PRAS40 (AKT1S1) (BC016043) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AKT1 substrate 1 (proline-rich); Lobe; MGC2865; OTTHUMP00000196757; OTTHUMP00000196758; OTTHUMP00000196759; OTTHUMP00000196760; PRAS40; proline-rich Akt substrate, 40 kDa
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_001098632 edited GGATAACGGGATGAAGCCGTCAGTGCCCCCAGAAACGAAGCGGCCCCGGACGAATTTCTG AGTCACCGTCGCGAGAAAGCGGGCTGAGCCGCCATTTTGAAGCCTGGCAAACCGAAGCAA GAAATGCTGCCGTGTTGGATCTTTGCCAGCCTTCGTGCCGAATGGGAGCAGGGCGCGGAT GGCGTCGGGGCGCCCCGAGGAGCTGTGGGAGGCCGTGGTGGGGGCCGCTGAGCGCTTCCG GGCCCGGACTGGCACGGAGCTGGTGCTGCTGACCGCGGCCCCGCCGCCACCACCCCGCCC GGGCCCCTGTGCCTATGCTGCCCATGGTCGAGGAGCCCTGGCGGAGGCAGCGCGCCGTTG CCTCCACGACATCGCACTGGCCCACAGGGCTGCCACTGCTGCTCGGCCTCCTGCGCCCCC ACCAGCACCACAGCCACCCAGTCCCACACCCAGCCCACCCCGGCCTACCCTGGCCAGAGA GGACAACGAGGAGGACGAGGATGAGCCCACAGAGACAGAGACCTCCGGGGAGCAGCTGGG CATTAGTGATAATGGAGGGCTCTTTGTGATGGATGAGGACGCCACCCTCCAGGACCTTCC CCCCTTCTGTGAGTCAGACCCCGAGAGTACAGATGATGGCAGCCTGAGCGAGGAGACCCC CGCCGGCCCCCCCACCTGCTCAGTGCCCCCAGCCTCAGCCCTACCCACACAGCAGTACGC CAAGTCCCTGCCTGTGTCTGTGCCCGTCTGGGGCTTCAAGGAGAAGAGGACAGAGGCGCG GTCATCAGATGAGGAGAATGGGCCGCCCTCTTCGCCCGACCTGGACCGCATCGCGGCGAG CATGCGCGCGCTGGTGCTGCGAGAGGCCGAGGACACCCAGGTCTTCGGGGACCTGCCACG GCCGCGGCTTAACACCAGCGACTTCCAGAAGCTGAAGCGGAAATATTGAAGTCCAGGGAG GGAGCGCCCCGGGCCGCGTCCGCCCCGTCCCACACTACGCCCCCGCCCCACTCCCGGGGC CTGCTAATCTGAGGCCGATCCGGGACCGGCCTCCTTGCGTCTCCCATTCCCAATTGGCTC CAGCTTCCTGGGGGCGTGGCCAAGCCCTCCTCTTCCCAGAATTGGCCCGGGGCCTTCAAT TTACGTTCTTTACACTACGGGGACTGGGGTCGTCTTTGCCCACGTCCCGACAACTTGTTC CCTGACCCCCTCAGGGATGGCCCCAAACTGTCCCTGCCTCTGGCACCCCCTTTCATTGGT TCCATCCATCCCCACAACAGCCTGCCAATCGAAGCCCGTCCCTGCATCCAGGATGGTACC AGCTCCCGCCCCTCGCCCCCCACCTCCACAGGTGCCTTAAAGGGCCCTCGTCCACCCAAG GTGGGGGGCAGGGGCCCTCACTCTCCGGCCCTGGTGTGGGGGAGAGAGTGAGGGGTTGGG GGATCGGCAGTTGGGAGGGGCGCTCTGAGATTAAAGAGTTTTACCTCTGAGATAAAAAAA AAAAAAAAAAA
Restriction Sites	Please inquire
ACCN	BC016043
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC016043.1, AAH16043.1
RefSeq Size	1511 bp
Locus ID	84335
Gene Summary	AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]

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