UBE2Q1 (BD137288) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD137288, the custom clone sequence may differ by one or more nucleotides CGCCGGGGGCCGGGGGCGGCCCAGGGGGGGGCCCGGGGCCGGGGCCCTGCCTGAGGCGAGAGCTGAAGCT GCTCGAGTCCATCTTGCCACCGCGGCCACGAGCGCTTCCGCATTGCCAGCGCCTGCCTGGACGAGCTGAG CTGCGAGTTCCTGCTGGCTGGGGCCGGAGGGGCCGGGGCGGGGGCCGCGCCCGGACCGCATCTCCCCCCA CGGGGGTCGGTGCCTGGGGATCCTGTCCGCATCCACTGCAACATCACGGAGTCATACCCTGCTGTGCCCC CCATCTGGTCGGTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGGAGAGGCTGGTGGACATAAAGAA AGGGAATACTCTGCTATTGCAGCATCTGAAGAGGATCATCTCCGACCTGTGTAAACTCTATAACCTCCCT CAGCATCCAGATGTGGAGATGCTGGATCAACCCTTGCCAGCAGAGCAGTGCACACAGGAAGACGTGTCTT CAGAAGATGAAGATGAGGAGATGCCTGAGGACACAGAAGACTTAGATCACTATGAAATGAAAGAGGAAGA GCCAGCTGAGGGCAAGAAATCTGAAGATGATGGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAATT AAAAAGAACCAGAGGCAAGATTACTTAAATGGTGCAGTGTCTGGCTCGGTGCAGGCCACTGACCGGCTGA TGAAGGAGCTCAGGGATATATACCGATCACAGAGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAA TGACAGTCTGTATGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTGCACAACGATCTC CAGATCCTCAAAGAGAAAGAAGGAGCCGACTTCATTCTACTTAACTTTTCCTTTAAAGATAACTTTCCCT TTGACCCACCATTTGTCAGGGTTGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGCGGAGGGGCCAT CTGCATGGAACTTCTCACCAAACAGGGCTGGAGCAGTGCCTACTCCATAGAGTCAGTGATCATGCAGATC AGTGCCACACTGGTGAAGGGGAAAGCACGAGTGCAGTTTGGAGCCAACAAATCTCAATACAGTCTGACAA GAGCACAGCAGTCCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACACACCCCCAAAAGA AGACGGCTAACCCTGGAGTATCACCCTTCCTCCCTCCCCAGGCACCACTGGACCAATTACCTTTGAATGC TGTATTTGGATCTCACGCTGCCTCTGTGGTTCCCTCCCTCATTTTTCCTGGACGTGATAGCTCTGCCTAT TGCAGGACAATGATGGCTATTCTAAACGCTAAGGAAAAAAAACAAACACAGAACTGTTTCAAGTACTCAA GACTGACTTACAGACCAACCAACCACCTTGCTGGAACCCTTGCTAGCAGGCATTCTTATAAAAGAAACTT TCGAGCCTCCTTATATTGCTGGAAACTCAGCTGTGCTCCAGACTAGAGCCTCCTTACCTATGCTATGGAT TTTTAATTTATTTTCTCTTATTTCATGTACACTGCTTTTTTTGGTTACAGTGTATGATGGATGTGTATGA AAAAAATGTATCTTTGGGAAAACAATTACAGTTTGTTAATTTGGAAAAAAAAAAAATGACTCATCTTTAT TTTTATTCGCACATCCCACCCCATCCCCTGGAACTACTTGGGGAGGTGGGGAGGGGTACGAGTCCACTGT TGGCTTGGTAGGAGATGAGAGGCCGCATTTGCTGCTTAAGCAAGGGGAACTGGGGCTGAGCACACACCGG GGTGCCCCCGGATTTTTCCTCAAGGGCTCTGGAGCAACGGCACCGGCCCCTTGGGATGCA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD137288 unedited ATTTGTAATACGACCTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCTGTGCCCC CCATCTGGTCGGTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGGAGAGGCTGGTGG ACATAAAGAAAGGGAATACTCTGCTATTGCAGCATCTGAAGAGGATCATCTCCGACCTGT GTAAACTCTATAACCTCCCTCAGCATCCAGATGTGGAGATGCTGGATCAACCCTTGCCAG CAGAGCAGTGCACACAGGAAGACGTGTCTTCAGAAGATGAAGATGAGGAGATGCCTGAGG ACACAGAAGACTTAGATCACTATGAAATGAAAGAGGAAGAGCCAGCTGAGGGCAAGAAAT CTGAAGATGATGGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAATTAAAAAGAACC AGAGGCAAGATTACTTAAATGGTGCAGTGTCTGGCTCGGTGCAGGCCACTGACCGGCTGA TGAAGGAGCTCAGGGATATATACCGATCACAGAGTTTCAAAGGCGGAAACTATGCAGTCG AACTCGTGAATGACAGTCTGTATGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACA GCGCTTTGCACAACGATCTCCAGATCCTCANAGAGAAAGAAGGAGCCGACTTCATTCTAC TTTAACTTTTCTTTAAAGAAACTTTCCCTTTGACCCACCATTTGTCAGGGGTGTGTCTCC AGTCCTCTCTGGAGGGTATGTTCTGGGCGGAGGGGCCATCTGCATGGGACCTCTCACCAA CAGGGCTGGAGCAGTGCCTACTCCATAGAGTCAGTGATCATGCCGATCAGTGCCACACTG GTGAAAGGGGAAGCACGAATGCCANTTTGGNACCCACAAATCTCAATACAGTCTGACCAG AGCCAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BD137288 unedited GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAATTAACAAACTGTAATTGTTTTC CCAAAAAACATTTTTTTCATACACATCCATCATACACTGTAACCAAAAAAAGCAGTGTAC ATGAAATAAGAGAAAATAAATTAAAAATCCATAGCATAGGTAAGGAGGCTCTAGTCTGGA GCACAGCTGAGTTTCCAGCAATATAAGGAGGCTCGAAAGTTTCTTTTATAAAAATGCCTG CTAGCAAGGGTTCCAGCAAGGTGGTTGGTTGGTCTGTAAGTCAGTCTTGAGTACTTGAAA CAGTTCTGTGTTTGTTTTTTTTCCTTAGCGTTTAAAATAGCCATCATTGTCCTGCAATAG GCAGAGCTATCACGTCCAGGAAAAATGAGGGAGGGAACCACAGAGGCAGCGTGAGATCCA AATACAGCATTCAAAGGTAATTGGTCCAGTGGTGCCGTTTTTTTCGTGGATCTGCACCAA GGACTTGTAGGACTGCTGTGCTCTTGTCAGACTGTATTGAGATTTGTTGGCTCCAAACTG CACTCGTGCTTTCCCCTTCCCAGTGTGGCACTGATCTGCATGATCACTGACTCTATGGAG TAGGCACTGCTCCAGCCCTGTTTGGAGAGAAGTTCCATGCAGATGGCCTCTCCGCCAAGA CATACCCTCCAGAAGGACTGAAGACCCACCCTGACAATGGTGGCCAAAGGGAAAGCACTC TTAAAGAAAAGTCAGTAGATGAAGCCGCTCCTTCTTTCTCTTGAGGATCTGAGATCGTTT GCAAAGGCCTGCCTGCCACCTTGAAGAGTTGACTTTCATACTACAACGTCTTCACCATTC CACGCTATTTCCGCTCTGAACCTGGGACGATTATCCCGACTCTTATAACCGGAAGGGCTG CCCGACAAACTGACCTTAAGAAATCGCCCCGGGCTTTAATTTTCAGAACGCATTTCTTTC AGCCTATCCAG
Restriction Sites	NotI-NotI
ACCN	BD137288
Insert Size	1400 bp
OTI Disclaimer	The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required.
OTI Annotation	This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BD137288.1
RefSeq Size	1880 bp
RefSeq ORF	1880 bp
Locus ID	55585
Domains	UBCc
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is 98% identical to the mouse counterpart. [provided by RefSeq, Jul 2008]

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