CDC45L (CDC45) (NM_003504) Human Untagged Clone

CAT#: SC122659

CDC45 (untagged)-Human cell division cycle 45 homolog (S. cerevisiae) (CDC45), transcript variant 2



  "NM_003504" in other vectors (4)

CNY 4,224.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
CDC45 mouse monoclonal antibody,clone OTI4F3
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CDC45L; CDC45L2; MGORS7; PORC-PI-1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_003504 edited
CCTCGTGCCGCCGGGCTCTTGGTACCTCAGCGCGAGCGCCAGGCGTCCGGCCGCCGTGGC
TATGTTCGTGTCCGATTTCCGCAAAGAGTTCTACGAGGTGGTCCAGAGCCAGAGGGTCCT
TCTCTTCGTGGCCTCGGACGTGGATGCTCTGTGTGCGTGCAAGATCCTTCAGGCCTTGTT
CCAGTGTGACCACGTGCAATATACGCTGGTTCCAGTTTCTGGGTGGCAAGAACTTGAAAC
TGCATTTCTTGAGCATAAAGAACAGTTTCATTATTTTATTCTCATAAACTGTGGAGCTAA
TGTAGACCTATTGGATATTCTTCAACCTGATGAAGACACTATATTCTTTGTGTGTGACAC
CCATAGGCCAGTCAATGTCGTCAATGTATACAACGATACCCAGATCAAATTACTCATTAA
ACAAGATGATGACCTTGAAGTTCCCGCCTATGAAGACATCTTCAGGGATGAAGAGGAGGA
TGAAGAGCATTCAGGAAATGACAGTGATGGGTCAGAGCCTTCTGAGAAGCGCACACGGTT
AGAAGAGGAGATAGTGGAGCAAACCATGCGGAGGAGGCAGCGGCGAGAGTGGGAGGCCCG
GAGAAGAGACATCCTCTTTGACTACGAGCAGTATGAATATCATGGGACATCGTCAGCCAT
GGTGATGTTTGAGCTGGCTTGGATGCTGTCCAAGGACCTGAATGACATGCTGTGGTGGGC
CATCGTTGGACTAACAGACCAGTGGGTGCAAGACAAGATCACTCAAATGAAATACGTGAC
TGATGTTGGTGTCCTGCAGCGCCACGTTTCCCGCCACAACCACCGGAACGAGGATGAGGA
GAACACACTCTCCGTGGACTGCACACGGATCTCCTTTGAGTATGACCTCCGCCTGGTGCT
CTACCAGCACTGGTCCCTCCATGACAGCCTGTGCAACACCAGCTATACCGCAGCCAGGTT
CAAGCTGTGGTCTGTGCATGGACAGAAGCGGCTCCAGGAGTTCCTTGCAGACATGGGTCT
TCCCCTGAAGCAGGTGAAGCAGAAGTTCCAGGCCATGGACATCTCCTTGAAGGAGAATTT
GCGGGAAATGATTGAAGAGTCTGCAAATAAATTTGGGATGAAGGACATGCGCGTGCAGAC
TTTCAGCATTCATTTTGGGTTCAAGCACAAGTTTCTGGCCAGCGACGTGGTCTTTGCCAC
CATGTCTTTGATGGAGAGCCCCGAGAAGGATGGCTCAGGGACAGATCACTTCATCCAGGC
TCTGGACAGCCTCTCCAGGAGTAACCTGGACAAGCTGTACCATGGCCTGGAACTCGCCAA
GAAGCAGCTGCGAGCCACCCAGCAGACCATTGCCAGCTGCCTTTGCACCAACCTCGTCAT
CTCCCAGGGGCCTTTCCTGTACTGCTCTCTCATGGAGGGCACTCCAGATGTCATGCTGTT
CTCTAGGCCGGCATCCCTAAGCCTGCTCAGCAAACACCTGCTCAAGTCCTTTGTGTGTTC
GACAAAGAACCGGCGCTGCAAACTGCTGCCCCTGGTGATGGCTGCCCCCCTGAGCATGGA
GCATGGCACAGTGACCGTGGTGGGCATCCCCCCAGAGACCGACAGCTCGGACAGGAAGAA
CTTTTTTGGGAGGGCGTTTGAGAAGGCAGCGGAAAGCACCAGCTCCCGGATGCTGCACAA
CCATTTTGACCTCTCAGTAATTGAGCTGAAAGCTGAGGATCGGAGCAAGTTTCTGGACGC
ACTTATTTCCCTCCTGTCCTAGGAATTTGATTCTTCCAGAATGACCTTCTTATTTATGTA
ACTGGCTTTCATTTAGATTGTAAGTTATGGACATGATTTGAGATGTAGAAGCCATTTTTT
ATTAAATAAAATGCTTATTTTAGGCTCAAAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_003504 unedited
NAGATGTTCAGGTTTTGTNATACGACTCATATAGGCGGCACGCGAATTCGCACGAGGCCT
CGTGCCGCCGGGCTCTTGGTACCTCAGCGCGAGCGCCAGGCGTCCGGCCGCCGTGGCTAT
GTTCGTGTCCGATTTCCGCAAAGAGTTCTACGAGGTGGTCCAGAGCCAGAGGGTCCTTCT
CTTCGTGGCCTCGGACGTGGATGCTCTGTGTGCGTGCAAGATCCTTCAGGCCTTGTTCCA
GTGTGACCACGTGCAATATACGCTGGTTCCAGTTTCTGGGTGGCAAGAACTTGAAACTGC
ATTTCTTGAGCATAAAGAACAGTTTCATTATTTTATTCTCATAAACTGTGGAGCTAATGT
AGACCTATTGGATATTCTTCAACCTGATGAAGACACTATATTCTTTGTGTGTGACACCCA
TAGGCCAGTCAATGTCGTCAATGTATACAACGATACCCAGATCAAATTACTCATTAAACA
AGATGATGACCTTGAAGTTCCCGCCTATGAAGACATCTTCAGGGATGAAGAGGAGGATGA
AGAGCATTCAAGACATGACAGTGATGGGTCAGAGCCTTCTGAGAAGCGCACACGGTTAGA
AGAGGAGATAGTGGAGCAAACCTTGCGGAGGAAGCATCGGCGAGAGTGGGAAGCCCGGAG
AAGAACTTCCTCTTGACTACGAGCAGTGTGATTATCAGGGGACATCGTCGGCCATGGTGA
TGTTTTGACCTGGCTTGGTTGCCTGTCCAAGACCTGAATGACAGGCTGTGGTGGGCCCTC
GGGGGACTAACGAACAATGAGGTGCCAGACAAGATCCCCCGATGGAATCCGGGCCGGATG
TTGGGGGTCCGGCACGCGCCCGTGTTCCGCCGTAACCCCGGGAACGG
Restriction Sites Please inquire     
ACCN NM_003504
Insert Size 1914 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003504.3, NP_003495.1
RefSeq Size 1938 bp
RefSeq ORF 1701 bp
Locus ID 8318
UniProt ID O75419
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Cell cycle
Gene Summary The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Transcript Variant: This variant (2) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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