MYBPC1 (NM_001254718) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LCCS4; MYBPCC; MYBPCS; MYOTREM; ssMyBP-C
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332258 representing NM_001254718. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCAGAACCCACTAAGAAAGAGGAAAATGAAGTGCCAGCCCCAGCCCCACCCCCGGAAGAACCAAGT AAAGAGAAGGAGGCCGGAACTACACCAGCAAAAGACTGGACCCTTGTCGAAACTCCTCCTGGGGAGGAA CAAGCCAAGCAGAATGCCAACTCCCAGCTGTCCATCTTGTTCATTGAAAAACCTCAAGGAGGAACAGTG AAAGTTGGTGAAGATATCACCTTCATAGCCAAAGTCAAGGCTGAAGATCTTCTGAGAAAACCCACTATC AAATGGTTCAAAGGAAAATGGATGGACCTGGCCAGCAAAGCCGGGAAGCACCTTCAGCTGAAGGAAACC TTTGAGAGGCACAGTCGGGTGTACACATTTGAGATGCAGATCATCAAGGCCAAAGATAACTTTGCAGGA AATTACAGATGCGAGGTCACCTATAAGGATAAGTTTGACAGCTGTTCATTTGATCTTGAAGTGCACGAA TCTACTGGGACTACTCCAAACATTGACATCAGATCTGCTTTCAAGAGAAGTGGAGAAGGTCAAGAGGAT GCAGGAGAACTTGACTTTAGTGGTCTCCTGAAACGTAGGGAGGTGAAGCAGCAGGAGGAAGAACCCCAG GTGGACGTATGGGAGTTGCTGAAGAACGCGAAACCCAGTGAGTACGAGAAGATCGCCTTCCAGTATGGA ATCACCGACCTGCGCGGCATGCTCAAGCGACTCAAGCGCATGCGCAGAGAGGAGAAGAAGAGCGCAGCT TTTGCAAAAATTCTTGATCCTGCATATCAGGTTGACAAAGGAGGCAGAGTGAGGTTTGTTGTGGAGCTG GCAGATCCAAAGTTGGAGGTGAAATGGTATAAAAATGGTCAAGAAATTCGACCCAGTACCAAATACATC TTTGAACACAAAGGATGCCAGAGAATCCTGTTTATCAATAACTGTCAGATGACAGATGATTCAGAGTAT TATGTGACAGCCGGTGATGAGAAATGTTCCACTGAGCTCTTCGTAAGAGAGCCTCCAATTATGGTGACC AAACAGCTGGAAGATACAACTGCTTATTGTGGGGAGAGAGTGGAATTAGAATGTGAGGTGTCTGAAGAT GATGCCAATGTAAAATGGTTTAAGAATGGTGAAGAGATTATCCCTGGTCCAAAATCAAGATACCGAATT AGAGTTGAGGGTAAAAAACACATCTTGATCATAGAGGGAGCAACAAAGGCTGATGCTGCAGAATATTCA GTAATGACAACAGGAGGACAATCATCTGCTAAACTTAGTGTTGACTTGAAACCTCTGAAGATTTTGACA CCTCTGACTGATCAGACTGTAAATCTTGGAAAAGAAATCTGCCTGAAGTGTGAAATCTCTGAAAACATA CCAGGAAAATGGACTAAAAATGGCCTACCTGTTCAGGAGAGTGACCGTCTAAAGGTGGTTCACAAGGGA AGGATCCACAAGTTAGTGATAGCCAATGCCCTCACTGAAGATGAAGGTGATTATGTATTTGCACCTGAT GCCTACAATGTTACTCTGCCTGCCAAAGTTCATGTTATTGATCCTCCTAAGATCATCCTGGATGGTCTT GATGCTGACAACACAGTGACAGTGATTGCAGGAAACAAGCTTCGTCTTGAGATCCCCATCAGCGGAGAA CCACCTCCTAAAGCCATGTGGAGCCGGGGAGATAAGGCTATTATGGAAGGCAGTGGCCGGATAAGAACA GAATCTTACCCTGATAGCAGCACTCTGGTCATTGATATAGCTGAAAGAGATGACTCTGGTGTTTACCAC ATCAATCTGAAAAACGAAGCTGGAGAGGCACATGCAAGCATCAAGGTTAAAGTTGTGGACTTCCCTGAT CCTCCAGTGGCACCGACTGTGACAGAGGTGGGAGATGACTGGTGTATCATGAACTGGGAGCCTCCTGCC TACGACGGAGGCTCTCCAATCCTAGGATATTTTATTGAGAGGAAGAAGAAACAAAGCTCCAGGTGGATG AGGCTGAATTTTGATCTCTGCAAAGAAACAACTTTTGAGCCCAAGAAGATGATTGAAGGTGTGGCCTAT GAGGTCCGCATCTTTGCAGTCAATGCCATTGGCATCTCCAAGCCCAGTATGCCCTCCAGGCCTTTTGTT CCTTTGGCTGTAACAAGCCCTCCTACTCTTCTGACTGTGGACTCTGTCACTGACACGACTGTCACGATG AGGTGGCGCCCCCCAGACCACATTGGTGCAGCAGGTTTAGATGGCTATGTGCTAGAGTATTGCTTTGAA GGAAGTACATCAGCAAAACAGTCTGATGAAAATGGGGAGGCTGCCTATGATCTGCCAGCTGAGGACTGG ATAGTTGCAAACAAAGATCTGATTGACAAGACGAAGTTCACCATCACAGGTCTGCCAACAGATGCAAAG ATCTTTGTGCGTGTGAAGGCTGTTAATGCAGCTGGTGCCAGCGAGCCCAAGTACTATTCTCAGCCCATT CTCGTGAAGGAAATCATAGAACCTCCAAAGATTCGCATTCCAAGACACCTGAAGCAAACCTATATCCGC AGAGTTGGAGAAGCTGTCAATCTGGTTATACCTTTCCAGGGAAAACCAAGACCAGAATTAACTTGGAAG AAGGATGGTGCAGAAATTGATAAGAATCAAATAAACATTCGCAACTCTGAGACTGATACAATCATATTT ATTAGAAAAGCAGAGAGGAGCCACTCTGGGAAATATGATCTGCAAGTCAAAGTGGACAAATTCGTGGAG ACCGCATCAATTGACATCCAGATCATTGACCGTCCAGGTCCACCCCAAATTGTGAAGATTGAGGATGTC TGGGGAGAAAATGTCGCTCTCACATGGACTCCACCAAAGGATGATGGAAATGCTGCTATCACAGGCTAT ACCATTCAGAAGGCTGACAAGAAGAGCATGGAATGGTTTACTGTCATTGAGCATTATCATCGAACCAGT GCCACCATTACTGAATTGGTCATAGGGAATGAATATTACTTCCGGGTCTTTTCTGAAAACATGTGTGGC CTCAGTGAGGATGCCACCATGACTAAAGAGAGTGCAGTGATCGCCAGGGATGGTAAAATCTACAAAAAT CCAGTGTATGAAGACTTTGATTTCTCAGAGGCACCCATGTTTACTCAGCCTTTGGTTAACACCTATGCC ATAGCTGGTTACAATGCCACCCTAAACTGCAGTGTGAGAGGAAATCCTAAGCCTAAAATAACCTGGATG AAAAACAAAGTTGCTATTGTGGATGATCCAAGATACAGGATGTTCAGCAACCAGGGAGTCTGTACCCTG GAAATTCGCAAGCCCAGCCCCTATGATGGAGGCACTTACTGCTGCAAAGCAGTCAATGACCTTGGGACA GTGGAGATTGAATGCAAACTGGAGGTGAAAGTGATATATCAAGGAGTAAATACCCCTGGACAACCAGTC TTCCTGGAGGGGCAGCAACAGGTGGGCTCTCCTTCTGCAGACTCCTCTTGCAAGGCGTACCTCCAAACA TAA
Restriction Sites	SgfI-MluI
ACCN	NM_001254718
Insert Size	3522 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001254718.1
RefSeq Size	3930 bp
RefSeq ORF	3522 bp
Locus ID	4604
UniProt ID	Q00872
MW	131.7 kDa
Gene Summary	This gene encodes a member of the myosin-binding protein C family. Myosin-binding protein C family members are myosin-associated proteins found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The encoded protein is the slow skeletal muscle isoform of myosin-binding protein C and plays an important role in muscle contraction by recruiting muscle-type creatine kinase to myosin filaments. Mutations in this gene are associated with distal arthrogryposis type I. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (5) lacks three exons and includes an alternate exon in the coding region, compared to variant 1. The encoded isoform (5) is longer and has a distinct C-terminus, compared to isoform 1.

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