MED8 (NM_001001653) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARC32
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331121 representing NM_001001653. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCGGCAGACTGAAGGACGGGTGCCTGTTTTCAGCCATGAGGTAGTCCCTGACCATCTGAGAACCAAG CCTGACCCTGAAGTGGAAGAACAGGAGAAGCAACTGACGACAGATGCTGCCCGCATTGGTGCAGATGCA GCCCAGAAGCAGATCCAGAGCTTGAATAAAATGTGTTCAAACCTTCTGGAGAAAATCAGCAAAGAGGAG CGAGAATCAGAGAGTGGAGGTCTCCGGCCGAACAAGCAGACCTTTAACCCTACAGACACTAATGCCTTG GTGGCAGCTGTTGCCTTTGGGAAAGGACTATCTAATTGGAGACCTTCAGGCAGCAGTGGTCCTGGCCAG GCAGGCCAGCCAGGAGCTGGGACGATCCTTGCAGGAACCTCAGGATTACAGCAGGTGCAGATGGCAGGA GCTCCAAGCCAGCAGCAGCCAATGCTCAGTGGGGTACAAATGGCTCAGGCAGGTCAACCAGGGAAAATG CCAAGTGGAATAAAAACCAACATCAAGTCGGCTTCCATGCATCCCTACCAGCGGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001001653
Insert Size	540 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001001653.2
RefSeq Size	2032 bp
RefSeq ORF	540 bp
Locus ID	112950
UniProt ID	Q96G25
Protein Families	Druggable Genome, Transcription Factors
MW	19 kDa
Gene Summary	This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (4) contains multiple differences in the UTRs and coding region, compared to variant 3. It initiates translation at a downstream in-frame start codon. The encoded isoform (1) has shorter N- and C-termini, compared to isoform 3.

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