Glyoxalase I / GLO1 (1-184) Human Protein
CAT#: AR09139PU-N
Glyoxalase I / GLO1 (1-184) human recombinant protein, 0.1 mg
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CNY 8,000.00
Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM
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Predicted MW | 20.7 kDa |
Concentration | lot specific |
Purity | >90% by SDS - PAGE |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol |
Preparation | Liquid purified protein |
Protein Description | Recombinant human GLO1 protein was expressed in E.coli and purified by using conventional chromatography techniques. |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Bioactivity | Biological: >1.7 units/ml (please enquire for specific batch value). One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25°C. Specific activity was expressed in units/mg of protein. Activity Assay Final assay concentrations: In 1.5ml reaction mix, the final concentrations are 79 mM potassium phosphate, 0.033%(w/v) reduced glutathine, 0.003% (w/v) bovine serum albumin, 0.033% methylglyoxal (w/v) and recombinant glyoxalase I (0.5ug, 1ug, 2ug). 1. Prepare 1.45ml reaction buffer without recombinant protein into suitable container (for each protein concentration). |
Reference Data | |
RefSeq | NP_006699 |
Locus ID | 2739 |
UniProt ID | Q04760, X5DNM4 |
Cytogenetics | 6p21.2 |
Synonyms | GLOD1; GLYI; HEL-S-74 |
Summary | The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008] |
Protein Pathways | Pyruvate metabolism |
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