AER61 (EOGT) (NM_001278689) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AER61; AOS4; C3orf64; EOGT1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336622 representing NM_001278689. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTAATGTTGTTTGTCTTTGGAGTCTTACTTCATGAAGTCTCACTGAGTGGTCAGAATGAAGCTCCT CCTAATACTCACAGCATTCCAGGCGAACCTCTGTATAACTATGCCAGCATCCGCTTGCCAGAGGAGCAC ATTCCCTTCTTTTTGCACAACAATAGGCATATTGCCACTGTCTGTAGGAAAGACTCTCTTTGTCCATAT AAGAAACACCTAGAGAAGCTAAAGTACTGCTGGGGTTATGAGAAATCCTGCAAACCAGAGTTCAGGTTT GGTTACCCAGTTTGCAGCTATGTCGACATGGGATGGACGGACACTCTTGAGTCAGCTGAGGACATATTT TGGAAACAAGCTGACTTTGGATATGCCAGAGAGAGGCTGGAGGAGATGCATGTGCTCTGTCAGCCTAAG GAAACGAGTGACTCAAGTCTGGTGTGTTCCCGTTATCTTCAGTACTGCAGAGCAACCAATCTCTATCTT GATTTAAGAAACATCAAGAGAAATCATGACAGATTTAAGGAGGACTTTTTCCAGAGTGGTGAAATTGGA GGGCACTGTAAACTTGACATCCGTACATTGACGTCTGAAGGTCAGCGCAAAAGCCCTCTGCAGTCATGG TTTGCTGAGCTACAAAGCTATACTCAGCTCAACTTCAGACCTATAGAAGATGCTAAATGTGACATTGTC ATTGAAAAACCAACATATTTCATGAAATTAGATGCAGGTGTTAACATGTATCACCACTTCTGTGATTTC ATCAATCTTTATATTACTCAGCACGTTAATAACTCATTCAGTACTGACGTGTACATCGTGATGTGGGAC ACCAGTTCTTACGGATATGGTGACCTATTCTCCGACACATGGAATGCATTTACTGATTATGACGTTATA CATTTGAAAACTTATGATTCCAAAAGGGTATGTTTTAAAGAAGCTGTTTTTTCATTACTCCCCCGCATG AGGTATGGGCTGTTCTATAATACTCCTCTGATATCTGGCTGTCAAAATACTGGACTATTCAGGGCATTT GCCCAGCATGTACTACACAGACTAAACATCACACAAGAAGGACCTAAGGATGGAAAAATTCGAGTCACC ATTCTTGCACGGAGCACAGAATACCGGAAAATCCTTAACCAAAATGAGCTTGTAAATGCACTGAAAACA GTATCTACATTTGAAGTCCAGATTGTTGATTACAAGTATAGAGAACTTGGGTTTTTAGATCAACTAAGG ATCACACACAACACGGACATATTTATTGGAATGCATGGAGCTGGTCTGACCCATTTACTTTTCCTTCCA GACTGGGCTGCTGTATTTGAACTGTACAACTGTGAAGATGAACGCTGTTACTTAGACTTGGCCAGGCTG AGAGGCGTTCACTACATCACTTGGCGACGGCAGAACAAAGTCTTTCCTCAGGATAAGGGCCACCATCCA ACCCTGGGGGAGCACCCGAAGTTCACCAACTACTCTTTCGATGTAGAAGAATTTATGTATCTTGTCCTT CAGGCTGCAGACCACGTATTGCAACACCCAAAGTGGCCATTTAAGAAGAAACATGATGAGCTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278689
Insert Size	1584 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278689.1
RefSeq Size	4666 bp
RefSeq ORF	1584 bp
Locus ID	285203
UniProt ID	Q5NDL2
MW	62 kDa
Gene Summary	This gene encodes an enzyme that acts in the lumen of the endoplasmic reticulum to catalyze the transfer of N-acetylglucosamine to serine or threonine residues of extracellular-targeted proteins. This enzyme modifies proteins containing eukaryotic growth factor (EGF)-like domains, including the Notch receptor, thereby regulating developmental signalling. Mutations in this gene have been observed in individuals with Adams-Oliver syndrome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).

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