ENT2 (SLC29A2) (NM_001300869) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DER12; ENT2; HNP36
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335616 representing NM_001300869. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGCGAGGAGACGCCCCGCGGGACAGCTACCACCTGGTCGGGATCAGCTTCTTCATCCTGGGGCTG GGCACCCTCCTTCCCTGGAACTTCTTCATCACCGCCATCCCGTACTTCCAGGCGCGACTGGCCGGGGCC GGCAACAGCACAGCCAGGATCCTGAGCACCAACCACACGGGTCCCGAGGATGCCTTCAACTTCAACAAT TGGGTGACGCTGCTGTCCCAGCTGCCCCTGCTGCTCTTCACCCTCCTCAACTCCTTCCTGTACCAGTGC GTCCCGGAGACGGTGCGCATTCTGGGCAGCCTGCTGGCCATACTGCTGCTCTTTGCCCTGACAGCAGCG CTGGTCAAGGTGGACATGAGCCCCGGACCCTTCTTCTCCATCACCATGGCCTCCGTCTGCTTCATCAAC TCCTTCAGTGCAGTCCTACAGGGCAGCCTCTTCGGGCAGCTGGGCACCATGCCCTCCACCTACAGCACC CTCTTCCTCAGCGGCCAGGGCCTGGCTGGGATCTTTGCTGCCCTTGCCATGCTCCTGTCCATGGCCAGT GGCGTGGACGCCGAGACCTCTGCCCTGGGGTACTTTATCACGCCCTGTGTGGGCATCCTCATGTCCATC GTGTGTTACCTGAGCCTGCCTCACCTGAAGTTTGCCCGCTACTACCTGGCCAATAAATCATCCCAGGCC CAAGCTCAGGAGCTGGAGACCAAAGCTGAGCTCCTCCAGTCTGATCTGGCTGACAGCGCTGTGCCTTGT GTTGGTCTTCACAGTCACCCTGTCCGTCTTCCCCGCCATCACAGCCATGGTGACCAGCTCCACCAGTCC TGGGAAGTGGAGTCAGTTCTTCAACCCCATCTGCTGCTTCCTCCTCTTCAACATCATGGACTGGCTGGG ACGGAGCCTGACCTCTTACTTCCTGTGGCCAGACGAGGACAGCCGGCTGCTGCCCCTGCTGGTCTGCCT GCGGTTCCTGTTCGTGCCCCTCTTCATGCTGTGCCACGTGCCCCAGAGGTCCCGGCTGCCCATCCTCTT CCCACAGGATGCCTACTTCATCACCTTCATGCTGCTCTTTGCCGTTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300869
Insert Size	1086 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300869.1
RefSeq Size	2561 bp
RefSeq ORF	1086 bp
Locus ID	3177
Protein Families	Transmembrane
MW	38.8 kDa
Gene Summary	The uptake of nucleosides by transporters, such as SLC29A2, is essential for nucleotide synthesis by salvage pathways in cells that lack de novo biosynthetic pathways. Nucleoside transport also plays a key role in the regulation of many physiologic processes through its effect on adenosine concentration at the cell surface (Griffiths et al., 1997 [PubMed 9396714]).[supplied by OMIM, Nov 2008] Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate exon compared to variant 1, that causes a frameshift. The resulting isoform (b) has a shorter and distinct C-terminus compared to isoform a.

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