RPL8 (NM_033301) Human Mass Spec Standard

Specifications

Product Data
Description	RPL8 MS Standard C13 and N15-labeled recombinant protein (NP_150644)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC201368
Predicted MW	28 kDa
Protein Sequence	Sequence Link (showhide) >RC201368 protein sequence Red=Cloning site Green=Tags(s) MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFK KRTELFIAAEGIHTGQFVYCGKKAQLNVGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHN PETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHP FGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_150644
RefSeq Size	967
RefSeq ORF	771
Synonyms	L8
Locus ID	6132
Cytogenetics	8q24.3
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Protein Pathways	Ribosome

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