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T (NM_003181) Human Untagged Clone
CAT＃: SC303281
T (untagged)-Human T, brachyury homolog (mouse) (T)
CNY 3,656.00
CNY 6,940.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SAVA; T; TFT |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_003181 edited
TCTCAGGTAGCGAGTCTGGGCTTCGGGGACGGCGGGGAGGGGAGCCGGACGGGAGGATGA GCTCCCCTGGCACCGAGAGCGCGGGAAAGAGCCTGCAGTACCGAGTGGACCACCTGCTGA GCGCCGTGGAGAATGAGCTGCAGGCGGGCAGCGAGAAGGGCGACCCCACAGAGCGCGAAC TGCGCGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGCTCACCAATGAGA TGATCGTGACCAAGAACGGCAGGAGGATGTTTCCGGTGCTGAAGGTGAACGTGTCTGGCC TGGACCCCAACGCCATGTACTCCTTCCTGCTGGACTTCGTGGCGGCGGACAACCACCGCT GGAAGTACGTGAACGGGGAATGGGTGCCGGGGGGCAAGCCGGAGCCGCAGGCGCCCAGCT GCGTCTACATCCACCCCGACTCGCCCAACTTCGGGGCCCACTGGATGAAGGCTCCCGTCT CCTTCAGCAAAGTCAAGCTCACCAACAAGCTCAACGGAGGGGGCCAGATCATGCTGAACT CCTTGCATAAGTATGAGCCTCGAATCCACATAGTGAGAGTTGGGGGTCCACAGCGCATGA TCACCAGCCACTGCTTCCCTGAGACCCAGTTCATAGCGGTGACTGCTTATCAGAACGAGG AGATCACAGCTCTTAAAATTAAGTACAATCCATTTGCAAAAGCTTTCCTTGATGCAAAGG AAAGAAGTGATCACAAAGAGATGATGGAGGAACCCGGAGACAGCCAGCAACCTGGGTACT CCCAATCAGGGGGGTGGCTTCTTCCTGGAACCAGCACCCTGTGTCCACCTGCAAATCCTC ATCCTCAGTTTGGAGGTGCCCTCTCCCTCCCCTCCACGCACAGCTGTGACAGGTACCCAA CCCTGAGGAGCCACCGGTCCTCACCCTACCCCAGCCCCTATGCTCATCGGAACAATTCTC CAACCTATTCTGACAACTCACCTGCATGTTTATCCATGCTGCAATCCCATGACAATTGGT CCAGCCTTGGAATGCCTGCCCATCCCAGCATGCTCCCCGTGAGCCACAATGCCAGCCCAC CTACCAGCTCCAGTCAGTACCCCAGCCTGTGGTCTGTGAGCAACGGCGCCGTCACCCCGG GCTCCCAGGCAGCAGCCGTGTCCAACGGGCTGGGGGCCCAGTTCTTCCGGGGCTCCCCCG CGCACTACACACCCCTCACCCATCCGGTCTCGGCGCCCTCTTCCTCGGGATCCCCACTGT ACGAAGGGGCGGCCGCGGCCACAGACATCGTGGACAGCCAGTACGACGCCGCAGCCCAAG GCCGCCTCATAGCCTCATGGACACCTGTGTCGCCACCTTCCATGTGAAGCAGCAAGGCCC AGGTCCCGAAAGATGCAGTGACTTTTTGTCGTGGCAGCCAGTGGTGACTGGATTGACCTA CTAGGTACCCAGTGGCAGTCTCAGGTTAAGAAGGAAATGCAGCCTCAGTAACTTCCTTTT CAAAGCAGTGGAGGAGCACAC >OriGene 5' read for NM_003181 unedited
GNNCGGCCGCGNAATTCGCCCTTGNAGCAGGNAGGTGGATTCTCAGTAGCGAGTCTGGGC TTCGGGGACGGCGGGGAGGGGAGCCGGACGGGAGGATGAGCTCCCCTGGCACCGAGAGCG CGGGAAAGAGCCTGCAGTACCGAGTGGACCACCTGCTGAGCGCCGTGGAGAATGAGCTGC AGGCGGGCAGCGAGAAGGGCGACCCCACAGAGCGCGAACTGCGCGTGGGCCTGGAGGAGA GCGAGCTGTGGCTGCGCTTCAAGGAGCTCACCAATGAGATGATCGTGACCAAGAACGGCA GGAGGATGTTTCCGGTGCTGAAGGTGAACGTGTCTGGCCTGGACCCCAACGCCATGTACT CCTTCCTGCTGGACTTCGTGGCGGCGGACAACCACCGCTGGAAGTACGTGAACGGNGAAT GGGTGCCGGGGGGCAAGCCGGAGCCGCAGGCGCCCAGCTGCGTCTACATCCACCCCGACT CGCCCAACTTCGGGGCCCACTGGATGAAGGCTCCCGTCTCCTTCAGCANAGTCAAGCTCA CCAACNAGCTCAACGGGAGGNGGCCAGATCATGCTGAACTCCTTTGCATAGTATGAGCCT CGAATCCACATAGTGAGAGTTGGGGGGTCCACAGCGCATGATCACCAGCCACTGCTTCCC TGAGACCCAGTTCATAGCGGTGACTGCTTATCAGAACGAGGAGATCACAGCTCTTAAAAT TAAGTANCATCCATTTGCAAAAGCTTTCCTTGATGCANAGGANAGAAGTGATCACAAAGA GATGAATGGANGACCCGNAGACAGCCAGCAACCTGGGTACTCCCATCA >Reverse primer walk for NM_003181 unedited
GACTTCTGGATAGTCAATCCATTCCCACTGGCTGCCACGAAAAAATCACTGCATCTTTCG GGACCTGGGCCTTGCTGCTTCACATGGAAGGTGGCGACACAGGTGTCCATGAGGCTATGA GGCGGCCTTGGGCTGCGGCGTCGTACTGGCTGTCCACGATGTCTGTGGCCGCGGCCGCCC CTTCGTACAGTGGGGATCCCGAGGAAGAGGGCGCCGAGACCGGATGGGTGAGGGGTGTGT AGTGCGCGGGGGAGCCCCGGAAGAACTGGGCCCCCAGCCCGTTGGACACGGCTGCTGCCT GGGAGCCCGGGGTGACGGCGCCGTTGCTCACAGACCACAGGCTGGGGTACTGACTGGAGC TGGTAGGTGGGCTGGCATTGTGGCTCACGGGGAGCATGCTGGGATGGGCAGGCATTCCAA GGCTGGACCAATTGTCATGGGATTGCAGCATGGATAAACATGCAGGTGAGTTGTCAGAAT AGGTTGGAGAATTGTTCCGATGAGCATAGGGGCTGGGGTAGGGTGAGGACCGGTGGCTCC TCAGGGTTGGGTACCTGTCACAGCTGTGCGTGGAGGGGAGGGAGAGGGCACCTCCAAACT GAGGATGAGGATTTGCAGGTGGACACAGGGTGCTGGTTCCAGGAAGAAGCCACCCCCCTG ATTGGGAGTACCCAGGTTGCTGGCTGTCTCCGGGTTCCTCCATCATCTCTTTGTGATCAC TTCTTTCCTTTGCATCAAGGAAAGCTTTTGCAAATGGATTGTACTTAATTTTAAGAGCTG TGATCTCCTCGTTCTGATAAGCAGTCACCGCTATGAACTGGGTCTCAGGGAAGCAGTGGC TA |
| Restriction Sites | Please inquire |
| ACCN | NM_003181 |
| Insert Size | 1500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | There is 4 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the insertion of 1aa and substituion of 1 aa. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003181.2, NP_003172.1 |
| RefSeq Size | 2518 bp |
| RefSeq ORF | 1308 bp |
| Locus ID | 6862 |
| UniProt ID | O15178 |
| Protein Families | ES Cell Differentiation/IPS, Transcription Factors |
| Gene Summary | The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. [provided by RefSeq, Sep 2018] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nonviral Transfection With Brachyury Reprograms Human Intervertebral Disc Cells to a Pro-Anabolic Anti-Catabolic/Inflammatory Phenotype: A Proof of Concept Study
,Tang, S;Richards, J;Khan, S;Hoyland, J;Gallego-Perez, D;Higuita-Castro, N;Walter, B;Purmessur, D;,
J. Orthop. Res.
,PubMed ID 31286562
[TBXT]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218530 | T (Myc-DDK-tagged)-Human T, brachyury homolog (mouse) (T) |
CNY 3,656.00 |
|
| RC218530L1 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC218530L2 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), mGFP tagged |
CNY 5,890.00 |
|
| RC218530L3 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC218530L4 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), mGFP tagged |
CNY 6,056.00 |
|
| RG218530 | T (tGFP-tagged) - Human T, brachyury homolog (mouse) (T) |
CNY 5,256.00 |
