGACACCCA TAGGCCAGTCAATGTCGTCAATGTATACAACGATACCCAGATCAAATTACTCATTAAACA AGATGATGACCTTGAAGTTCCCGCCTATGAAGACATCTTCAGGGATGAAGAGGAGGATGA AGAGCATTCAAGACATGACAGTGATGGGTCAGAGCCTTCTGAGAAGCGCACACGGTTAGA AGAGGAGATAGTGGAGCAAACCTTGCGGAGGAAGCATCGGCGAGAGTGGGAAGCCCGGAG AAGAACTTCCTCTTGACTACGAGCAGTGTGATTATCAGGGGACATCGTCGGCCATGGTGA TGTTTTGACCTGGCTTGGTTGCCTGTCCAAGACCTGAATGACAGGCTGTGGTGGGCCCTC GGGGGACTAACGAACAATGAGGTGCCAGACAAGATCCCCCGATGGAATCCGGGCCGGATG TTGGGGGTCCGGCACGCGCCCGTGTTCCGCCGTAACCCCGGGAACGG |
Restriction Sites | Please inquire |
ACCN | NM_003504 |
Insert Size | 1914 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003504.3, NP_003495.1 |
RefSeq Size | 1938 bp |
RefSeq ORF | 1701 bp |
Locus ID | 8318 |
UniProt ID | O75419 |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | Cell cycle |
Gene Summary | The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202442 | CDC45 (Myc-DDK-tagged)-Human cell division cycle 45 homolog (S. cerevisiae) (CDC45), transcript variant 2 |
CNY 4,216.00 |
|
RC202442L3 | Lenti ORF clone of Human cell division cycle 45 homolog (S. cerevisiae) (CDC45), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202442L4 | Lenti ORF clone of Human cell division cycle 45 homolog (S. cerevisiae) (CDC45), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG202442 | CDC45 (tGFP-tagged) - Human cell division cycle 45 homolog (S. cerevisiae) (CDC45), transcript variant 2 |
CNY 5,816.00 |