B3GALT5 (NM_033173) Human Over-expression Lysate

Name: B3GALT5 (NM_033173) Human Over-expression Lysate
SKU: LY409693
Price: 3080.00

CAT＃: LY409693

Transient overexpression lysate of UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 5

Size: 20 ug 100 ug

Datasheet

SDS

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LY409693 is the same product as LY429860.

CNY 3,080.00

货期*

3周

Size

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Specifications

Product Data
Description	Transient overexpression lysate of UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 5
Species	Human
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	TrueORF Clone RC211183
Tag	C-Myc/DDK
Detection Antibodies	Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
Predicted MW	36.2 kDa
Components	1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Storage	The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Preparation	HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq	NP_149363
Locus ID	10317
Cytogenetics	21q22.2
Protein Families	WB
Protein Pathways	Glycosphingolipid biosynthesis - globo series, Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
Western Blot Protocols
FAQs
Over-expression Cell Lysate
SDS
Verify Lysate Provided SDS Buffer Protein Lysate

Resources

蛋白相关资源
视频教程：蛋白表达宿主为何重要网络讲座视频：过表达裂解液作为WB的阳性对照产品概述视频：过表达裂解液可作为WB的阳性对照视频教程：敲除（KO）裂解物和抗体特异性产品手册：蛋白

Other Versions

SKU	Description	Size	Price
LC409690	B3GALT5 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug 产品选项验证失败所有选项都是必需的	CNY 900.00
LC409691	B3GALT5 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug 产品选项验证失败所有选项都是必需的	CNY 900.00
LC409692	B3GALT5 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug 产品选项验证失败所有选项都是必需的	CNY 900.00
LC409693	B3GALT5 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug 产品选项验证失败所有选项都是必需的	CNY 900.00
LC416890	B3GALT5 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug 产品选项验证失败所有选项都是必需的	CNY 900.00
LY409690	Transient overexpression lysate of UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 2	100 ug 产品选项验证失败所有选项都是必需的	CNY 3,080.00
LY409691	Transient overexpression lysate of UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 3	100 ug 产品选项验证失败所有选项都是必需的	CNY 3,080.00
LY409692	Transient overexpression lysate of UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 4	100 ug 产品选项验证失败所有选项都是必需的	CNY 3,080.00
LY416890	Transient overexpression lysate of UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 1	100 ug 产品选项验证失败所有选项都是必需的	CNY 3,080.00
PH311183	B3GALT5 MS Standard C13 and N15-labeled recombinant protein (NP_149363)	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,250.00
PH313317	B3GALT5 MS Standard C13 and N15-labeled recombinant protein (NP_149362)	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,250.00
PH314669	B3GALT5 MS Standard C13 and N15-labeled recombinant protein (NP_149360)	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,250.00
PH314721	B3GALT5 MS Standard C13 and N15-labeled recombinant protein (NP_149361)	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,250.00
PH315430	B3GALT5 MS Standard C13 and N15-labeled recombinant protein (NP_006048)	10 ug 产品选项验证失败所有选项都是必需的	CNY 19,520.00
TP311183	Purified recombinant protein of Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 5, 20 µg	20 ug 产品选项验证失败所有选项都是必需的	CNY 2,900.00 CNY 6,650.00
TP313317	Purified recombinant protein of Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 4, 20 µg	20 ug 产品选项验证失败所有选项都是必需的	CNY 2,900.00 CNY 6,650.00
TP314669	Purified recombinant protein of Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 2, 20 µg	20 ug 产品选项验证失败所有选项都是必需的	CNY 2,900.00 CNY 6,650.00
TP314721	Purified recombinant protein of Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 3, 20 µg	20 ug 产品选项验证失败所有选项都是必需的	CNY 2,900.00 CNY 6,650.00
TP315430	Recombinant protein of human UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 (B3GALT5), transcript variant 1, 20 µg	20 ug 产品选项验证失败所有选项都是必需的	CNY 2,900.00 CNY 6,650.00