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Prune homolog 2 (PRUNE2) (NM_015225) Human Untagged Clone
CAT＃: SC318745
PRUNE2 (untagged)-Human prune homolog 2 (Drosophila) (PRUNE2)
CNY 59,470.00
货期*
12周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BMCC1; BNIPXL; C9orf65; KIAA0367 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_015225, the custom clone sequence may differ by one or more nucleotides
ATGGAAGAATTTTTGCAACGCGCCAAATCTAAACTGAATCGAAGCAAACGCTTGGAGAAG GTCCATGTGGTTATTGGGCCTAAATCGTGTGACTTGGATTCTCTCATTTCTACCTTCACA TATGCTTACTTTCTAGACAAGGTCAGTCCACCAGGGGTTCTGTGTTTACCAGTGCTGAAC ATACCAAGAACTGAATTCAACTACTTCACCGAGACGAGGTTTATTTTAGAAGAGCTAAAT ATTTCCGAATCATTCCACATATTCCGGGATGAAATTAACCTGCATCAGCTAAATGATGAA GGGAAGTTATCGATAACACTTGTTGGCAGCAGTGTGCTGGCGAGTGAAGACAAAACTTTA GAATCAGCAGTTGTCAAAGTCATTAATCCGGTTGAGCAGAGCGATGCCAACGTTGAGTTC CGAGAGTCTTCCTCTTCTCTCGTGCTAAAGGAGATTCTCCAAGAGGCTCCTGAGCTCATC ACCGAGCAACTGGCTCATCGCCTCAGAGGTAGCATTCTTTTCAAGTGGATGACCATGGAA TCAGAGAAGATCTCAGAGAAGCAGGAGGAAATTCTTTCTATCCTGGAAGAAAAATTTCCT AACTTGCCTCCAAGAGAGGACATCATCAACGTCCTACAGGAGACCCAGTTCAGTGCTCAG GGTTTAAGTATTGAACAGACAATGTTGAAAGATCTAAAGGAGCTGTCAGATGGAGAAATA AAAGTGGCCATTAGTACTGTGAGCATGAACCTTGAGAATTGTCTATTTCACAGCAATATT ACCAGTGACTTGAAAGCATTTACAGACAAGTTTGGTTTTGATGTCCTCATCCTGTTCTCC AGCTATCTGTCAGAGGAGCAGCAGCCGAGACGACAGATTGCTGTGTACTCAGAAAACATG GAGCTGTGCAGTCAGATTTGCTGTGAGCTGGAAGAGTGTCAGAACCCTTGCCTAGAACTG GAGCCCTTTGACTGTGGCTGTGATGAGATCCTGGTGTACCAACAAGAGGACCCTTCAGTG ACTTGTGATCAGGTGGTTCTCGTTGTCAAGGAAGTCATCAACAGGAGGTGTCCAGAGATG GTCTCCAATAGCCGGACATCCTCAACAGAAGCCGTGGCAGGCAGTGCCCCCCTCTCCCAG GGGTCTTCTGGGATTATGGAATTGTATGGTTCTGACATAGAGCCACAACCCAGCTCTGTG AATTTCATAGAGAACCCTCCAGATCTCAATGATTCTAACCAGGCTCAGGTGGATGCCAAT GTAGACCTTGTTAGCCCAGACAGCGGACTGGCTACCATTAGGAGCAGCCGCTCATCCAAG GAGAGCTCTGTTTTCCTCAGTGACGACAGCCCCGTGGGAGAAGGTGCTGGGCCTCACCAC ACCCTTCTCCCAGGGCTTGACTCCTACAGCCCCATCCCTGAAGGGGCGGTGGCGGAGGAA CATGCATGGTCTGGAGAACACGGTGAGCACTTCGACCTCTTCAATTTTGACCCAGCACCC ATGGCTTCTGGGCAGTCCCAGCAATCTTCTCATTCTGCAGACTACTCCCCAGCAGATGAC TTCTTCCCCAACAGTGACCTGTCAGAAGGACAGCTCCCCGCTGGGCCTGAAGGACTTGAT GGCATGGGAACCAACATGTCTAATTATTCATCCAGTTCACTTTTGTCAGGGGCTGGCAAA GATAGCCTTGTGGAACATGATGAGGAGTTTGTCCAGAGACAAGACAGTCCCAGAGATAAC TCTGAAAGAAATTTGAGCCTGACAGATTTTGTGGGAGATGAATCCCCTTCCCCAGAAAGG CTAAAAAATACTGGAAAGAGGATCCCACCAACACCCATGAATAGTTTAGTAGAAAGCTCG CCATCCACTGAAGAACCAGCCTCACTCTATACAGAAGATATGACCCAAAAAGCAACTGAC ACAGGTCACATGGGGCCACCTCAGACCCATGCACGGTGCAGCAGCTGGTGGGGTGGTTTG GAAATTGACTCCAAAAATATTGCAGATGCGTGGAGTTCCAGTGAACAGGAATCTGTTTTC CAGAGCCCTGAATCATGGAAAGAGCATAAGCCAAGCTCCATTGATAGGAGAGCCTCAGAT TCTGTATTTCAACCAAAGAGCCTCGAATTTACAAAGTCAGGTCCCTGGGAGTCTGAATTT GGTCAGCCTGAACTGGGTAGCAATGATATTCAAGACAAAAATGAGGAAAGCTTGCCGTTC CAGAACCTGCCCATGGAGAAGTCACCTTTGCCAAATACATCTCCCCAAGGAACAAACCAC CTGATAGAAGACTTTGCTTCTTTGTGGCATTCTGGTCGCTCTCCCACAGCCATGCCCGAG CCCTGGGGAAATCCTACAGATGATGGTGAACCAGCAGCTGTGGCGCCATTCCCAGCCTGG AGTGCATTTGGTAAAGAAGATCATGATGAAGCTTTAAAAAATACCTGGAATTTGCACCCA ACAAGCAGCAAGACACCTTCTGTTAGGGACCCGAATGAGTGGGCCATGGCAAAAAGTGGG TTTGCCTTTTCTTCTTCAGAACTACTGGACAATTCACCCAGTGAGATAAACAATGAAGCA GCTCCAGAAATCTGGGGCAAGAAAAACAATGACTCCAGGGATCACATCTTTGCACCTGGA AATCCCAGTTCTGATCTGGATCACACATGGACTAATTCTAAGCCACCAAAAGAAGATCAG AATGGTTTAGTGGATCCTAAAACTAGGGGCAAGGTATATGAAAAGGTAGATTCCTGGAAC CTTTTTGAGGAGAATATGAAGAAAGGAGGGTCAGATGTCCTAGTTCCTTGGGAAGATTCC TTCTTATCTTACAAATGTTCTGATTACAGTGCATCCAACCTAGGAGAAGATTCGGTGCCT TCCCCCTTAGATACCAATTATTCCACCTCAGACTCTTACACATCACCAACATTTGCTGGA GACGAAAAGGAAACTGAACACAAGCCATTTGCTAAAGAGGAAGGTTTTGAGTCAAAAGAT GGTAACTCCACGGCAGAGGAGACTGACATTCCTCCTCAGTCACTGCAACAGTCATCTCGA AATCGAATCAGTTCAGGTCCTGGGAACCTAGACATGTGGGCTTCACCTCATACAGATAAC AGTTCTGAAATAAATACCACTCACAACCTGGATGAAAATGAACTCAAGACAGAGCACACA GATGGTAAGAATATCTCCATGGAGGATGACGTCGGGGAAAGCAGCCAGTCCAGTTACGAC GACCCCAGCATGATGCAACTGTACAATGAAACAAACCGACAACTCACACTTTTGCACAGC AGCACCAACTCCCGGCAGACGGCCCCTGACAGTCTCGACTTGTGGAACAGAGTGATTTTG GAGGATACTCAGTCCACTGCAACGATCTCAGATATGGACAATGATTTGGACTGGGATGAC TGCAGTGGGGGTGCGGCAATCCCCAGTGATGGTCAAACAGAAGGATACATGGCTGAAGGT TCCGAGCCGGAAACCCGATTTACAGTGAGACAGCTGGAACCCTGGGGCTTGGAGTATCAG GAAGCAAATCAGGTAGATTGGGAGCTCCCTGCCTCTGATGAGCATACCAAGGACAGTGCT CCCAGTGAACATCACACATTGAATGAGAAAAGTGGGCAGCTAATTGCAAACAGTATTTGG GATTCTGTCATGAGAGATAAAGACATGTCATCATTCATGTTACCAGGCTCCTCACATATC ACAGATTCAGAGCAAAGGGAATTGCCTCCTGAAATCCCCAGCCATTCAGCAAATGTTAAA GACACTCATTCCCCAGATGCGCCAGCAGCCTCTGGAACCAGTGAATCAGAGGCACTTATA TCTCATCTTGACAAGCAGGACACAGAGAGGGAAACCCTGCAAAGTGATGCAGCATCCTTG GCGACTAGGCTTGAGAATCCAGGGTATTTTCCACACCCAGATCCATGGAAAGGTCATGGC GATGGACAAAGTGAAAGTGAGAAGGAAGCCCAGGGAGCCACTGACAGGGGGCACCTTGAT GAAGAGGAGGTGATCGCCTCTGGTGTGGAGAATGCCTCAGGGATTTCTGAAAAAGGGCAG AGTGACCAGGAACTGTCTTCTCTGGTTGCATCTGAACATCAGGAAATCTGCATTAAATCA GGCAAAATCAGCTCTCTTGCTGTCACTTTCAGTCCTCAAACCGAGGAACCAGAGGAAGTT TTAGAGTATGAGGAGGGGTCTTACAATCTAGACTCCCGTGATGTGCAAACAGGGATGTCC GCAGATAACCTGCAGCCAAAAGATACCCATGAAAAACACCTCATGAGTCAAAGAAATTCA GGTGAAACTACTGAGACTTCAGATGGGATGAATTTCACAAAATATGTATCTGTACCTGAA AAGGATCTTGAGAAAACTGAAGAATGTAACTTTCTAGAGCCAGAGAACGTGGGTGGAGGG CCACCTCACAGAGTTCCCCGAAGTCTTGATTTTGGGGACGTCCCTATAGACAGTGATGTG CATGTCAGCAGCACATGTTCTGAGATAACCAAAAATCTTGACGTTAAGGGGTCTGAAAAT AGCCTTCCAGGAGCCGGTTCGTCTGGAAATTTTGACAGAGATACTATTTCTAGTGAGTAT ACTCATTCAAGTGCATCAAGTCCTGAGTTAAATGACTCTTCAGTTGCACTGTCCTCCTGG GGCCAGCAACCCAGTTCTGGGTATCAAGAAGAAAACCAAGGCAACTGGAGTGAACAGAAT CACCAAGAATCTGAACTAATTACCACTGATGGCCAAGTAGAAATAGTTACCAAAGTGAAG GATTTAGAGAAAAACAGAATAAATGAGTTTGAAAAGAGCTTTGATCGCAAAACTCCTACA TTTTTAGAGATCTGGAATGACTCAGTTGATGGTGATTCCTTTTCCTCTTTATCCAGTCCT GAAACAGGCAAATATTCTGAACATTCAGGGACACATCAGGAAAGCAATCTAATTGCTAGC TACCAGGAGAAAAATGAACATGACATTTCTGCAACTGTGCAGCCAGAGGATGCCAGGGTC ATTTCAACAAGCTCAGGTTCTGATGATGACAGTGTCGGTGGTGAAGAGTCAATAGAGGAA GAGATCCAGGTGGCCAACTGCCACGTTGCTGAGGATGAATCCAGAGCTTGGGATTCATTG AATGAATCTAATAAGTTCTTGGTCACAGCTGATCCTAAGTCTGAAAATATTTATGACTAC CTAGACAGCTCAGAGCCAGCAGAGAATGAGAATAAGTCAAACCCATTCTGTGACAATCAA CAAAGCAGCCCTGATCCCTGGACTTTCTCACCATTAACGGAGACTGAAATGCAGATTACA GCAGTGGAGAAGGAGAAGAGATCTTCTCCAGAAACAGGGACAACAGGAGATGTTGCATGG CAAATATCTCCCAAAGCTTCGTTCCCAAAGAACGAAGATAATTCTCAACTGGAAATGCTG GGCTTCTCAGCTGATAGCACTGAGTGGTGGAAGGCCTCACCCCAGGAAGGGAGACTAATT GAAAGTCCATTTGAAAGGGAGCTGTCTGACTCCAGTGGTGTGTTGGAGATAAATTCTTCA GTACACCAAAATGCCAGTCCCTGGGGAGTACCAGTTCAGGGTGATATTGAGCCCGTGGAA ACACACTATACTAATCCTTTTAGTGACAACCATCAGTCACCCTTTCTGGAAGGTAATGGG AAGAACTCCCATGAGCAACTCTGGAACATTCAACCAAGGCAGCCAGACCCAGATGCTGAC AAGTTCAGCCAGCTTGTAAAATTAGACCAAATTAAAGAAAAGGACTCAAGAGAGCAAACC TTTGTGTCTGCTGCTGGTGATGAGCTGACTCCTGAAACACCTACCCAAGAGCAGTGTCAG GACACCATGCTGCCAGTCTGTGATCATCCGGACACAGCCTTTACTCACGCAGAGGAAAAT AGTTGTGTTACATCTAATGTTTCAACTAATGAAGGTCAAGAAACAAATCAGTGGGAACAA GAAAAATCATACCTAGGTGAGATGACAAATTCAAGCATTGCCACAGAAAATTTTCCTGCT GTCAGTTCTCCCACCCAACTGATAATGAAGCCAGGCTCTGAATGGGATGGCTCTACCCCA AGTGAGGACTCCCGAGGTACCTTTGTGCCAGATATTTTACATGGCAACTTTCAAGAGGGT GGGCAGCTGGCCTCTGCCGCGCCTGACTTGTGGATAGATGCTAAGAAGCCCTTCAGTTTG AAAGCAGATGGTGAGAATCCTGATATCCTGACGCACTGCGAACATGACAGCAATTCTCAG GCTTCCGACAGCCCTGATATATGTCACGATTCTGAAGCAAAGCAAGAGACTGAGAAGCAC CTCAGTGCTTGCATGGGACCTGAAGTGGAATCCAGTGAGCTTTGTCTCACTGAGCCAGAG ATAGATGAAGAACCCATTTATGAGCCTGGACGGGAGTTTGTCCCATCCAATGCAGAACTC GATTCTGAAAACGCAACTGTGCTGCCTCCAATTGGCTATCAAGCAGACATAAAGGGCTCC TCTCAGCCCGCCTCTCATAAAGGTTCACCTGAACCTTCTGAAATAAACGGTGACAACAGT ACAGGTTTACAAGTATCAGAAAAAGGAGCCAGCCCAGATATGGCACCAATTTTGGAACCA GTTGACAGAAGAATCCCAAGGATTGAAAATGTGGCAACTAGCATTTTTGTAACTCACCAA GAGCCAACTCCAGAAGGTGACGGTTCTTGGATATCAGACAGCTTTTCTCCTGAAAGTCAG CCTGGTGCAAGAGCTTTGTTTGATGGTGATCCACATTTATCCACAGAGAATCCTGCCTTG GTTCCTGATGCTTTGCTAGCCTCAGACACTTGTCTGGATATAAGCGAAGCTGCCTTTGAC CACAGTTTCAGCGATGCCTCAGGTCTCAACACATCCACGGGAACAATAGATGACATGAGT AAACTGACATTATCCGAAGGCCATCCGGAAACGCCAGTTGATGGGGACCTAGGGAAGCAA GATATCTGCTCATCTGAAGCCTCGTGGGGTGATTTTGAATATGATGTAATGGGCCAGAAT ATCGATGAAGATTTACTGAGAGAGCCTGAACACTTCCTGTATGGTGGTGACCCTCCTTTG GAGGAAGATTCTCTGAAGCAGTCGCTGGCACCGTACACACCTCCCTTTGATTTGTCTTAT CTCACAGAACCTGCCCAGAGTGCTGAAACAATAGAGGAAGCTGGGTCTCCAGAGGATGAA TCTCTGGGATGCAGAGCAGCAGAGATAGTGCTTTCTGCACTTCCTGATCGAAGAAGTGAG GGAAACCAGGCTGAGACCAAAAACAGACTGCCTGGATCCCAGCTGGCTGTGCTGCATATT CGTGAAGACCCTGAGTCCGTTTATTTGCCGGTAGGAGCAGGCTCCAACATTTTGTCTCCA TCAAACGTTGACTGGGAAGTAGAAACAGATAATTCTGATTTACCAGCAGGTGGAGACATA GGACCACCAAATGGTGCCAGCAAGGAAATATCAGAATTGGAAGAAGAAAAAACAATTCCT ACCAAAGAGCCTGAGCAGATAAAATCAGAATACAAGGAAGAAAGATGTACAGAGAAGAAT GAAGATCGTCATGCACTACACATGGATTACATACTTGTAAACCGTGAAGAAAATTCACAC TCAAAGCCAGAGACCTGTGAAGAAAGAGAAAGCATAGCTGAATTAGAATTGTATGTAGGT TCCAAAGAAACAGGGCTGCAGGGAACTCAGTTAGCAAGCTTCCCAGACACATGTCAGCCA GCCTCCTTAAATGAAAGAAAAGGTCTCTCTGCAGAGAAAATGTCTTCTAAAAGCGATACG AGATCATCTTTTGAAAGCCCTGCACAAGACCAGAGTTGGATGTTCTTGGGCCATAGTGAG GTTGGTGATCCATCACTGGATGCCAGGGACTCAGGGCCTGGGTGGTCTGGCAAGACTGTG GAGCCGTTCTCTGAACTCGGCTTGGGTGAGGGTCCCCAGCTGCAGATTCTGGAAGAAATG AAGCCTCTAGAATCTTTGGCACTAGAGGAAGCCTCTGGTCCAGTCAGCCAATCACAGAAG AGTAAGAGCCGAGGCAGGGCTGGCCCGGATGCAGTTACGTTGCAGGCTGTCACCCATGAC AATGAATGGGAAATGCTTTCACCACAGCCTGTTCAGAAAAACATGATCCCTGACACGGAA ATGGAGGAGGAGACAGAGTTCCTTGAGCTCGGAACCAGGATATCAAGACCAAATGGACTA CTGTCAGAGGATGTAGGAATGGACATCCCCTTTGAAGAGGGCGTGCTGAGTCCCAGTGCT GCAGACATGAGGCCTGAACCTCCTAATTCTCTGGATCTTAATGACACTCATCCTCGGAGA ATCAAGCTCACAGCCCCAAATATCAATCTTTCTCTGGACCAAAGTGAAGGATCTATTCTC TCTGATGATAACTTGGACAGTCCAGATGAAATTGACATCAATGTGGATGAACTTGATACC CCCGATGAAGCAGATTCTTTTGAGTACACTGGCCATGATCCCACAGCCAACAAAGATTCT GGCCAAGAGTCAGAGTCTATTCCAGAATATACGGCCGAAGAGGAACGGGAGGACAACCGG CTTTGGAGGACAGTGGTCATTGGAGAACAAGAGCAGCGCATTGACATGAAGGTCATCGAG CCCTACAGGAGAGTCATTTCTCACGGAGGATACTATGGGGACGGTCTAAATGCCATCATT GTGTTTGCCGCCTGTTTTCTGCCAGACAGCAGTCGGGCGGATTACCACTATGTCATGGAA AATCTTTTCCTATATGTAATAAGTACTTTAGAGTTGATGGTAGCTGAAGACTATATGATT GTGTACTTGAATGGTGCAACCCCAAGAAGGAGGATGCCAGGGCTAGGCTGGATGAAGAAA TGCTACCAGATGATTGACAGACGGTTGAGGAAGAATTTGAAATCATTCATCATTGTTCAT CCATCTTGGTTCATCAGAACAATCCTTGCTGTGACACGACCTTTTATAAGTTCAAAATTC AGCAGTAAAATTAAATATGTCAATAGCTTATCAGAACTCAGTGGGCTGATCCCAATGGAT TGCATCCACATTCCAGAGAGCATCATCAAACTGGATGAAGAACTGAGGGAAGCATCAGAG GCAGCTAAAACTAGCTGCCTTTACAATGATCCAGAAATGTCTTCTATGGAGAAGGATATT GACTTGAAGCTGAAAGAAAAGCCT |
| Restriction Sites | Please inquire |
| ACCN | NM_015225 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015225.2, NP_056040.2 |
| RefSeq Size | 12602 bp |
| RefSeq ORF | 9267 bp |
| Locus ID | 158471 |
| UniProt ID | Q8WUY3 |
| Gene Summary | The protein encoded by this gene belongs to the B-cell CLL/lymphoma 2 and adenovirus E1B 19 kDa interacting family, whose members play roles in many cellular processes including apotosis, cell transformation, and synaptic function. Several functions for this protein have been demonstrated including suppression of Ras homolog family member A activity, which results in reduced stress fiber formation and suppression of oncogenic cellular transformation. A high molecular weight isoform of this protein has also been shown to colocalize with Adaptor protein complex 2, beta-Adaptin and endodermal markers, suggesting an involvement in post-endocytic trafficking. In prostate cancer cells, this gene acts as a tumor suppressor and its expression is regulated by prostate cancer antigen 3, a non-protein coding gene on the opposite DNA strand in an intron of this gene. Prostate cancer antigen 3 regulates levels of this gene through formation of a double-stranded RNA that undergoes adenosine deaminase actin on RNA-dependent adenosine-to-inosine RNA editing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. This RefSeq lacks full-length transcript support, but the extent of this transcript is supported by transcript alignments in human, aligning orthologous transcripts, and on functional support for the protein in PMID: 16288218. |
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