Nelfcd (NM_020580) Mouse Recombinant Protein
CAT#: TP509209
Purified recombinant protein of Mouse negative elongation factor complex member C/D, Th1l (Nelfcd), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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Specifications
Product Data | |
Species | Mouse |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>MR209209 representing NM_020580
Red=Cloning site Green=Tags(s) MAGPAPGTIMGEDYFGNASEWGEEADGGQHQEDDSGEGEDDAEVQQECLHKFSTRDYIMEPSIFNTLKRY FQAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLKSLLIKHFDPRKADSIFTEE GETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLISDAGYQGEITSVSTACQQLEVFSRVLRTSL ATILDGGEENLEKNLPEFAKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRVAQEVQRFAQEKGHDASQI TLALGTAASYPRACQALGAMLSRGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGAKIN QDHKHKYIHILAYAASVVETWKKNKRVSIGKDELKSTSKAIETVHNLCCNENKGASELVAELSTLYQCIR FPVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCHQLLHPQVLQLLVKLFETEHSQLDVMEQ LELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKLDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILEN DSIAGTIKAEGEHDPVTEFIAHCKSNFIVVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-MYC/DDK |
Predicted MW | 66.3 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C after receiving vials. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_065605 |
Locus ID | 57314 |
UniProt ID | Q922L6 |
Refseq Size | 2279 |
Cytogenetics | 2 H4 |
Refseq ORF | 1773 |
Synonyms | 2410003I03Rik; C77797; Th1; Th1l |
Summary | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (By similarity). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity).[UniProtKB/Swiss-Prot Function] |
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