MYBPC1 (NM_001254721) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LCCS4; MYBPCC; MYBPCS; MYOTREM; ssMyBP-C
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332261 representing NM_001254721. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCAGAACCCACTAAGAAAGAGGAAAATGAAGTGCCAGCCCCAGCCCCACCCCCGGAAGAACCAAGT AAAGAGAAGGAGGCCGGAACTACACCAGCAAAAGACTGGACCCTTGTCGAAACTCCTCCTGGGGAGGAA CAAGCCAAGCAGAATGCCAACTCCCAGCTGTCCATCTTGTTCATTGAAAAACCTCAAGGAGGAACAGTG AAAGTTGGTGAAGATATCACCTTCATAGCCAAAGTCAAGGCTGAAGATCTTCTGAGAAAACCCACTATC AAATGGTTCAAAGGAAAATGGATGGACCTGGCCAGCAAAGCCGGGAAGCACCTTCAGCTGAAGGAAACC TTTGAGAGGCACAGTCGGGTGTACACATTTGAGATGCAGATCATCAAGGCCAAAGATAACTTTGCAGGA AATTACAGATGCGAGGTCACCTATAAGGATAAGTTTGACAGCTGTTCATTTGATCTTGAAGTGCACGAA TCTACTGGGACTACTCCAAACATTGACATCAGATCTGCTTTCAAGAGAAGGGAGGTGAAGCAGCAGGAG GAAGAACCCCAGGTGGACGTATGGGAGTTGCTGAAGAACGCGAAACCCAGTGAGTACGAGAAGATCGCC TTCCAGTATGGAATCACCGACCTGCGCGGCATGCTCAAGCGACTCAAGCGCATGCGCAGAGAGGAGAAG AAGAGCGCAGCTTTTGCAAAAATTCTTGATCCTGCATATCAGGTTGACAAAGGAGGCAGAGTGAGGTTT GTTGTGGAGCTGGCAGATCCAAAGTTGGAGGTGAAATGGTATAAAAATGGTCAAGAAATTCGACCCAGT ACCAAATACATCTTTGAACACAAAGGATGCCAGAGAATCCTGTTTATCAATAACTGTCAGATGACAGAT GATTCAGAGTATTATGTGACAGCCGGTGATGAGAAATGTTCCACTGAGCTCTTCGTAAGAGAGCCTCCA ATTATGGTGACCAAACAGCTGGAAGATACAACTGCTTATTGTGGGGAGAGAGTGGAATTAGAATGTGAG GTGTCTGAAGATGATGCCAATGTAAAATGGTTTAAGAATGGTGAAGAGATTATCCCTGGTCCAAAATCA AGATACCGAATTAGAGTTGAGGGTAAAAAACACATCTTGATCATAGAGGGAGCAACAAAGGCTGATGCT GCAGAATATTCAGTAATGACAACAGGAGGACAATCATCTGCTAAACTTAGTGTTGACTTGAAACCTCTG AAGATTTTGACACCTCTGACTGATCAGACTGTAAATCTTGGAAAAGAAATCTGCCTGAAGTGTGAAATC TCTGAAAACATACCAGGAAAATGGACTAAAAATGGCCTACCTGTTCAGGAGAGTGACCGTCTAAAGGTG GTTCACAAGGGAAGGATCCACAAGTTAGTGATAGCCAATGCCCTCACTGAAGATGAAGGTGATTATGTA TTTGCACCTGATGCCTACAATGTTACTCTGCCTGCCAAAGTTCATGTTATTGATCCTCCTAAGATCATC CTGGATGGTCTTGATGCTGACAACACAGTGACAGTGATTGCAGGAAACAAGCTTCGTCTTGAGATCCCC ATCAGCGGAGAACCACCTCCTAAAGCCATGTGGAGCCGGGGAGATAAGGCTATTATGGAAGGCAGTGGC CGGATAAGAACAGAATCTTACCCTGATAGCAGCACTCTGGTCATTGATATAGCTGAAAGAGATGACTCT GGTGTTTACCACATCAATCTGAAAAACGAAGCTGGAGAGGCACATGCAAGCATCAAGGTTAAAGTTGTG GACTTCCCTGATCCTCCAGTGGCACCGACTGTGACAGAGGTGGGAGATGACTGGTGTATCATGAACTGG GAGCCTCCTGCCTACGACGGAGGCTCTCCAATCCTAGGATATTTTATTGAGAGGAAGAAGAAACAAAGC TCCAGGTGGATGAGGCTGAATTTTGATCTCTGCAAAGAAACAACTTTTGAGCCCAAGAAGATGATTGAA GGTGTGGCCTATGAGGTCCGCATCTTTGCAGTCAATGCCATTGGCATCTCCAAGCCCAGTATGCCCTCC AGGCCTTTTGTTCCTTTGGCTGTAACAAGCCCTCCTACTCTTCTGACTGTGGACTCTGTCACTGACACG ACTGTCACGATGAGGTGGCGCCCCCCAGACCACATTGGTGCAGCAGGTTTAGATGGCTATGTGCTAGAG TATTGCTTTGAAGGAACTGAGGACTGGATAGTTGCAAACAAAGATCTGATTGACAAGACGAAGTTCACC ATCACAGGTCTGCCAACAGATGCAAAGATCTTTGTGCGTGTGAAGGCTGTTAATGCAGCTGGTGCCAGC GAGCCCAAGTACTATTCTCAGCCCATTCTCGTGAAGGAAATCATAGAACCTCCAAAGATTCGCATTCCA AGACACCTGAAGCAAACCTATATCCGCAGAGTTGGAGAAGCTGTCAATCTGGTTATACCTTTCCAGGGA AAACCAAGACCAGAATTAACTTGGAAGAAGGATGGTGCAGAAATTGATAAGAATCAAATAAACATTCGC AACTCTGAGACTGATACAATCATATTTATTAGAAAAGCAGAGAGGAGCCACTCTGGGAAATATGATCTG CAAGTCAAAGTGGACAAATTCGTGGAGACCGCATCAATTGACATCCAGATCATTGACCGTCCAGGTCCA CCCCAAATTGTGAAGATTGAGGATGTCTGGGGAGAAAATGTCGCTCTCACATGGACTCCACCAAAGGAT GATGGAAATGCTGCTATCACAGGCTATACCATTCAGAAGGCTGACAAGAAGAGCATGGAATGGTTTACT GTCATTGAGCATTATCATCGAACCAGTGCCACCATTACTGAATTGGTCATAGGGAATGAATATTACTTC CGGGTCTTTTCTGAAAACATGTGTGGCCTCAGTGAGGATGCCACCATGACTAAAGAGAGTGCAGTGATC GCCAGGGATGGTAAAATCTACAAAAATCCAGTGTATGAAGACTTTGATTTCTCAGAGGCACCCATGTTT ACTCAGCCTTTGGTTAACACCTATGCCATAGCTGGTTACAATGCCACCCTAAACTGCAGTGTGAGAGGA AATCCTAAGCCTAAAATAACCTGGATGAAAAACAAAGTTGCTATTGTGGATGATCCAAGATACAGGATG TTCAGCAACCAGGGAGTCTGTACCCTGGAAATTCGCAAGCCCAGCCCCTATGATGGAGGCACTTACTGC TGCAAAGCAGTCAATGACCTTGGGACAGTGGAGATTGAATGCAAACTGGAGGTGAAAGTGATATATCAA GGAGTAAATACCCCTGGACAACCAGTCTTCCTGGAGGGGCAGCAACAGTCATTGCACAATAAGGATTTT TGA
Restriction Sites	SgfI-MluI
ACCN	NM_001254721
Insert Size	3384 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001254721.1
RefSeq Size	3862 bp
RefSeq ORF	3384 bp
Locus ID	4604
UniProt ID	Q00872
MW	127.1 kDa
Gene Summary	This gene encodes a member of the myosin-binding protein C family. Myosin-binding protein C family members are myosin-associated proteins found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The encoded protein is the slow skeletal muscle isoform of myosin-binding protein C and plays an important role in muscle contraction by recruiting muscle-type creatine kinase to myosin filaments. Mutations in this gene are associated with distal arthrogryposis type I. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (8) lacks three exons in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (8) is shorter than isoform 1.

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