MTGR1 (CBFA2T2) (NM_175864) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	core-binding factor, runt domain, alpha subunit 2; DKFZp313F2116; EHT; EHT, MTGR1, ZMYND3, DKFZp313F2116; ETO homolog on chromosome 20; MTG8-like protein; MTGR1; myeloid translocation gene-related protein 1; OTTHUMP00000030653; translocated to, 2; ZMYND3
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC127577 sequence for NM_175864 edited (data generated by NextGen Sequencing) ATGCCTGGATCGCCTGTGGAAGTGAAGATACAGTCCAGATCCTCACCTCCCACCATGCCA CCCCTCCCACCAATAAATCCTGGAGGACCGAGGCCAGTGTCCTTCACTCCTACTGCATTA AGCAATGGCATCAACCATTCTCCTCCTACCCTGAATGGTGCCCCATCACCGCCACAGAGA TTCAGCAATGGTCCTGCCTCCTCCACATCATCTGCACTCACAAATCAGCAATTGCCAGCC ACTTGTGGTGCTCGACAACTCAGCAAGTTGAAACGCTTTCTTACCACTCTGCAACAGTTT GGCAATGACATCTCCCCTGAGATTGGGGAGAAGGTGCGGACTCTTGTTCTTGCACTGGTG AACTCAACAGTGACAATTGAGGAATTCCACTGTAAGCTCCAAGAAGCCACAAACTTTCCC CTTCGTCCTTTTGTGATTCCATTTCTCAAGGCCAACCTGCCCCTGCTGCAGCGGGAACTG CTGCACTGCGCTCGGGCGGCCAAGCAGACCCCATCCCAGTACCTGGCTCAGCACGAACAC CTTCTGCTCAACACAAGCATTGCATCGCCTGCTGACTCGTCAGAGTTGCTCATGGAGGTG CACGGAAATGGGAAGAGGCCCAGTCCAGAGAGGAGAGAAGAGAATAGTTTTGATAGAGAC ACAATTGCTCCTGAGCCTCCTGCCAAGAGAGTATGTACCATCAGCCCTGCTCCTCGGCAC AGTCCTGCTCTCACTGTGCCCCTCATGAATCCCGGGGGCCAATTCCATCCTACCCCTCCA CCTCTTCAGCATTACACCTTAGAGGATATTGCAACTTCTCACCTGTATCGGGAACCCAAC AAGATGCTAGAGCATCGAGAAGTTCGTGATAGACACCACAGTCTTGGTCTAAATGGAGGC TATCAAGATGAGTTGGTAGATCATCGTTTGACAGAAAGGGAATGGGCTGATGAATGGAAA CATCTTGACCATGCGCTGAATTGCATTATGGAAATGGTAGAGAAAACAAGGCGCTCTATG GCAGTTCTGCGGCGCTGTCAGGAATCAGATCGTGAAGAACTCAACTACTGGAAAAGACGG TACAATGAAAACACAGAGCTGAGGAAAACGGGGACCGAGTTGGTCTCCAGGCAGCACAGC CCTGGGAGTGCAGATTCTCTCAGCAATGATTCTCAGAGAGAGTTCAACAGCAGGCCAGGT ACAGGATACGTACCTGTGGAGTTTTGGAAAAAAACAGAAGAAGCTGTGAATAAGGTGAAA ATTCAGGCCATGTCAGAAGTACAGAAGGCCGTCGCTGAGGCAGAGCAGAAAGCCTTTGAA GTGATTGCAACAGAGAGAGCACGAATGGAGCAAACCATAGCGGATGTCAAGCGGCAGGCC GCAGAGGATGCTTTCCTCGTCATCAATGAGCAAGAGGAGTCCACGGAGAACTGCTGGAAC TGTGGCCGCAAAGCCAGCGAGACATGCAGTGGCTGCAATATCGCGCGATACTGTGGCTCT TTCTGCCAGCACAAGGACTGGGAGCGGCACCACCGCCTCTGTGGTCAGAACCTGCATGGC CAGAGCCCCCACGGCCAGGGCCGGCCGCTGCTTCCTGTAGGCAGGGGCTCCTCTGCCAGG TCCGCCGACTGCAGCGTGCCCAGCCCAGCCCTCGACAAGACCTCGGCAACCACATCGCGT TCCTCAACACCTGCTTCTGTGACAGCTATCGACACCAACGGACTCTGA Clone variation with respect to NM_175864.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_175864 unedited TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCTGGTTAGCTCGG CGGCTGCAGATCTCGCGGCGACGCCTGCGAGGGACCCGGGCCGCGGGTCGAGGCGGGCGG CGCCTGCGAGGGACCCGTGTCGCGGGTAGAGGCGGGCGGCGCGCGGCGGCGGCGCTCGGC GATGGTAGGCGTCCCTGGAGCGGCCGCCTTCCAGCTTGGTCCTGAGAAAAGGGTGCCAGC GATGCCTGGATCGCCTGTGGAAGTGAAGATACAGTCCAGATCCTCACCTCCCACCATGCC ACCCCTCCCACCAATAAATCCTGGAGGACCGAGGCCAGTGTCCTTCACTCCTACTGCATT AAGCAATGGCATCAACCATTCTCCTCCTACCCTGAATGGTGCCCCATCACCGCCACAGAG ATTCAGCAATGGTCCTGCCTCCTCCACATCATCTGCACTCACAAATCAGCAATTGCCAGC CACTTGTGGTGCTCGACAACTCAGCAAGTTGAAACGCTTTCTTACCACTCTGCAACAGTT TGGCAATGACATCTCCCCTGAGATTGGGGAGAAGGTGCGGACTCTTGTTCTTGCACTGGT GAACTCAACAGTGACCATTGAGGAATTCCACTGGTAGCTCCAAGAAGCCACAAACTTTCC CCTTCGTCCTTTTGTGATTCCATTTCTCAAGGCCAACCTGCCCCTGCTGCAGCGGGAACT GCTGCACTGCGCTCGGGCGGNCCAGCAGACCCCATCCAGTACCTGGCTCAGCACGAACAC CTTTCTGCTCACACAAGCATTGCATCGCCTGCTGACTCGTCANAGNTGCTCATGGGAGTG CACGGNNATGGGGAGAGGCCCANTCCAGAGAGGAGAGAGAGAATAGTTTGATAGAGCACA TTTGCTCCTGAGCCTCTGGCCAGAGAGTATGACCATCAGCCTGCTCTCGGACAGTCTGTT TTCTGGT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_175864 unedited CGCGCCGCTTTCTAGTATCGAGTTTTTTTTTTTTTTTTTTGTGATGTGGGGGAAGTTTTA TTTGTTTTATGTTTTTGCTACTACGGGTGCTTTTATTGCAACAGTGACTTCTTTCCTGTG GACTCTGAGATCTTATCAAGTCTGAACAGCAGGGTTGAGAAGAGTCCTGAATGCCGCCTG TTGCTTACCCAAGTCCCCGTGAGGCCAATCAGTGTCATCTGCTGAGGATGGAGATGGGGC CGAAGGAGCTATTCAAGGGTGAGGCAGGCCGCTCCCTGACCGAAGGGCCTTCTACACAGC CTGCTGCCTGCCGCCTGCCGCCTCCAGCCTGGCTGAGCACATTGGACAGTCTGCTGACTC AAGGAGATGGGGAGGGGAGCTGGTGGTCTACACTGAAAAGGGGCCGGCTAAGTCAGCTTC AGTGGAGAGAGGAACAAACCAGGAAAGCCATGGAGGAGGCAGCTCAAGCTGGCTGCCCAT GTGCAGAGAATGGCACACTGGCTCCGCAGACAGCAAGTGCACAGAGAGGCTGCGGGGTGT TATTATTCAAGCTTGGACTCTTCCTGCCTCCAATTCATTTCTTGCCTGATGACCCGGCAG CTACATGCACGGGTTCCAACAGTCAGTCCCTCAGCCAAGCACCTCTGAGGTGCTGAGCAG CCATCATGGTTAGCAGAGTCCGTGGCTCATAGTCCCGTTGGTGTCGATAGCCTGTCACAG AACCAGTTGCTGAGGAACGCCATGTGGTTGCCGAGGTCTTGTCGAGGCCTTGGCTGGCAC GCCTGATTGGCGGACCTGTGAAAGGACCCCCTGCCTACAGAAGCAATAGCCGCCCCTGCC CGTGAGGCTTTGCCCTGCAGGTCTGACCACAGATCGGTGTGCCGCTCCAGTCTTGGCTGG AAAAAAGCACATTTGGCGAATTGGAGCCTGTTGTTTGCTGCTGGGCCCATTCCACATTTT CCGGACTCCTTGCCAT
Restriction Sites	NotI-NotI
ACCN	NM_175864
Insert Size	2440 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175864.1, NP_787060.1
RefSeq Size	6996 bp
RefSeq ORF	6996 bp
Locus ID	9139
Protein Families	Transcription Factors
Gene Summary	In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript but encodes the shorter isoform (MTGR1a).

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