PNMA3 (NM_001282535) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MA3; MA5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336224 representing NM_001282535. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCGTTGACCTTGTTACAGGACTGGTGTCGGGGGGAACACCTGAACACCCGGAGGTGCATGCTCATC CTGGGGATCCCCGAGGACTGTGGCGAGGATGAGTTTGAGGAGACACTCCAGGAGGCTTGCAGGCACCTG GGCAGATACAGGGTGATTGGCAGGATGTTTAGGAGGGAGGAGAACGCCCAGGCGATTCTACTGGAGCTG GCACAAGATATCGACTATGCTTTGCTCCCAAGGGAAATACCAGGAAAGGGGGGGCCCTGGGAAGTGATT GTAAAACCCCGTAACTCAGATGGGGAATTTCTCAACAGACTGAACCGCTTCTTAGAGGAGGAGAGGCGG ACCGTGTCAGATATGAACCGAGTCCTCGGGTCGGACACCAATTGTTCGGCTCCAAGAGTGACTATATCA CCAGAGTTCTGGACCTGGGCCCAGACTCTGGGGGCAGCAGTGCAGCCTCTGCTAGAACAAATGTTGTAC CGAGAACTAAGAGTGTTTTCTGGGAACACCATATCCATCCCAGGTGCACTGGCCTTTGATGCCTGGCTT GAGCACACCACTGAGATGCTACAGATGTGGCAGGTGCCCGAGGGGGAAAAGAGGCGGAGGCTGATGGAA TGCTTACGGGGCCCTGCTCTCCAGGTGGTCAGTGGGCTCCGGGCCAGCAATGCTTCCATAACTGTGGAG GAGTGCCTGGCTGCCTTGCAGCAGGTGTTCGGACCTGTGGAGAGCCATAAAATTGCCCAGGTGAAGTTG TGTAAAGCCTATCAGGAGGCAGGAGAGAAAGTATCTAGCTTTGTGTTACGTTTGGAACCCCTGCTCCAA AGAGCTGTAGAAAACAATGTGGTATCACGTAGAAACGTGAATCAGACTCGCCTGAAACGAGTCTTAAGT GGGGCCACCCTTCCTGACAAACTCCGAGATAAGCTTAAGCTGATGAAACAGCGAAGGAAGCCTCCTGGT TTCCTGGCCCTGGTGAAGCTCCTGCGTGAGGAGGAGGAATGGGAGGCCACTTTAGGTCCAGATAGGGAG AGTCTGGAGGGGCTGGAAGTAGCCCCAAGGCCACCTGCCAGGATCACTGGGGTTGGGGCAGTACCTCTC CCTGCCTCTGGCAACAGTTTTGATGCGAGGCCTTCCCAGGGCTACCGGCGCCGGAGGGGCAGAGGCCAA CACCGAAGGGGTGGTGTGGCAAGGGCTGGCTCTCGAGGCTCAAGAAAACGGAAACGCCACACATTCTGC TATAGCTGTGGGGAAGACGGCCACATCAGGGTACAGTGCATCAACCCCTCCAACCTGCTCTTGGCCAAG GAGACAAAAGAGATATTGGAAGGAGGGGAAAGAGAAGCCCAGACAAACAGCAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282535
Insert Size	1368 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282535.1
RefSeq Size	3647 bp
RefSeq ORF	1368 bp
Locus ID	29944
UniProt ID	Q9UL41
MW	51.5 kDa
Gene Summary	The protein encoded by this gene belongs to the paraneoplastic antigen MA (PNMA) family, which shares homology with retroviral Gag proteins. The PNMA antigens are highly expressed in the brain and also in a range of tumors associated with serious neurological phenotypes. PMID:16407312 reports the presence of a functional -1 ribosomal frameshift signal (consisting of a heptanucleotide shift motif followed 3' by a pseudoknot structure) in this gene, however, the frame-shifted product has not been characterized. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (2) is alternatively spliced in the 3' coding region, which results in a frame-shift compared to variant 1. The resulting isoform (2) is shorter with a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.