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RIPK4 (NM_020639) Human Untagged Clone
CAT＃: SC323692
RIPK4 (untagged)-Kinase deficient mutant (K51M) of Human receptor-interacting serine-threonine kinase 4 (RIPK4)
CNY 8,624.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ANKK2; ANKRD3; CHANDS; DIK; NKRD3; PKK; PPS2; RIP4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_020639, the custom clone sequence may differ by one or more nucleotides
ATGGAGGGCGACGGCGGGACCCCATGGGCCCTGGCGCTGCTGCGCACCTTCGACGCGGGCGAGTTCACGG GCTGGGAGAAGGTGGGCTCGGGCGGCTTCGGGCAGGTGTACAAGGTGCGCCATGTCCACTGGAAGACCTG GCTGGCCATCAAGTGCTCGCCCAGCCTGCACGTCGACGACAGGGAGCGCATGGAGCTTTTGGAAGAAGCC AAGAAGATGGAGATGGCCAAGTTTCGCTACATCCTGCCTGTGTATGGCATCTGCCGCGAACCTGTCGGCC TGGTCATGGAGTACATGGAGACGGGCTCCCTGGAAAAGCTGCTGGCTTCGGAGCCATTGCCATGGGATCT CCGGTTCCGAATCATCCACGAGACGGCGGTGGGCATGAACTTCCTGCACTGCATGGCCCCGCCACTCCTG CACCTGGACCTCAAGCCCGCGAACATCCTGCTGGATGCCCACTACCACGTCAAGATTTCTGATTTTGGTC TGGCCAAGTGCAACGGGCTGTCCCACTCGCATGACCTCAGCATGGATGGCCTGTTTGGCACAATCGCCTA CCTCCCTCCAGAGCGCATCAGGGAGAAGAGCCGGCTCTTCGACACCAAGCACGATGTATACAGCTTTGCG ATCGTCATCTGGGGCGTGCTCACACAGAAGAAGCCGTTTGCAGATGAGAAGAACATCCTGCACATCATGG TGAAGGTGGTGAAGGGCCACCGCCCCGAGCTGCCGCCCGTGTGCAGAGCCCGGCCGCGCGCCTGCAGCCA CCTGATACGCCTCATGCAGCGGTGCTGGCAGGGGGATCCGCGAGTTAGGCCCACCTTCCAAGAAATTACT TCTGAAACCGAGGACCTGTGTGAAAAGCCTGATGACGAAGTGAAAGAAACTGCTCATGATCTGGACGTGA AAAGCCCCCCGGAGCCCAGGAGCGAGGTGGTGCCTGCGAGGCTCAAGCGGGCCTCTGCCCCCACCTTCGA TAACGACTACAGCCTCTCCGAGCTGCTCTCACAGCTGGACTCTGGAGTTTCCCAGGCTGTCGAGGGCCCC GAGGAGCTCAGCCGCAGCTCCTCTGAGTCCAAGCTGCCATCGTCCGGCAGTGGGAAGAGGCTCTCGGGGG TGTCCTCGGTGGACTCCGCCTTCTCTTCCAGAGGATCACTGTCGCTGTCCTTTGAGCGGGAACCTTCAAC CAGCGATCTGGGCACCACAGACGTCCAGAAGAAGAAGCTTGTGGATGCCATCGTGTCCGGGGACACCAGC AAACTGATGAAGATCCTGCAGCCGCAGGACGTGGACCTGGCACTGGACAGCGGTGCCAGCCTGCTGCACC TGGCGGTGGAGGCCGGGCAAGAGGAGTGCGCCAAGTGGCTGCTGCTCAACAATGCCAACCCCAACCTGAG CAACCGTAGGGGCTCCACCCCGTTGCACATGGCCGTGGAGAGGAGGGTGCGGGGTGTCGTGGAGCTCCTG CTGGCGCGGAAGATCAGTGTCAACGCCAAGGATGAGGACCAGTGGACAGCCCTCCACTTTGCAGCCCAGA ACGGGGACGAGTCTAGCACACGGCTGCTGTTGGAGAAGAACGCCTCGGTCAACGAGGTGGACTTTGAGGG CCGGACGCCCATGCACGTGGCCTGCCAGCACGGGCAGGAGAATATCGTGCGCATCCTGCTGCGCCGAGGC GTGGACGTGAGCCTGCAGGGCAAGGATGCCTGGCTGCCACTGCACTACGCTGCCTGGCAGGGCCACCTGC CCATCGTCAAGCTGCTGGCCAAGCAGCCGGGGGTGAGTGTGAACGCCCAGACGCTGGATGGGAGGACGCC ATTGCACCTGGCCGCACAGCGCGGGCACTACCGCGTGGCCCGCATCCTCATCGACCTGTGCTCCGACGTC AACGTCTGCAGCCTGCTGGCACAGACACCCCTGCACGTGGCCGCGGAGACGGGGCACACGAGCACTGCCA GGCTGCTCCTGCATCGGGGCGCTGGCAAGGAGGCCATGACCTCAGACGGCTACACCGCTCTGCACCTGGC TGCCCGCAACGGACACCTGGCCACTGTCAAGCTGCTTGTCGAGGAGAAGGCCGATGTGCTGGCCCGGGGA CCCCTGAACCAGACGGCGCTGCACCTGGCTGCCGCCCACGGGCACTCGGAGGTGGTGGAGGAGTTGGTCA GCGCCGATGTCATTGACCTGTTCGACGAGCAGGGGCTCAGCGCGCTGCACCTGGCCGCCCAGGGCCGGCA CGCACAGACGGTGGAGACTCTGCTCAGGCATGGGGCCCACATCAACCTGCAGAGCCTCAAGTTCCAGGGC GGCCATGGCCCCGCCGCCACGCTCCTGCGGCGAAGCAAGACCTAG >OriGene 5' read for mutant NM_020639 unedited
CCCGCCGTTCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGACGGCGGGACCC CATGGGCCCTGGCGCTGCTGCGCACCTTCGACGCGGGCGAGTTCACGGGCTGGGAGAAGGTGGGCTCGGG CGGCTTCGGGCAGGTGTACAAGGTGCGCCATGTCCACTGGAAGACCTGGCTGGCCATCATGTGCTCGCCC AGCCTGCACGTCGACGACAGGGAGCGCATGGAGCTTTTTGGAAGAAGCCAAGAAAGATGGGAGATGGGCC AAGTTTTCGCTACATCCCTGCCCTGTGTATGGGCATCTGCCGCGAAACCTGTCGGCCTGTTCATGGGAGT ACATGGGAACAGGCTCCCCTGAAAAAGCTGCTGGGCTTCCGAACCATTTGCATGGGGATTCTCCGTTTCG GATTCATCCACAAAACGGCGTTGGCATTGAACTTCTGGAATTGCAGGCCCCCGCAATTCCGCGAACCTGA CCTTCAGGCCCGGACATTCTGTTGAATGCCAATTCACCGCCTAGAGATCTCTGATTTGGCTCGGGCAAGT TGACACGGGTGTGTCCCACCGGCGAGACCTAGACAGGAGATGCCCGGTTGGCAAACGCGTCATCTTCTAC AGAGCATTCGAGAGAGACCGGGCTTCTAAACCAAACACAGTATAAAACGTTTCGATATCACTCGGGCGGC TCACAGAGAACGCTTTGATAGAGAAACTCTGCACACTGTGAAGTGTGAAGCCGCCCAATCCCCGTGTGAA CGCGCCTGAACTGGCTTACAGCTGCAAGATCAATTAACTCGAACTTTGAGCACGTGAAACTGCGAGTGAG GCTGACTGGT >SC323692 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSAAKGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGACGGCGGGACCCCATG GGCCCTGGCGCTGCTGCGCACCTTCGACGCGGGCGAGTTCACGGGCTGGGAGAAGGTGGGCTCGGGCGGC TTCGGGCAGGTGTACAAGGTGCGCCATGTCCACTGGAAGACCTGG |
| Restriction Sites | Please inquire |
| ACCN | NM_020639 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020639.2, NP_065690.2 |
| RefSeq Size | 3890 bp |
| RefSeq ORF | 2355 bp |
| Locus ID | 54101 |
| Domains | pkinase, TyrKc, ANK, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene is a serine/threonine protein kinase that interacts with protein kinase C-delta. The encoded protein can also activate NFkappaB and is required for keratinocyte differentiation. This kinase undergoes autophosphorylation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214342 | RIPK4 (Myc-DDK-tagged)-Human receptor-interacting serine-threonine kinase 4 (RIPK4) |
CNY 8,616.00 |
|
| RC214342L1 | Lenti-ORF clone of RIPK4 (Myc-DDK-tagged)-Human receptor-interacting serine-threonine kinase 4 (RIPK4) |
CNY 11,016.00 |
|
| RC214342L2 | Lenti-ORF clone of RIPK4 (mGFP-tagged)-Human receptor-interacting serine-threonine kinase 4 (RIPK4) |
CNY 5,890.00 |
|
| RC214342L3 | Lenti-ORF clone of RIPK4 (Myc-DDK-tagged)-Human receptor-interacting serine-threonine kinase 4 (RIPK4) |
CNY 11,016.00 |
|
| RC214342L4 | Lenti-ORF clone of RIPK4 (mGFP-tagged)-Human receptor-interacting serine-threonine kinase 4 (RIPK4) |
CNY 11,016.00 |
|
| RG214342 | RIPK4 (tGFP-tagged) - Human receptor-interacting serine-threonine kinase 4 (RIPK4) |
CNY 10,216.00 |
|
| SC310116 | RIPK4 (untagged)-Human receptor-interacting serine-threonine kinase 4 (RIPK4) |
CNY 8,624.00 |
