ARHGAP21 (NM_020824) Human Untagged Clone

CAT#: SC309106

ARHGAP21 (untagged)-Human Rho GTPase activating protein 21 (ARHGAP21)



  "NM_020824" in other vectors (3)

CNY 37,810.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ARHGAP10
Vector PCMV6-Neo
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_020824 edited
GCGGGATCGTCCGGCTGCGGGGTCCGGCTCACGCCGGGCGGGCGGGCGGGGCGCGCTGTC
CCGGAGCCGGCCAGCCCCGTGCGGATCCACTCGTCCCGCTTCCTCTGGATCTCTCTAGGA
GCTGACACTCGAACCTTCACGACCCATTCGGATTTTTCCAGGACTCAGGAGTGGCACTGG
GAAGAAGGGGACCGCTTTCTGCAATTGGCCTCGACACTGGCTGCCAAGAAGACCTGTCGC
CTTTGTTTTTAAGTCTCCAGAAATGGAAGAAGAAGGCGAAGTCAGTTGAAGTCACGAGAA
ATCAGCGAGGCATTTGAAGGCGCTTCCTGAAAACCTTTATTCCCTGGCACGTTGTCTGTT
TCAACAGCCCTGCCCCTCCTCGGAGCCTGCTTGTGGAATTCTTCCCCTTCGGGTGTGTGG
TGGCATTCCCCGCCACGTCCAATGTGGACTCCAAAGGATTTGTCCCTTCTTTGTCATTTG
AAATGAAATGATGGCCACGCGTCGGACTGGTCTGTCTGAGGGAGATGGTGACAAGCTCAA
GGCCTGCGAGGTCTCAAAAAATAAAGATGGAAAAGAACAAAGTGAAACTGTATCACTGTC
TGAAGATGAAACATTCTCCTGGCCAGGTCCCAAAACAGTTACGTTGAAAAGAACATCTCA
AGGCTTTGGTTTTACATTAAGACATTTTATTGTTTATCCCCCAGAGTCTGCAATTCAATT
TTCATATAAGGATGAAGAAAATGGAAACAGAGGAGGAAAACAAAGAAACCGCTTGGAACC
AATGGATACCATATTTGTTAAGCAAGTTAAAGAAGGAGGACCTGCTTTTGAAGCTGGATT
ATGTACAGGTGACCGAATTATAAAAGTCAATGGAGAAAGTGTTATTGGCAAAACCTATTC
CCAAGTAATTGCTTTAATTCAAAACAGTGATACAACATTGGAACTTAGTGTTATGCCAAA
AGATGAAGACATTCTCCAAGTGCTACAGTTTACAAAGGATGTCACAGCACTGGCATATTC
TCAAGATGCCTACCTGAAAGGCAACGAAGCTTATAGCGGCAATGCCCGCAATATACCTGA
ACCTCCACCAATCTGCTATCCCTGGCTGCCATCTGCCCCATCAGCCATGGCACAGCCAGT
TGAAATATCTCCTCCTGACTCATCATTGAGCAAACAGCAAACCAGTACACCAGTACTGAC
ACAACCTGGTAGGGCCTATAGAATGGAAATACAAGTGCCTCCATCACCAACAGATGTTGC
AAAATCAAACACAGCAGTGTGTGTTTGCAATGAAAGTGTAAGGACTGTCATTGTGCCTTC
TGAGAAGGTTGTAGATTTGTTATCCAATAGAAACAACCATACAGGTCCTTCACATAGAAC
TGAAGAAGTGAGGTATGGCGTGAGTGAGCAGACCTCTTTAAAAACAGTGTCAAGAACCAC
ATCACCACCATTATCAATTCCCACCACTCATCTAATTCATCAGCCTGCAGGCTCCAGATC
ACTGGAACCTTCTGGAATTTTACTTAAGTCTGGAAATTACAGTGGACATTCTGATGGAAT
CTCAAGCAGCAGATCTCAAGCTGTGGAGGCTCCCTCTGTATCTGTTAATCACTATTCGCC
AAATTCCCATCAGCACATAGACTGGAAAAACTATAAAACTTACAAAGAGTATATTGATAA
CAGACGATTGCACATAGGTTGTCGGACAATACAAGAAAGATTAGATAGTTTAAGAGCAGC
ATCTCAAAGCACGACAGATTATAACCAGGTCGTCCCCAACCGCACTACTTTGCAGGGACG
ACGTCGAAGCACCTCTCATGATCGAGTGCCCCAGTCTGTCCAGATACGGCAACGCAGTGT
GTCCCAAGAAAGACTGGAAGATTCTGTGCTAATGAAGTATTGTCCAAGAAGTGCATCTCA
AGGAGCACTGACGTCTCCATCTGTTAGTTTTAGTAATCATAGAACTCGTTCATGGGATTA
TATTGAGGGACAGGATGAAACCTTAGAAAATGTCAATTCTGGAACTCCAATACCTGATTC
CAATGGAGAGAAAAAACAGACTTACAAGTGGAGTGGGTTTACTGAACAGGATGATAGACG
AGGTATTTGTGAAAGACCTAGGCAGCAAGAAATTCATAAATCTTTTCGAGGTTCCAATTT
TACTGTGGCTCCAAGCGTTGTTAATTCTGATAACAGGCGAATGAGTGGTAGAGGAGTGGG
ATCTGTGTCGCAGTTTAAAAAAATTCCACCAGATCTAAAAACATTGCAGTCAAACAGAAA
TTTTCAGACTACTTGTGGAATGTCACTGCCTCGGGGTATTTCACAAGACAGGTCACCTCT
TGTGAAAGTCCGAAGTAATTCTCTGAAAGCTCCTTCCACGCATGTCACAAAACCATCATT
TAGCCAGAAATCATTTGTTTCTATCAAAGACCAAAGACCAGTAAATCACTTGCATCAGAA
CAGTCTGTTGAATCAGCAGACATGGGTAAGGACTGACAGTGCCCCCGATCAGCAAGTGGA
GACTGGGAAATCCCCCTCTTTATCTGGAGCCTCTGCCAAGCCTGCCCCTCAGTCGAGTGA
AAACGCTGGTACTTCAGATTTAGAACTACCTGTCAGTCAAAGGAATCAAGATTTAAGTTT
ACAAGAGGCTGAAACTGAGCAATCAGATACTTTAGATAATAAAGAAGCTGTCATCCTAAG
GGAAAAACCTCCATCTGGACGCCAGACACCGCAGCCTTTAAGGCATCAGTCTTACATCTT
GGCAGTAAATGACCAGGAGACCGGGTCAGACACTACCTGCTGGCTGCCCAATGATGCACG
TCGAGAGGTCCACATAAAAAGAATGGAGGAAAGAAAAGCCTCGAGTACCAGTCCGCCTGG
CGATTCTTTGGCTTCCATCCCATTTATAGATGAACCAACTAGCCCTAGCATTGATCATGA
TATTGCACATATCCCTGCCTCTGCTGTTATATCAGCCTCTACCTCTCAGGTCCCCTCCAT
AGCAACAGTTCCTCCTTGCCTCACAACTTCAGCTCCATTAATTCGCCGTCAGCTCTCACA
TGACCACGAATCTGTTGGCCCTCCTAGCCTGGATGCTCAGCCCAACTCAAAGACAGAAAG
ATCAAAATCATATGATGAGGGTCTGGATGATTACAGAGAAGATGCAAAATTGTCCTTTAA
GCACGTATCTAGTCTGAAGGGAATCAAGATCGCAGACAGCCAAAAGTCATCAGAAGACTC
TGGGTCCAGAAAAGATTCTTCCTCAGAGGTCTTCAGTGATGCTGCCAAGGAAGGGTGGCT
TCATTTCCGACCCCTTGTCACCGATAAGGGCAAGCGAGTTGGTGGAAGTATTCGGCCATG
GAAACAGATGTATGTTGTCCTTCGGGGTCATTCACTTTACCTGTACAAAGATAAAAGAGA
GCAGACGACTCCGTCTGAGGAAGAGCAGCCCATCAGTGTTAATGCTTGCTTGATAGACAT
CTCTTACAGTGAGACCAAGAGGAAAAATGTGTTTCGACTCACCACGTCCGACTGTGAATG
CCTGTTTCAGGCTGAAGACAGAGATGATATGCTAGCTTGGATCAAGACGATCCAGGAGAG
CAGCAACCTAAACGAAGAGGACACTGGAGTCACTAACAGGGATCTAATTAGTCGAAGAAT
AAAAGAATACAACAATCTGATGAGCAAAGCAGAACAGTTGCCAAAAACACCTCGCCAGAG
TCTCAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACA
CTCTCCGAAGGAAGAGTCGGAAAGGAAACTTCTCAGTAAAGATGATACCAGTCCCCCAAA
AGACAAAGGCACATGGAGAAAAGGCATTCCAAGTATCATGAGAAAGACATTTGAGAAAAA
GCCAACTGCTACAGGAACTTTCGGCGTCCGACTAGATGACTGCCCACCAGCTCATACTAA
TCGGTATATTCCATTAATAGTTGACATATGTTGCAAATTAGTTGAAGAAAGAGGTCTTGA
ATATACAGGTATTTATAGAGTTCCTGGAAATAATGCAGCCATCTCAAGTATGCAAGAAGA
ACTCAACAAGGGAATGGCTGATATTGATATACAAGATGATAAATGGCGAGATTTGAATGT
GATAAGCAGTTTACTAAAATCCTTCTTCAGAAAACTCCCTGAGCCTCTCTTCACAAATGA
TAAATATGCTGATTTTATTGAAGCCAATCGTAAAGAAGATCCTCTAGATCGTCTGAAAAC
ATTAAAAAGACTAATTCACGATTTGCCTGAACATCATTATGAAACACTTAAGTTCCTTTC
AGCTCATCTGAAGACAGTGGCAGAAAATTCAGAAAAAAATAAGATGGAACCAAGAAACCT
AGCAATAGTGTTTGGTCCCACCCTTGTTCGAACATCAGAAGACAACATGACCCACATGGT
CACCCACATGCCTGACCAGTACAAGATTGTAGAAACGCTCATCCAGCACCATGACTGGTT
TTTCACAGAAGAAGGTGCTGAAGAGCCTCTTACAACAGTGCAGGAGGAAAGCACAGTAGA
CTCCCAGCCAGTGCCAAACATAGATCATTTACTCACCAACATTGGAAGGACAGGAGTCTC
CCCAGGAGATGTATCAGATTCAGCTACTAGTGACTCAACAAAATCTAAGGGTTCTTGGGG
ATCTGGAAAGGATCAGTATAGCAGGGAACTGCTTGTGTCCTCCATCTTTGCAGCTGCTAG
TCGCAAGAGGAAGAAGCCGAAAGAAAAAGCACAGCCTAGCAGCTCAGAAGATGAACTGGA
CAATGTATTTTTTAAGAAAGAAAATGTGGAACAGTGTCACAATGATACTAAAGAGGAGTC
CAAAAAAGAAAGTGAGACACTGGGCAGAAAACAGAAGATCATCATTGCCAAAGAAAACAG
CACTAGGAAAGACCCCAGCACGACAAAAGATGAAAAGATATCACTAGGAAAAGAGAGCAC
GCCTTCTGAAGAACCCTCACCACCACACAACTCAAAACACAACAAGTCACCAACTCTCAG
CTGTCGCTTTGCCATCCTGAAAGAGAGCCCCAGGTCACTTCTGGCACAGAAGTCCTCCCA
CCTTGAAGAGACAGGCTCTGACTCTGGCACTTTGCTCAGCACGTCTTCCCAGGCCTCCCT
GGCAAGGTTTTCCATGAAGAAATCAACCAGTCCAGAAACGAAACATAGCGAGTTTTTGGC
CAACGTCAGCACCATCACCTCAGATTATTCCACCACATCGTCTGCTACATACTTGACTAG
CCTGGACTCCAGTCGACTGAGCCCTGAGGTGCAATCCGTGGCAGAGAGCAAGGGGGACGA
GGCAGATGACGAGAGAAGCGAACTCATCAGTGAAGGGCGGCCTGTGGAAACCGACAGCGA
GAGCGAGTTTCCCGTGTTCCCCACAGCCTTGACTTCAGAGAGGCTTTTCCGAGGAAAACT
GCAAGAAGTGACTAAGAGCAGCCGGAGAAATTCTGAAGGAAGTGAATTAAGTTGCACCGA
GGGAAGTTTAACATCAAGTTTAGATAGCCGGAGACAGCTCTTCAGTTCCCATAAACTCAT
CGAATGTGATACTCTTTCCAGGAAAAAATCAGCTAGATTCAAGTCAGATAGTGGAAGTCT
AGGAGATGCCAAGAATGAGAAAGAAGCACCTTCGTTAACTAAAGTGTTTGATGTTATGAA
AAAAGGAAAGTCAACTGGGAGTTTACTGACACCCACCAGAGGCGAATCCGAAAAACAGGA
ACCCACATGGAAAACGAAAATAGCAGATCGGTTAAAACTGAGACCCAGAGCCCCTGCGGA
TGACATGTTTGGAGTAGGGAATCACAAAGTGAATGCCGAGACTGCTAAAAGGAAAAGCAT
CCGGCGCAGACATACACTAGGAGGGCACAGAGATGCTACCGAAATCAGCGTTTTGAATTT
TTGGAAAGTGCATGAGCAGAGCGGGGAGAGAGAATCTGAACTTTCAGCTGTAAACCGGTT
AAAACCAAAATGCTCAGCCCAGGACCTTTCCATCTCAGACTGGCTGGCCAGGGAACGCCT
ACGCACCAGTACCTCTGACCTTAGCAGAGGAGAAATCGGAGATCCCCAGACAGAGAACCC
AAGCACACGAGAAATAGCCACGACCGACACACCTTTGTCTCTTCATTGCAACACAGGCAG
TTCTTCCAGCACCTTGGCTTCAACAAACAGGCCCCTTCTTTCCATACCACCACAGTCACC
TGACCAAATAAACGGAGAAAGCTTCCAGAACGTGAGCAAAAATGCTAGTTCTGCAGCGAA
TGCCCAACCTCATAAACTGTCTGAAACCCCAGGCAGTAAAGCAGAGTTTCATCCCTGTCT
TTAAACTGGGGGTATGTCCACTCTAGCAAGTAAAAAAACTACTGTTACACGTTCCAGTAA
CTCTGTCAATATTTTCTTGTATCAGAATTGTTATTATGCAGCCTTCATTTGGGCTGGTTT
CATCATTTTGCACTGTGAAATAGCTTTACAGTGCATTACTACAGCCAGAAGAACATATAT
ATATATATATATTTAAAAATATATCGGATAGTTGTATACAAATGAGCAAGGTATTTGTTG
CAACTTACTACATAGCATATACCCAAAATCACTGAAGAAAATCGCTGGCATCAGTGTGCA
GCAAATTTGTTCTTTTGGTTTCATCACTAACAAAAGT
Restriction Sites Please inquire     
ACCN NM_020824
Insert Size 7100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to be perfect to match to NM_020824.3.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020824.2, NP_065875.2
RefSeq Size 7185 bp
RefSeq ORF 5874 bp
Locus ID 57584
UniProt ID Q5T5U3
Domains RhoGAP, PDZ, PH
Gene Summary ARHGAP21 functions preferentially as a GTPase-activating protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3 complex (MIM 604221) and F-actin dynamics at the Golgi through control of CDC42 activity (Dubois et al., 2005 [PubMed 15793564]).[supplied by OMIM, Mar 2008]
Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: The 5'-most in-frame translation start codon is selected for this RefSeq and is well-conserved among mammalian species. An alternative start codon that would reduce the protein length by 1 aa is also present. The use of the downstream start codon is assumed in the literature, including PMIDs:12056806, 15793564 and 17347647.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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