TH1L (NELFCD) (NM_198976) Human Untagged Clone

CAT#: SC307839

NELFCD (untagged)-Human TH1-like (Drosophila) (TH1L), transcript variant 1



  "NM_198976" in other vectors (4)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
NELFCD Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HSPC130; NELF-C; NELF-D; TH1; TH1L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC307839 representing NM_198976.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCGCCGCGCTCGCTCGCGGGAGGGCATGGCGGGGGCCGTGCCGGGCGCCATCATGGACGAGGACTAC
TACGGGAGCGCGGCCGAGTGGGGCGACGAGGCTGACGGCGGCCAGCAGGAGGATGATTCTGGAGAAGGA
GAGGATGATGCGGAGGTTCAGCAAGAATGCCTGCATAAATTTTCCACCCGGGATTATATCATGGAACCC
TCCATCTTCAACACTCTGAAGAGGTATTTTCAGGCAGGAGGGTCTCCAGAGAATGTTATCCAGCTCTTA
TCTGAAAACTACACCGCTGTGGCCCAGACTGTGAACCTGCTGGCCGAGTGGCTCATTCAGACAGGTGTT
GAGCCAGTGCAGGTTCAGGAAACTGTGGAAAATCACTTGAAGAGTTTGCTGATCAAACATTTTGACCCC
CGCAAAGCAGATTCTATTTTTACTGAAGAAGGAGAGACCCCAGCGTGGCTGGAACAGATGATTGCACAT
ACCACGTGGCGGGACCTTTTTTATAAACTGGCTGAAGCCCATCCAGACTGTTTGATGCTGAACTTCACC
GTTAAGCTTATTTCTGACGCAGGGTACCAGGGGGAGATCACCAGTGTGTCCACAGCATGCCAGCAGCTA
GAAGTGTTCTCGAGAGTGCTCCGGACCTCTCTAGCTACAATTTTAGATGGAGGAGAAGAAAACCTTGAA
AAAAATCTCCCTGAGTTTGCCAAGATGGTGTGCCACGGGGAGCACACGTACCTGTTTGCCCAGGCCATG
ATGTCCGTGCTGGCCCAGGAGGAGCAGGGGGGCTCCGCTGTGCGCAGGATCGCCCAGGAAGTGCAGCGC
TTTGCCCAGGAGAAAGGTCATGACGCCAGTCAGATCACACTAGCCTTGGGCACAGCTGCCTCCTACCCC
AGGGCCTGCCAGGCTCTCGGGGCCATGCTGTCCAAAGGAGCCCTGAACCCTGCTGACATCACCGTCCTG
TTCAAGATGTTCACAAGCATGGACCCTCCTCCGGTTGAACTTATCCGCGTTCCAGCCTTCCTGGACCTG
TTCATGCAGTCACTCTTTAAACCAGGGGCTCGGATCAACCAGGACCACAAGCACAAATACATCCACATC
TTGGCGTACGCAGCAAGCGTGGTTGAGACCTGGAAGAAGAACAAGCGAGTGAGCATCAATAAAGATGAG
CTGAAGTCAACGTCAAAAGCTGTCGAAACCGTTCACAATTTGTGTTGCAACGAGAACAAAGGGGCCTCT
GAACTAGTGGCAGAATTGAGCACACTTTATCAGTGTATTAGGTTTCCAGTGGTAGCAATGGGTGTGCTG
AAGTGGGTGGATTGGACTGTATCAGAACCAAGGTACTTTCAGCTGCAGACTGACCATACCCCTGTCCAC
CTGGCGTTGCTGGATGAGATCAGCACCTGCCACCAGCTCCTGCACCCCCAGGTCCTGCAGCTGCTTGTT
AAGCTTTTTGAGACTGAGCACTCCCAGCTGGACGTGATGGAGCAGCTTGAGTTGAAGAAGACACTGCTG
GACAGGATGGTTCACCTGCTGAGTCGAGGTTATGTACTTCCTGTTGTCAGTTACATCCGAAAGTGTCTG
GAGAAGCTGGACACTGACATTTCACTCATTCGCTATTTTGTCACTGAGGTGCTGGACGTCATTGCTCCT
CCTTATACCTCTGACTTCGTGCAACTTTTCCTCCCCATCCTGGAGAATGACAGCATCGCAGGTACCATC
AAAACGGAAGGCGAGCATGACCCTGTGACGGAGTTTATAGCTCACTGCAAATCTAACTTCATCATGGTG
AACTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_198976
Insert Size 1800 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_198976.2
RefSeq Size 2320 bp
RefSeq ORF 1800 bp
Locus ID 51497
UniProt ID Q8IXH7
Protein Families Transcription Factors
MW 67.3 kDa
Gene Summary The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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