STK25 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA	mBFP-Neo
Symbol	STK25
Locus ID	10494
Kit Components	KN203215G1, STK25 gRNA vector 1 in pCas-Guide CRISPR vector KN203215G2, STK25 gRNA vector 2 in pCas-Guide CRISPR vector KN203215BN-D, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette. Homologous arm and mBFP-Neo sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet GAGCCGTGCG CGGGCCGCGA CGCCGAGCAC CGCCCTCGCC GTCGCCTCCG GGCTTTCTCC GGTCGCTGCC GCCACCACCG TTGCTTCGCG GGCTGGGAGG CCCGGGGTCC CCGGGCGAAC AGAGGCTGCG GGTGGGAGCC TTCGCGGGCG CTGCAGAGCG GGCCGGGGGA GGCCGGGATG GAGCCCCGCG GAGGTGGGCG CGCTGCCGGG CGGGGCGCTT GCGGCGATTG GGGAGGGGGC AGAGGGAGGG GCCCGGGGCG GGGCTCGGTG GCGGGGACAC CGGGGCGGGG GCACCGGGAG GAAGCTGCCT TGGAAGAGGT GGGGGCGGCG ACGGGAGGGG CGGCGAGCCC CCGGGAGCCT GGGCGTTAGG CCCGGGCGTG GCGGGGCCCC GGCGGCCTGG GGGGTCTCCT GGGCCCCCCC CCACCCATGG AGCCCGCCGC CCCGGAGGTC GGTCTCAGAT GACTGAACTG GGCACCGAGC GCCCCTGGTG TCCCTCGCAG TGGACTGACG CCGCAGGGGC GAGCTAGCCG GCTCCGCGCC TCTCCGCGGG ATCCAGACGC CTCCTGGGGC TGCTGGCGGA GGGTCTGAGG CGGCGCGGCC GGCTCTGCCG TGTGCCTGTG GCGTTTTGCG TGGCAGAAGC TCGCAGGCCT CTAGAAACCG AGCCCCTGGT GGCTCCGCCT GTGCGAGGGC GGTGGAGGTG CGTGAAGAGA CACCGGCGCA GGGACGGGGC GCTGCCCCCA GCGCGGGGCG GGAACCTCAT GTCAGCATCC GAAGGCAGTG GAAGGTCGGG GGCCACCCTG CGGCCTCGGC CTGGGCCGCG GGTCATTCTG GCCGTGTTGG GCTGGGCAGG GGCTGTAGGG GAGACCCTGG CGGGCGCTGA GGGGCTGAGA TTGTGGAGAA GCAGATCTGA GTGGCCGGTG TGCAGGGAAA GCAGAGAACA CGTGTGTGCT TTGGCCCAGG GGAGGGTGAA AAGGCTGCTC TGTCAGGACC GTTTAACCCC TGCGACGGGC GGTTGCCCGA ACATGAGACT CATCTTTCTT CTTTAAAGAA GTCGTTTCCA CTATGGAGCC ACCTTTAAGC GTCTGGTGGG ACAGCCTCGG AGGGAGCCAC TACGGCGAGC CCTTAGCCTG GAATGAGGAC CAGCTGCTGG TGCCAAAGGG CTTGCCGGGG AAGGAGGGTG GGGTCAGCCA ACCTGGGGCC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001271977, NM_001271978, NM_001271979, NM_001271980, NM_001282305, NM_001282306, NM_001282307, NM_001282308, NM_006374, NR_073530, NR_073531, NR_073532, NR_073533
Synonyms	SOK1; YSK1
Summary	This gene encodes a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The encoded enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18. Multiple alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]

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