Elastase (CELA3B) (BC005216) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ELA3A; elastase 1; elastase 3B; elastase 3B, pancreatic; OTTHUMP00000002837; protease E
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC005216 edited GTCCTATCATCGCAAAACTCATGATGCTCCGGCTGCTCAGTTCCCTCCTCCTTGTGGCCG TTGCCTCAGGCTATGGCCCACCTTCCTCTCGCCCTTCCAGCCGCGTTGTCAATGGTGAGG ATGCGGTCCCCTACAGCTGGCCCTGGCAGGTTTCCCTGCAGTATGAGAAAAGTGGAAGCT TCTACCACACGTGTGGCGGTAGCCTCATCGCCCCCGACTGGGTTGTGACTGCCGGCCACT GCATCTCGAGCTCCTGGACCTACCAGGTGGTGTTGGGCGAGTACGACCGTGCTGTGAAGG AGGGCCCCGAGCAGGTGATCCCCATCAACTCTGGGGACCTCTTTGTGCATCCACTCTGGA ACCGCTCGTGTGTGGCCTGTGGCAATGACATCGCCCTCATCAAGCTCTCACGCAGCGCCC AGCTGGGAGACGCCGTCCAGCTCGCCTCACTCCCTCCCGCTGGTGACATCCTTCCCAACG AGACACCCTGCTACATCACCGGCTGGGGCCGTCTCTATACCAACGGGCCACTCCCAGACA AGCTGCAGGAGGCCCTGCTGCCCGTGGTGGACTATGAACACTGCTCCAGGTGGAACTGGT GGGGTTCCTCCGTGAAGAAGACCATGGTGTGTGCTGGAGGGGACATCCGCTCCGGCTGCA ACGGTGACTCTGGAGGACCCCTCAACTGCCCCACAGAGGATGGTGGCTGGCAGGTCCATG GCGTGACCAGCTTTGTTTCTGCCTTTGGCTGCAACACCCGCAGGAAGCCCACGGTGTTCA CTCGAGTCTCCGCCTTCATCGACTGGATTGAGGAGACCATAGCAAGCCACTAGAACCAAG GCCCAGCTGGCAGTGCTGATCGATCCCACATCCTGAATAAAGAATAAAGATCTCCCAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC005216 unedited NGTCAAAATTTGTATACGACTCATATAGGCGGACCGCGAATTCGCCATTACGGCCGGGGG TCCTATCATCGCAAAACTCATGATGCTCCGGCTGCTCAGTTCCCTCCTCCTTGTGGCCGT TGCCTCAGGCTATGGCCCACCTTCCTCTCGCCCTTCCAGCCGCGTTGTCAATGGTGAGGA TGCGGTCCCCTACAGCTGGCCCTGGCAGGTTTCCCTGCAGTATGAGAAAAGTGGAAGCTT CTACCACACGTGTGGCGGTAGCCTCATCGCCCCCGACTGGGTTGTGACTGCCGGCCACTG CATCTCGAGCTCCTGGACCTACCAGGTGGTGTTGGGCGAGTACGACCGTGCTGTGAAGGA GGGCCCCGAGCAGGTGATCCCCATCAACTCTGGGGACCTCTTTGTGCATCCACTCTGGAA CCGCTCGTGTGTGGCCTGTGGCAATGACATCGCCCTCATCAAGCTCTCACGCAGCGCCCA GCTGGGAGACGCCGTCCAGCTCGCCTCACTCCCTCCCGCTGGTGACATCCTTCCCAACGA GACACCCTGCTACATCACCGGCTGGGGCCGTCTCTATACCAACGGGCCACTCCCAGACAA GCTGCAGGAGGCCCTGCTGCCCGTGGTGGACTATGAACACTGCTCCAGGTGGAACTGGTG GGGTTCCTCCGTGAAGAGACCATGGTGTGTGCTGGANGGGACATCCGCTCCGGCTGCAAC GGTGACTCTGGANGACCCCCTCACTGCCCCACAGAGGATGGTGGCTGGCANGTCCATGGC GTGACCAGCTTTGTTTCTGCCTTTGGCTGCACACCCGCAGGAAGCCCACGGGTGTCACTC GAGTCTCCGCCTCATCGACTGGATTTGAGAGACATAGCNAGCCCTAGAACCAGGGCCAGC TGGCAGTGCTGATCGATCCCACATCCCTGATAAGAATAAAAA
Restriction Sites	Please inquire
ACCN	BC005216
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC005216.1, AAH05216.1
RefSeq Size	926 bp
Locus ID	23436
Protein Families	Druggable Genome, Protease
Gene Summary	Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq, May 2009]

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