BAHD1 (NM_001301132) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301132, the custom clone sequence may differ by one or more nucleotides ATGACACACACTCGGAGAAAGTCCCTTCCCATGCTGAGTTCGGGCCTCACTGGCCGCCGAGAGCCCCTGC AGATGGAAGACAGCAACATGGAGCAGGGGGTTGAGGGTGTGGAGCCAGGCATGCCCGAGAGCCCAGGTCA CCTCACAGGGCGCCGCAAGAATTACCCACTTCGTAAGCGCCCATTGGTTCCTGAGAAGCCCAAGGCCTGC AAAGTGCTGCTGACTCGCCTGGAGAATGTGGCCGGTCCCCGGAGTGCAGATGAGGCTGATGAGCTACCGC CTGACCTGCCCAAGCCCCCCAGCCCGGCCCCATCCAGTGAAGACCCTGGCCTTGCCCAGCCCCGCAAGCG GCGCCTGGCCTCCCTCAATGCTGAAGCTCTCAATAACCTGCTGCTGGAGCGAGAGGACACCAGCAGCCTG GCAGGCACCCGCCGCAGTCGAGCAGGGGATCCCCACCGCAGCCGTGACCGTGATCGTGCTACTGGGGGCT GGTCCTCCTCCAAGAAGCGGCCCCGGCTGGGGGACCTTGGAGGAGGAAGTCGGGACCTGTCTCCAGAGCC AGCACCCGATGAAGGTCCCCGCCGAGATGGAGACCCAGCTCCCAAGAGACTGGCTAGCCTGAACGCAGCT GCTTTCCTAAAACTGAGCCAGGAGCGGGAGCTACCCCTGCGGCTGCCTCGTGCCCATGCAGAAGTAGATG GGCGCTCCACTGAGCCCCCAGCACCCAAGGCCCCGAGGCCAAAGTGGCCCAAGGTCAATGGCAAGAACTA TCCCAAGGCTTGGCAGGGGGCCAGCTCTGGGGAGGCTGCAGGCCCACCTGGCTGGCAAGGCTGCCCTGAT GAACCATGGCCATCTGCAACTCCTTGTGGGCCATCCGTCCAGCCATCTCATCAGCCCCTGAGCAAGGCTC TGGAGAGCCCTTTGGGGCTGCGCCCTCACCTGCCCCTGCTGATGGGTGGACAGGCGGCTCTGAAGCCGGA GCCTGGGCGCCCAGGCGAGGAGTCACCTGCCCCTAAGCAGGAACTGCATCAGCCCTCTTTCCCCACACCT CAGCTGTCGCCGCTGCCGATGCCTGGCAACCCCGCCGACTACAATGGCCTGTGTGTTGGGCCTGAGCTCA CTGCACTAGGCAGCTTCTACCTGTACTGTGGCCAAGAGGGGCTGCAGTGTGGGGGCTACTCGCCCTGCCC CATGCTTCCTGAGGGCAAGCTGTCCCCAGTGGCTGCACCTCACGAGGAGGGGCTCCTCTTAGCTCCGAGC TCAGTGCCCTCAGGCACCCCTTTCCAGCACCCTCCCTGGGGCTCCTCTCGCTACTGCTCTAGCGAGGACA CTGGAGTGAATGGCTACAGCATCTGCGGAGTGTTGCCCCTGTCTGTTACCCACGCTGGCACTACCTGTGG CGGCTGCCCATACAAAATGCCTTTTGCAGCAGGCTGCAGATCCCTGGGCCAGTTGGAATTTCCTCTCCCG GAAGCTGGCCACCCAGCCTCACCCGCCCACCCACTCCTGGGGTGCCCTGTACCCAGTGTGCCACCTGCAG CAGAGCCCGTCCCCCATCTTCAGACACCCACCTCGGAGCCCCAGACAGTAGCCCGTGCGTGCCCTCAGAG CGCCAAACCTCCCAGCGGTTCTAAGTCAGGTCTGCGCACAGGCTCCAGCTGCAGGCACACTGCAAGGAGC AAGGCTGCCCGCAGGCCTAGCCACCCCAAGCAGCCACGTGTCCAGCGCCCACGCCCTCGCCGCCGCCGTC GCCGCCGCACTAATGGCTGGGTACCTGTTGGGGCTGCGTGTGAGAAGGCTGTGTATGTCTTGGATGAGCC GGAGCCAGCCATCCGAAAGAGCTACCAGGCGGTAGAGCGGCATGGGGAGACAATCCGAGTCCGGGACACC GTCCTTCTCAAATCAGGCCCACGAAAGACCTCCACACCTTATGTGGCCAAGATCTCTGCCCTCTGGGAGA ACCCCGAGTCAGGAGAGCTGATGATGAGCCTCCTGTGGTATTACAGACCTGAGCACTTACAGGGAGGCCG CAGTCCCAGCATGCACGAGCCCTTGCAGAATGAAGTGTTTGCATCGCGACATCAGGACCAGAACAGTGTG GCCTGCATTGAGGAGAAGTGCTATGTGCTGACTTTTGCCGAGTACTGCAGGTTCTGTGCCATGGCCAAGC GCCGAGGTGAAGGCCTCCCCAGCCGAAAGACAGCACTGGTTCCCCCCTCTGCAGACTATTCCACCCCACC CCACCGCACAGTGCCAGAGGACACGGACCCTGAGCTGGTGTTCCTTTGCCGCCATGTCTATGACTTCCGC CACGGGCGCATCCTTAAGAACCCCCAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001301132
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301132.1, NP_001288061.1
RefSeq Size	4729 bp
RefSeq ORF	2340 bp
Locus ID	22893
UniProt ID	Q8TBE0
Gene Summary	Heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. May act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. Represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins. At specific stages of Listeria infection, in complex with TRIM28, corepresses interferon-stimulated genes, including IFNL1, IFNL2 and IFNL3.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (b) that is 1 aa shorter than isoform a.

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